A Conserved Histidine Residue of Escherichia Coli Outer‐Membrane Phospholipase A is Important for Activity

European Journal of Biochemistry

Volumn 234, Issue 3, 1995, Pages 934-938

  Brok, Ronald G P M ^a   Dekker, Nick ^a   Gerrits, Nathalie ^a   Verheij, Hubertus M ^a   Tommassen, Jan ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

active site; Escherichia coli.; outer membrane protein; phospholipase A; site directed mutagenesis

Indexed keywords

PHOSPHOLIPASE A;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; BLOTTING, WESTERN; CELL MEMBRANE; CONSERVED SEQUENCE; DNA PRIMERS; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HISTIDINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOLIPASES A; PROTEIN FOLDING; TEMPERATURE;

EID: 0029618716     PISSN: 00142956     EISSN: 14321033     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1995.934_a.x     Document Type: Article

References (36)

1. Studies on lysophospholipases VIT: Synthesis of acylthioester analogs of lysolecithin and their use in a continuous spectrophotometric assay for lysophospholipases, a method with potential applicability to other lipolytic enzymes
2. Phospholipase A activity in growing Escherichia coli cells
3. On the similarity of the active‐site peptides of liver carboxylesterases
4. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
5. Band‐stab PCR: a simple technique for the purification of individual PCR products
6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding
7. The molecular evolution of genes and proteins: a tale of two serines
8. Molecular characterization of enterobacterial pldA genes encoding outer‐membrane phospholipase A
9. A monoclonal antibody against a 135‐K Golgi membrane protein
10. A role for phospholipid hydrolysis in the lysis of Escherichia coli infected with bacteriophage T4
11. Molecular cloning of pldA, the structural gene for outer‐membrane phospholipase of E. coli K12
12. Overproduction and high‐yield purification of phospholipase A from the outer membrane of Escherichia coli K‐12
13. Structure and function of lipases
14. An outer‐membrane protein (OmpA) of Escherichia coli K‐12 undergoes a conformational change during export
15. Apparent molecular masses of a heat‐modifiable protein from the outer membrane of Escherichia coli in gels with different acrylamide concentrations
16. A rapid boiling method for the preparation of bacterial plasmids
17. The DNA sequence encoding pldA gene, the structural gene for detergent‐resistant phospholipase A of E. coli
18. Kinetic characterization of Escherichia coli outer‐membrane phospholipase A using mixed detergent‐lipid micells
19. Inactivation of Escherichia coli outer‐membrane phospholipase A by the affinity label hexadecanesulfonyl fluoride. Evidence for an active‐site serine
20. The α‐subunit of mouse 75 nerve growth factor is an inactive serine protease
21. Bacterial lipases
22. Serine proteases: structure and mechanism of catalysis
23. A general method for rapid site‐directed mutagenesis using the polymerase chain reaction
24. Effects of divalent cations and of phospholipase A activity on excretion of cloacin DF13 and lysis of host cells
25. Action of organophosphates and sulfonyl halides on porcine pancreatic lipase
26. A general and fast method to generate multiple site directed mutations
27. Elevated intrinsic reactivity of seryl hydroxyl groups within the linear peptide triads His‐Xaa‐Ser or Ser‐Xaa‐His
28. Detergent‐resistant phospholipase A of Escherichia coli K‐12. Purification and properties
29. The α/β hydrolysis fold
30. Colicin E2 release: lysis, leakage or secretion? Possible role of a phospholipase
31. Role of lipopolysaccharide in assembly of Escherichia coli outer‐membrane proteins OmpA, OmpC, and OmpF
32. DNA sequencing with chain‐terminating inhibitors
33. The ultimate localization of an outer‐membrane protein of Escherichia coli K‐12 is not determined by the signal sequence
34. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors