Paramacium has two regulatory subunits of cyclic AMP-dependent protein kinase, one unique to cilia

Journal of Eukaryotic Microbiology

Volumn 43, Issue 4, 1996, Pages 356-362

  Hochstrasser, Martin ^a   Carlson, Gail L ^a,b   Walczak, Claire E ^a,c   Nelson, David L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b ST OLAF COLLEGE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Ciliate; motility; phosphorylation

Indexed keywords

CILIOPHORA; EUKARYOTA; PARAMECIUM;

CYCLIC AMP DEPENDENT PROTEIN KINASE;

ANIMAL; ARTICLE; ENZYMOLOGY; EUKARYOTIC FLAGELLUM; GEL CHROMATOGRAPHY; IMMUNOBLOTTING; ISOLATION AND PURIFICATION; METABOLISM; PARAMECIUM TETRAURELIA; PHOSPHORYLATION; REGULATORY SEQUENCE;

ANIMALS; CHROMATOGRAPHY, AGAROSE; CHROMATOGRAPHY, GEL; CILIA; CYCLIC AMP-DEPENDENT PROTEIN KINASES; IMMUNOBLOTTING; PARAMECIUM TETRAURELIA; PHOSPHORYLATION; REGULATORY SEQUENCES, NUCLEIC ACID;

EID: 0029658942     PISSN: 10665234     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1550-7408.1996.tb04000.x     Document Type: Article

References (32)

1. Adoutte, A., Ramanathan, R., Lewis, R. M., Dute, R. R., Lin, K.-Y., Kung, C. & Nelson, D.L. 1980. Biochemical studies of the excitable membrane of Paramecium tetraurelia. III. Proteins of cilia and ciliary membranes. J. Cell Biol., 84:717-738.
2. Bonini, N. M. & Nelson, D. L. 1988. Differential regulation of Paramecium ciliary motility by cAMP and cGMP. J. Cell Biol., 106: 1615-1623.
3. Bonini, N. M. & Nelson, D. L. 1990. Phosphoproteins associated with cyclic nucleotide stimulation of ciliary motility in Paramecium. J. Cell Sci., 95:219-230.
4. Bonini, N. M., Gustin, M. C. & Nelson, D. L. 1986. Regulation of ciliary motility by membrane potential in Paramecium: a role for cyclic AMP. Cell Motil. Cytoskel., 6:256-272.
5. Cadd, G. G., Uhler, M. D. & McKnight, G. S. 1990. Holoenzymes of cAMP-dependent protein kinase containing the neural form of type I regulatory subunit have an increased sensitivity to cyclic nucleotides. J. Biol. Chem., 265:19502-19506.
6. Carlson, G. L. & Nelson, D. L. 1995. Isolation and characterization of protein kinases from Paramecium cilia. Meth. Cell Biol., 47: 473-480.
7. de Gunzburg, J., Part, D., Guiso, N. & Véron, M. 1984. An unusual adenosine 3′,5′-phosphate-dependent protein kinase from Dictyostelium discoideum. Biochemistry 23:3805-3812.
8. Hamasaki, T., Barkalow, K., Richmond, J. & Satir, P. 1991. Cyclic AMP-stimulated phosphorylation of an axonemal polypeptide that copurifies with the 22S dynein arm regulates microtubule translocation velocity and swiming speed in Paramecium. Proc. Natl. Acad. Sci. USA, 88:7918-7922.
9. Hamasaki, T., Murtaugh, T. J., Satir, B. H. & Satir, P. 1989. In vitro phosphorylation of Paramecium axonemes and permeabilized cells. Cell Motil. Cytoskel., 12:1-11.
10. Hennessey, T., Machemer, H. & Nelson, D. L. 1985. Injected cyclic AMP increases ciliary beat frequency in conjunction with membrane hyperpolarization. Eur. J. Cell. Biol., 36:153-156.
11. Hochstrasser, M. & Nelson, D. L. 1989. Cyclic AMP-dependent protein kinase in Paramecium tetraurelia. J. Biol. Chem., 264:14510-14518.
12. Kapoor, C. L., Beavo, J. A. & Steiner, A. L. 1979. Radioimmunoassay of the regulatory subunit of type I cAMP-dependent protein kinase. J. Biol. Chem., 254:12427-12432.
13. Klumpp, S. & Schultz, J. E. 1991. Identification of a 42 kDa protein as a substrate of protein phosphatase 1 in cilia from Paramecium. FEBS Lett., 288:60-64.
14. Klumpp, S., Cohen, P. & Schultz, J. E. 1990. Chromatographic separation of four Ser/Thr-protein phosphatases from solubilized ciliary membranes of Paramecium tetraurelia by heparin-Sepharose. J. Chromatogr., 521:179-186.
15. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227:680-685.
16. Lewis, R. M. & Nelson, D. L. 1980. Biochemical studies of the excitable membrane of Paramecium tetraureha. VI. Endogenous protein substrates for in vitro and in vivo phosphorylation in cilia and ciliary membranes. Biochim. Biophys. Acta, 615:341-353.
17. Mason, P. A. & Nelson, D. L. 1989. Cyclic AMP-dependent protein kinases of Paramecium. I. Chromatographic and physical properties of the enzymes from cilia. Biochim. Biophys. Acta, 1010:108-115.
18. Miglietta, L. A. P. & Nelson, D. L. 1988. A novel cGMP-dependent protein kinase from Paramecium. J. Biol. Chem. 263:16096-16105.
19. Morrissey, J. H. 1981. Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced sensitivity. Anal. Biochem., 117: 307-310.
20. Murofushi, H. 1974. Protein kinases in Tetrahymena cilia II. Partial purification and characterization of adenosine 3′,5′-monophosphate-dependent and guanosine 3′,5′-dependent protein kinases. Biochim. Biophys. Acta, 370:130-139.
21. Mutzel, R., Lacombe, M.-L., Simon, M.-N., de Gunzburg, J. & Véron, M. 1987. Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum. Proc. Natl. Acad. Sci. USA, 84:6-10.
22. Nakaoka, Y. & Ooi, H. 1985. Regulation of ciliary reversal in Triton-extracted Paramecium by calcium and cyclic adenosine monophosphate. J. Cell Sci, 77:185-195.
23. Noguchi, M., Nakamura, Y. & Okamoto, K. 1991. Control of ciliary orientation in ciliated sheets from Paramecium: differential distribution of sensitivity to cyclic nucleotides. Cell Motil. Cytoskel., 20: 38-46.
24. Schultz, U. E. & Jantzen, H. M. 1980. Cyclic nucleotide-dependent protein kinases from cilia of Paramecium tetraurelia. FEBS Lett., 116:75-78.
25. Taylor, S. S., Buechler, J. A. & Yonemoto, W. 1990. cAMP-dependent protein kinase: framework for a diverse family of regulatory enzymes. Ann. Rev. Biochem., 59:971-1005.
26. Towbin, H., Staehelin, T. & Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: prodecure and some applications. Proc. Natl. Acad. Sci. USA, 76:4350-4354.
27. 2+ and cyclic nucleotides. Biochim. Biophys. Acta, 966:84-93.
28. Van Wagtendonk, W. J. 1974. Paramecium: a current survey. In: Van Wagtendonk, W. J. (ed.), Paramecium: A Current Survey. Elsevier Scientific, New York. Pp. 339-376.
29. Van Houten, J. L., Cote, B. L., Zhang, J., Baez, J. & Gagnon, M. L. 1991. Studies of the cyclic adenosine monophosphate chemoreceptor of Paramecium. J. Membr. Biol. 119:15-24.
30. Walczak, C. E. & Nelson, D. L. 1993. In vitro phosphorylation of ciliary dyneins by protein kinases from Paramecium. J. Cell Sci., 106:1369-1376.
31. Walter, U., Uno, I., Liu, A. Y.-C. & Greengard, P. 1977. Study of autophosphorylation of isoenzymes of cyclic AMP-dependent protein kinases. J. Biol. Chem., 252:6588-6590.
32. Wang, Y., Scott, J. D., McKnight, G. S. & Krebs, E. G. 1991. A constitutively active holoenzyme form of the cAMP-dependent protein kinase. Proc. Natl. Acad. Sci. USA, 88:2446-2450.