Cloning of VEGF 165 into expression vector for protein production

Australian and New Zealand Journal of Ophthalmology

Volumn 24, Issue 2 SUPPL., 1996, Pages 81-84

  Tuffin, Meagan J ^a   Rakoczy, Piroska E ^a   Lai, May C ^a   Papadimitriou, John M ^a,b   Constable, Ian J ^a  

^a PATHWEST LABORATORY MEDICINE   (Australia)

^b UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

VASCULOTROPIN; ENDOTHELIAL CELL GROWTH FACTOR; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; LYMPHOKINE; VASCULAR ENDOTHELIAL GROWTH FACTOR A, HUMAN; VASCULOTROPIN A;

ANGIOGENESIS; CONFERENCE PAPER; ESCHERICHIA COLI; PROTEIN SYNTHESIS; AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; POLYACRYLAMIDE GEL ELECTROPHORESIS; RAT;

ANIMALS; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTHELIAL GROWTH FACTORS; ESCHERICHIA COLI; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; LYMPHOKINES; RATS; RECOMBINANT FUSION PROTEINS; VASCULAR ENDOTHELIAL GROWTH FACTOR A; VASCULAR ENDOTHELIAL GROWTH FACTORS;

EID: 0029661925     PISSN: 08149763     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (9)

1. Neufeld G, Tessler S, Gitay-Goren H, Cohen T, Levi BZ. Vascular endothelial growth factor and its receptors. Prog Growth Factor Res 1994;5:89-92.
2. Michaelson IC. The mode of development of the vascular system of the retina, with some observations of its significance for certain retinal diseases. Trans Ophthalmol Soc UK 1948;68:137-80.
3. Pierce EA, Avery RL, Foley ED, Aiello LP, Smith LE. Vascular endothelial growth factor/vascular permeability factor expression in a mouse model of retinal neovascularisation. Proc Nad Acad Sci USA 1995;92:905-9.
4. Smith LEH, Pierce EA, Aiello LP, Foley ED, Sullivan R, Rook SI, et al. Inhibition of proliferative retinopathy using antisense phosphorothioate oligonucleotides against vascular endothelial growth factor (VEGF/VPF). Invest Ophthalmol Vis Sci 1995;36:S871.
5. Malecaze F, Clamens S, Simorre-Pinatel V, Mathis A, Chollet P, Favard C, et al. Detection of vascular endothelial growth factor messenger RNA and vascular endothelial growth factor-like activity in proliferative diabetic retinopathy. Arch Ophthalmol 1994;112:1476-82.
6. Dastgheib K, Li Q, Chan C-C, Roberge FG, Csaky K, Green WR. Vascular endothelial growth factor (VEGF) in neovascular age-related macular degeneration. Invest Ophthalmol Vis Sci 1995;36:S102.
7. Aiello LP, Avery RL, Arrigg PG, Keyt BA, Jampel HD, Shah ST, et al. Vascular endothelial growth factor in ocular fluid of patients with diabetic retinopathy and other retinal disorders. N Engl J Med 1994;331:1480-7.
8. Breier G, Albrecht U, Sterrer Ss, Risau W. Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development 1992;114:521-32.
9. Smith DB, Johnson KS. Single step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 1988;67:31-40.