Expression of native GABA(A) receptors in Xenopus oocytes injected with rat brain synaptosomes

Journal of Neurochemistry

Volumn 67, Issue 5, 1996, Pages 2212-2214

  Sanna, Enrico ^a   Motzo, Costantino ^b   Murgia, Antonella ^b   Amato, Francesco ^b   Deserra, Tiziana ^b   Biggio, Giovanni ^b  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b NONE

Author keywords

Chloride currents; GABA(A) receptors; Heterologous expression; Rat brain synaptosomes; Voltage clamp; Xenopus oocytes

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; BENZODIAZEPINE; NICOTINIC RECEPTOR; 3 AMINO 2 (3 CARBOXYPROPYL) 6 (4 METHOXYPHENYL)PYRIDAZINIUM BROMIDE; 4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID RECEPTOR BLOCKING AGENT; ALFAXALONE; ANESTHETIC AGENT; CHLORIDE; CHLORIDE CHANNEL; DIAZEPAM; PICROTOXIN; PREGNANEDIONE; PYRIDAZINE DERIVATIVE; ZINC CHLORIDE; ZINC DERIVATIVE;

ANIMAL TISSUE; ARTICLE; BRAIN SYNAPTOSOME; CHLORIDE CURRENT; ELECTROPHYSIOLOGY; MALE; MEMBRANE VESICLE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; VOLTAGE CLAMP; XENOPUS LAEVIS; ANIMAL; BIOSYNTHESIS; BRAIN; CELL MEMBRANE; CELL MEMBRANE POTENTIAL; DRUG EFFECT; FEMALE; PHYSIOLOGY; SYNAPTOSOME;

ANIMALIA; ANURA; XENOPUS LAEVIS;

ANESTHETICS; ANIMALS; BRAIN; CELL MEMBRANE; CHLORIDE CHANNELS; CHLORIDES; DIAZEPAM; FEMALE; GABA ANTAGONISTS; GAMMA-AMINOBUTYRIC ACID; MEMBRANE POTENTIALS; OOCYTES; PICROTOXIN; PREGNANEDIONES; PYRIDAZINES; RATS; RECEPTORS, GABA-A; SYNAPTOSOMES; XENOPUS LAEVIS; ZINC COMPOUNDS;

EID: 0029661955     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.67052212.x     Document Type: Article

References (15)

1. Amin J., Dickerson I. M., and Weiss D. S. (1994) The agonist binding site of the γ-aminobutyric acid type A channel is not formed by the extracellular cysteine loop. Mol. Pharmacol. 45, 317-323.
2. Blair L. A. C., Levitan E. S., Marshall J., Dionne V. E., and Barnard E. A. (1988) Single subunits of the GABAA receptor form ion channels with properties of the native receptor. Science 242, 577-579.
3. Dildy-Mayfield J. E. and Harris R. A. (1992) Acute and chronic ethanol exposure alters the function of hippocampal kainate receptors expressed in Xenopus oocytes. J. Neurochem. 58, 1569-1572.
4. Gray E. G. and Whittaker V. P. (1972) The isolation of nerve endings from brain: an electron-microscopic study of cell fragments derived by homogenization and centrifugation. J. Anat. 96, 79-88.
5. Julius D., MacDermott A. B., Axel R., and Jessell T. M. (1988) Molecular characterization of a functional cDNA encoding the serotonin 1c receptor. Science 241, 558-564.
6. Lester H. A. (1988) Heterologous expression of excitability proteins: route to more specific drugs? Science 241, 1057-1063.
7. Lowry O. H., Rosebrough N. J., Farr A. L., and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
8. - channels in Xenopus oocytes injected with Torpedo electroplaque membranes. Proc. Natl. Acad. Sci. USA 92, 5224-5228.
9. Masu Y., Nakayama K., Tamaki H., Harada Y., Kuno M., and Nakanishi S. (1987) cDNA cloning of bovine substance-K receptor through oocyte expression system. Nature 329, 836-839.
10. Morales A., Aleu J., Ivorra I., Ferragut J. A., Gonzalez-Ros J. M., and Miledi R. (1995) Incorporation of reconstituted acetylcholine receptors from Torpedo into oocyte membrane. Proc. Natl. Acad. Sci. USA 92, 8468-8472.
11. Sanna E., Dildy-Mayfield J. E., and Harris R. A. (1994) Ethanol inhibits the function of 5-hydroxytryptamine type 1c and muscarinic M1 G protein-linked receptors in Xenopus oocytes expressing brain mRNA: role of protein kinase C. Mol. Pharmacol. 45, 1004-1012.
12. A receptors: influence of receptor subunits. J. Pharmacol. Exp. Ther. 274, 353-360.
13. A receptor shows a ligand-gated receptor super-family. Nature 328, 221-227.
14. A receptor subtypes. Pharmacol. Rev. 47, 181-234.
15. Snutch T. P. (1988) The use of Xenopus oocytes to probe synaptic communication. Trends Neurosci. 11, 250-256.