Detection of cytomegalovirus in blood donors by PCR using the digene SHARP signal system assay: Effects of sample preparation and detection methodology

Journal of Clinical Microbiology

Volumn 34, Issue 1, 1996, Pages 29-33

  Krajden, Mel ^a,b,d   Shankaran, Prema ^a   Bourke, Caroline ^a   Lau, Wendy ^c  

^a TORONTO GENERAL HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c Canadian Red Cross Society   (Canada)

^d Toronto General Hosptial   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS;

ARTICLE; BLOOD DONOR; BLOOD TRANSFUSION; CYTOMEGALOVIRUS; DNA DETERMINATION; GENE AMPLIFICATION; HUMAN; MORBIDITY; MORTALITY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; VIRUS DETECTION; VIRUS TRANSMISSION;

BLOOD DONORS; BLOOD TRANSFUSION; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA PRIMERS; DNA, VIRAL; EVALUATION STUDIES; HUMAN; POLYMERASE CHAIN REACTION; SUPPORT, NON-U.S. GOV'T; VIROLOGY;

EID: 0029655415     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.1.29-33.1996     Document Type: Article

References (25)

1. Bevan, I. S., R. A. Daw, P. J. R. Day, F. A. Ala, and M. R. Walker. 1991. Polymerase chain reaction for detection of human cytomegalovirus infection in a blood donor population Br. J Haematol. 78:94-99.
2. Bitsch, A., R. Kirchner, R. Dupke, and G. Bein. 1992. Failure to detect human cytomegalovirus DNA in peripheral blood leukocytes of healthy blood donors by the polymerase chain reaction. Transfusion (Bethesda) 32:612-617.
3. Boeckh, M., P. M. Woogerd, T. Stevens -Ayers, C. G. Ray, and R. A. Bowden. 1994. Factors influencing detection of quantitative cytomegalovirus antigenemia J. Clin. Microbiol. 32:832-834
4. Bowden, R. A. 1995. Transfusion-transmitted cytomegalovirus infection. Hematol. Oncol. Clin N. Am. 9:155-165.
5. Chan, A., J. Zhao, and M. Krajden. 1994. Polymerase chain reaction kinetics when using a positive internal control target to quantitatively detect cytomegalovirus target sequences. J. Virol. Methods 48:223-236.
6. Chou, S. 1992. Effect of interstrain variation on diagnostic DNA amplification of the cytomegalovirus major immediate-early gene region. J. Clin. Microbiol. 30:2307-2310.
7. Cone, R. W., A. C. Hobson, and M.-L. W. Huang. 1992. Coamplified positive control detects inhibition of polymerase chain reactions. J. Clin. Microbiol. 30:3185-3189.
8. Coupland, R. W. 1994 Quality control, analytic sensitivity, and the polymerase chain reaction. J Clin. Immunoassay 17:237-242.
9. Demmler, G. J., G. J. Buffone, C. M. Schimbor, and R. A. May. 1988. Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification. J. Infect. Dis. 158:1177-1184.
10. Espy, M. J., R. Patel, C. V. Paya, and T. F. Smith. 1995. Comparison of three methods for extraction of viral nucleic acids from blood cultures J. Clin. Microbiol 33:41-44
11. Hillyer, C. D., R. K. Emmens, M. Zago-Novaretti, and E. M. Berkman. 1994. Methods for the reduction of transfusion-transmitted cytomegalovirus infection: filtration versus the use of seronegative donor units. Transfusion (Bethesda) 34:929-934.
12. Hillyer, C. D., D. R. Snydman, and E. M. Berkman. 1990. The risk of cytomegalovirus infection in solid organ and bone marrow transplant recipients, transfusion of blood products. Transfusion (Bethesda) 30:659-666.
13. Jiwa, N. M., G. W. Van Gemert, A. K. Raap, F. M. Van De Rijke, A. Mulder, P. F. Lens, M. M. M. Salimans, F. E. Zwaan, W. Van Dorp, and M. Van Der Ploeg. 1989. Rapid detection of human cytomegalovirus DNA in peripheral blood leukocytes of viremic transplant recipients by the polymerase chain reaction. Transplantation 48:72-76
14. Krajden, M., S. Walmsley, P. Shankaran, R. Chua, and T. Mazzulli. 1995. Detection of cytomegalovirus (CMV) viremia in HIV infected patients by pp65 antigenemia and semi-quantitative PCR using the Digene Sharp Signal assay (DSSA), abstr C-219. p. 38. In Abstracts of The 95th General Meeting of American Society for Microbiology 1995. American Society for Microbiology, Washington. D.C
15. Noordhoek, G. T., A. H. J. Kolk, G. Bjune, D. Catty, J. W. Dale, P. E. M. Fine, P. Godfrey-Faussett, S.-N. Cho, T. Shinnick, S. B. Svenson, S. Wilson, and J. D. A. van Embden. 1994 Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories J. Clin. Microbiol. 32:277-284.
16. Preiksaitis, J. A., L. Brown, and M. McKenzie. 1988. The risk of cytomegalovirus infection in seronegative transfusion recipients not receiving exogenous immunosuppression J. Infect. Dis. 157:523-529.
17. Smith, K. L., J. K. Kulski, T. Cobain, and R. A. Dunstan. 1993. Detection of cytomegalovirus in blood donors by the polymerase chain reaction. Transfusion (Bethesda) 33:497-503.
18. Storch, G. A., R. S. Buller, T. C. Bailey, N. A. Ettinger, T. Langlois, M. Gaudreault-Keener, and P. L. Welby. 1994. Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients J Clin Microbiol 32:997-1003
19. The, T. H., M. Van Der Ploeg, A. P. Van Den Berg, A. M. Vlieger, M. Van Der Giessen, and W. J. Van Son. 1992. Direct detection of cytomegalovirus in peripheral blood leukocytes - a review of the antigenemia assay and polymerase chain reaction. Transplantation 54:193-198.
20. Weiss, J. B. 1995 DNA probes and PCR for diagnosis of parasitic infections. Clin. Microbiol. Rev. 8:113-130.
21. Wilhelm, J. A., L. Matter, and K. Schopfer. 1986. The risk of transmitting cytomegalovirus to patients receiving blood transfusions. J Infect Dis. 154:169-171
22. Zaaijer, H. L., H. T. M. Cuypers, H. W. Reesink, I. N. Winkel, G. Gerken, and P. N. Lelie. 1993, Reliability of polymerase chain reaction for detection of hepatitis C virus Lancet 341:722-724.
23. Zaaijer, H. L., F. ter Borg, H. T. M. Cuypers, M. C. A. H. Hermus, and P. N. Lelie. 1994. Comparison of methods for detection of hepatitis B virus DNA. J. Clin. Microbiol. 32:2088-2091.
24. Zhang, L. J., P. Hanff, C. Rutherford, W. H. Churchill, and C. S. Crumpacker. 1995. Detection of human cytomegalovirus DNA, RNA, and antibody in normal donor blood. J. Infect. Dis. 171:1002-1006.
25. Zipeto, D., S. Morris, C. Hong, A. Dowling, R. Wolitz, T. C. Merigan, and L. Rasmussen. 1995. Human cytomegalovirus (CMV) DNA in plasma reflects quantity of CMV DNA present in leukocytes. J. Clin. Microbiol. 33:2607-2611.