[1-13-C]glucose metabolism in brain cells: Isotopomer analysis of glutamine from cerebellar astrocytes and glutamate from granule cells

Developmental Neuroscience

Volumn 18, Issue 5-6, 1996, Pages 460-468

  Merle, Michel ^a   Martin, Magali ^a   Villégier, Annick ^a   Canioni, Pani ^a  

^a CNRS University Bordeaux Segalen   (France)

Author keywords

Cerebellar astrocytes; Glutamine; Granule cells; Isotopomers; Mathematical modeling; Nuclear magnetic resonance; Tricarboxylic acid cycle; 1 13 C glucose

Indexed keywords

CELL EXTRACT; CITRIC ACID; FUMARIC ACID; GLUTAMIC ACID; GLUTAMINE; OXALOACETIC ACID; PERCHLORIC ACID; PYRUVATE CARBOXYLASE; PYRUVATE DEHYDROGENASE; PYRUVIC ACID; TRICARBOXYLIC ACID; CARBON; GLUCOSE;

ANIMAL CELL; ASTROCYTE; BRAIN CELL; BRAIN NERVE CELL; CARBON NUCLEAR MAGNETIC RESONANCE; CITRIC ACID CYCLE; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; EXTRACELLULAR FLUID; GLUCOSE METABOLISM; GRANULE CELL; ISOTOPE LABELING; MATHEMATICAL MODEL; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; ARTICLE; BIOLOGICAL MODEL; CELL CULTURE; CEREBELLUM; CYTOLOGY; METABOLISM; NERVE CELL;

ANIMALS; ASTROCYTES; CARBON ISOTOPES; CELLS, CULTURED; CEREBELLUM; CITRIC ACID CYCLE; GLUCOSE; GLUTAMIC ACID; GLUTAMINE; MODELS, BIOLOGICAL; NEURONS; RATS;

EID: 0029658506     PISSN: 03785866     EISSN: 14219859     Source Type: Journal    
DOI: 10.1159/000111441     Document Type: Article

References (28)

1. Nürnberger A: Amino acid uptake in neuronal and glial cell fractions from rabbit cerebral cortex. Brain Res 1971;31:169-178.
2. Shank RP. Bennett GS. Freytag SO. Campbell GL: Pyruvate carboxylase: An astrocyte-specific enzyme implicated in the replenishment of amino acid neurotransmitler pools. Brain Res 1985;329:364-367.
3. Norenberg MD. Martincz-Hemandez A: Fine structural location of glutamine synthetase in astrocytes of rat brain. Brain Res 1979; 161: 303-310.
4. Peng L, Hertz L. Huang R. Sonncvvald U. Petersen SB, Westergaard N, Larsson O, Schousboc A: Utilization of glutamine and of TCA cycle constituents as precursors for transmitter glutamate and GABA. Dev Neurosci 1993;15:367-377.
5. Brainard JB. Kyner E, Rosenberg GA: 13C nuclear magnetic resonance evidence for g-antinobutyric acid formation via pyruvate carboxylase in rat brain: A metabolic basis for compartmentalion. J Neuro Client 1989:53: 1285-1292.
6. Mason GF. Rothman DL. Bchar KL, Shulmun RG: NMR determination of the TCA cycle rate and oc-ketoglutarate/glutamate exchange rale in rat brain. J Cereb Blood Flow Metab 1992; 12:434-447.
7. Shank R. Leo GC, Zielkc HR: Cerebral metabolic comparttnentation as revealed by nuclear magnetic resonance analysis of D[1-13C]glu-cosc metabolism. J Neurochem 1993:61:315- 323.
8. Kanamatsu T. Tsukada Y: Measurement of amino acid metabolism derived from [1-3CJ-glucosc in the rat brain using’13C magnetic resonance spectroscopy. Neurochem Res 1994; 19:603-612.
9. Cerdan S. KUnnccke B. Scelig J: Cerebral metabolism of [I. 2-13C,]acetate as detected by in vivo and in vitro 13C NMR. J Biol Chem 1990;265:12916-12926.
10. Lapidot A. Gopher A: Cerebral metabolic compartmentalion: Estimation of glucose flux via pyruvate carboxylase/pyruvate dehydrogenase by 13CNMR isotopomer analysis of f7-[U-13CJglucose metabolites. J Biol Chem 1994; 269:27198-27208.
11. Shank RP, Nicklas WJ: y-aminobutyric acid formation from glucose: metabolic pathway. J Neurochem 1990;55:352-353.
12. ?CJO-glucose metabolism in cultured astrocytes and neurons using nuclear magnetic resonance spectroscopy. Dev Neurosci 1993;15:282-288.
13. Martin M. Portais JC, Labouosse J. Canioni P. Merle M: [l-13C]glucosc metabolism in rat cerebellar granule cells and astrocytes in primary culture: Evaluation of flux parameters by '13C-and 'H-N MR spectroscopy. Eur J Biochem 1993:217:617 -625.
14. Goridis C. Joher MA. Hirsh M. Schachner M: Cell surface proteins of cultured brain cells and their recognition by anti-cerebellum (anti N-S4) anti-serum. J Neurochem 1978:31:531-539.
15. Patel AJ. Hunt A. Tahourdin SM: Regulation of in vivo glutamine synthetase activity by glucocorticoids in the developing brain. Dev Brain Res 1983:10: 83-91.
16. Voisin PJ. Girault JM, Labouessc J. Viraiellc OM: β-Adrencrgic receptors of cerebellar
17. astrocytes in culture: intact cells versus membrane preparation. Brain Res 1987:404:65-79.
18. Malloy CR. Sherry AD. Jeffrey FMH: Analysis of tricarboxylic acid cycle of the heart using 13C isotopeisomers. Am J Physiol 1990:259: H987-H995.
19. IS Sherry AD. Sumegi B. Miller B. Cotlam GL. Gavva S. Jones GJ. Malloy CR: Orientation-conserved transfer of symmetric Krebs cycle intermediates in mammalian tissue. Biochemistry 1994:33:6268-6275.
20. Merle M. Pérou M. Valeins H. Canioni P: Asymmetry conservation during conversion of succinyl-CoA into malate in the TCA cycle of C6 glioma cells. Proe 2nd Meet Soc Magnetic Resonance. San Francisco. 1994. p 1318.
21. Duggleby G: Estimation of the reliability of parameters obtained by non-linear regression. EurJ Biochcm 1980;109:93-96.
22. Martin M. Portais JC, Voisin P, Rousse N. Canioni P. Merle M: Comparative analysis of 13C-enrichcd metabolites released in the medium of cerebellar and cortical astrocytes incubated with [l-13Clglucosc. Eur J Biochcm 1995:23: 697-703.
23. Martin-Biran M: Etude par spectroscopic de RMN du métabolisme des neurones et des astrocytes en culture primaire: Thèse. Université de Bordeaux II. 1994.
24. Brookes N: Interaction between the glutamine cycle and the uptake of large neutral amino acids in astrocytes. J Ncurochcm 1993:60: 1923- 1928.
25. Merle M. Pianet I. Canioni P. Labouessc J: Comparative 31P and 'H NMR studies on rat astrocytes and C6 glioma cells in culture. Biochimie 1992:74:919-930.
26. Urenjak J, Williams SR. Gadian DG. Noble M: Proton nuclear magnetic resonance spectroscopy unambiguously identifies different neural cell types. J Ncurosci 1993; 13:981 - 989.
27. Brand A. Engclmann J. Lcibfritz D: A 13C NMR study on fluxes into the TCA cycle of neuronal and glial tumor cell lines and primary cells. Biochimie 1992:74:941 -948.
28. Yu ACH, Drejer J, Hertz L. Schousboe A: Pyruvate carboxylase activity in primary cultures of astrocytes and neurons. J Ncurochem 1983;41:1484-1487.