Quantitative detection of alternatively spliced transcripts of the aromatase cytochrome P450 (CYP19) gene in aromatase-expressing human cells by competitive RT-PCR

Molecular and Cellular Probes

Volumn 9, Issue 6, 1995, Pages 453-464

  Agarwal, Veena R ^a   Bulun, Serdar E ^a   Simpson, Evan R ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Alternatively spliced transcripts; Aromatase cytochrome P450 (CYP19) gene; Competitive RT PCR; Human

Indexed keywords

EID: 0029616317     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1995.0069     Document Type: Article

References (34)

1. Mendelson, C. R., Wright, E. E., Porter, J. C., Evans, C. T. & Simpson, E. R. (1985). Preparation and characterization of polyclonal and monoclonal antibodies against human aromatase cytochrome P450 (P-450arom), and their use in its purification. Archives of Biochemistry and Biophysics 243, 480-91.
2. Nakajin, S., Shimoda, M. & Hall, P. F. (1986). Purification to homogeneity of aromatase from human placenta. Biochemical and Biophysical Research Communications 134, 704-10.
3. Kellis, J. T., Jr. & Vickery, L. E. (1987). Purification and characterization of human placental aromatase cytochrome P450. Journal of Biological Chemistry 262, 4413-20.
4. Osawa, V., Yoshida, N., Franckowiak, M. & Kitawaki, J. (1987). Immunoaffinity purification of aromatase cytochrome P450 from human placental microsomes, metabolic switching from aromatization to 1b and 2b-monohydroxylation, and recognition of aromatase isoenzymes. Steroids 50, 11-28.
5. Nebert, D. W., Nelson, D. R., Adesnik, M. et al. (1989). The P450 gene superfamily: recommended nomenclature. DNA 8, 1-13.
6. Thompson, E. A., Jr. & Siiteri, P. K. (1974). The involvement of human placental microsomal cytochrome P450 in aromatization. Journal of Biological Chemistry 249, 5373-8.
7. Akhtar, M., Calder, M. R., Corina, D. L. & Wright, J. N. (1982). Mechanistic studies on C19 demethylation in oestrogen biosynthesis. Biochemical Journal 201, 569-80.
8. Caspi, E., Wicha, J., Arunachalam, T., Nelson, P. & Spiteller, G. (1984). Estrogen biosynthesis. Concerning the obligatory intermediacy of 2b-hydroxy-10b-for-mylandrost-4-ene-3, 17-dione. Journal of the American Chemical Society 106, 7282-3.
9. Cole, P. A. & Robinson, C. H. (1988). A peroxide model reaction for placental aromatase. Journal of the American Chemical Society 110, 1284—5.
10. Goto, J. & Fishman, J. (1977). Participation of a non-enzymatic transformation in the biosynthesis of estrogens from androgens. Science 195, 80-1.
11. McNatty, K. P., Baird, D. T., Bolton, A., Chambers, P., Corker, C. S. & MacLean, H. (1976). Concentrations of oestrogens androgens in human ovarian venous plasma and follicular fluid throughout the menstrual cycle. Journal of Endocrinology 71, 77-85.
12. Fritz, I. B., Griswald, M. D., Louis, B. F. & Dorrington, J. H. (1976). Similarity of responses of cultured Sertoli cells to cholera toxin and FSH. Molecular and Cellular Endocrinology 5, 289-94.
13. Valladares, L. E. & Payne, A. H. (1979). Induction of testicular aromatization by luteinizing hormone in mature rats. Endocrinology 105, 431-6.
14. Tsai-Morris, C. H., Aquilano, D. R. & Dufau, M. L. (1984). Gonadotropic regulation of aromatase activity in the adult rat testis. Annals of the New York Academy of Sciences 438, 666-9.
15. Grodin, J. M., Siiteri, P. K. & MacDonald, P. C. (1973). Source of estrogen production in postmenopausal women. Journal of Clinical Endocrinology and Metabolism 36, 207-14.
16. Naftolin, F., Ryan, K. J., Davies, I. J. et al. (1975). The formation of estrogens by central neuroendocrine tissues. Recent Progress in Hormone Research 31, 295-319.
17. Roselli, C. E., Horton, L. E. & Resko, J. A. (1985). Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system. Endocrinology 117, 2471-4.
18. Hemsell, D. L., Grodin, J. M., Brenner, P. F., Siiteri, P. K. & MacDonald, P. C. (1974). Plasma precursors of estrogen. II. Correlation of the extent of conversion of plasma androstenedione to estrone with age. Journal of Clinical Endocrinology and Metabolism 38, 476-9.
19. Edman, C. D. & MacDonald, P. C. (1976). The role of extraglandular estrogen in women in health and disease. In The Endocrine Function of the Human Ovary (James, V. H. T., Serio, M. & Ciusti, C. eds). Pp. 135-40. London: Academic Press.
20. MacDonald, P. C. (1978). Origin of estrogen in postmenopausal women at increased risk of endometrial carcinoma. In Hormonal Biology of Endometrial Cancer (Richardson, G. S. & MacLaughlin, D. T. eds). Pp. 15-35. U.I.C.C. Technical Report Series.
21. Bulun, S. E., Price, T. M., Mahendroo, M. S., Aitken, J. & Simpson, E. R. (1993). A link between breast cancer and local estrogen biosynthesis suggested by quantification of breast adipose tissue aromatase cytochrome P450 transcripts using competitive polymerase chain reaction after reverse transcription. Journal of Clinical Endocrinology and Metabolism 77, 1622-8.
22. Simpson, E. R., Mahendroo, M. S., Means, G. D. et al. (1994). Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis. Endocrine Reviews 15, 342-55.
23. Mahendroo, M. S., Mendelson, C. R. & Simpson, E. R. (1993). Tissue-specific and hormonally-controlled alternative promoters regulate aromatase cytochrome P450 gene expression in human adipose tissue. Journal of Biological Chemistry 268, 19463-70.
24. Corbin, C. J., Graham-Lorence, S., McPhaul, M., Mason, J. I., Mendelson, C. R. & Simpson, E. R. (1988). Isolation of a full length cDNA insert encoding human aromatase system cytochrome P-450 and its expression in non-steroidogenic cells. Proceedings of the National Academy of Sciences of the USA 85, 8948-52.
25. Sarkar, G. & Bolander, M. E. (1994). The "looped oligo" method for generating reference molecules for quantitative PCR. BioTechniques 17, 864-6.
26. Chirgwin, J. M., Przybyla, A. E., MacDonald, R. J. & Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribo-nuclease. Biochemistry 18, 5294-9.
27. Mendelson, C. R., Evans, C. T. & Simpson, E. R. (1987). Regulation of aromatase in estrogen-producing tissues. Journal of Steroid Biochemistry 27, 753-7.
28. Ackerman, G. E., Smith, M. E., Mendelson, C. R., MacDonald, P. C. & Simpson, E. R. (1981). Aroma-tization of androstenedione by human adipose tissue stromal cells in monolayer culture. Journal of Clinical Endocrinology and Metabolism 53, 412-1 7.
29. McAllister, J. M., Mason, J. I., Byrd, W., Trant, J. M., Waterman, M. R. & Simpson, E. R. (1990). Proliferating human granulosa-lutein cells in long-term monolayer culture: expression of aromatase, cholesterol side chain cleavage and 3b-hydroxysteroid dehydrogenase. Journal of Clinical Endocrinology and Metabolism 71, 26-33.
30. Mullis, K. B. (1991). The polymerase chain reaction in an anemic mode: how to avoid cold oligo-deoxyribonuclear fusion. PCR Methods and Applications 1, 1-4.
31. Erlich, H. A., Gelfand, D. & Sninsky, J. J. (1991). Recent advances in the polymerase chain reaction. Science 252, 1643-51.
32. Simpson, E. R., Ackerman, G. E., Smith, M. E. & Mendelson, C. R. (1981). Estrogen formation in stromal cells of adipose tissue of women: induction by gluco-corticosteroids. Proceedings of the National Academy of Sciences of the USA 78, 5690-4.
33. Zhao, Y., Nichols, J. E., Bulun, S. E., Mendelson, C. R. & Simpson, E. R. (1995). Aromatase P450 gene expression in human adipose tissue: role of a Jak/STAT pathway in regulation of the adipose-specific promoter. Journal of Biological Chemistry270, 16449-57.
34. Jenkins, C., Michael, D., Mahendroo, M. & Simpson, E. (1993). Exon-specific northern analysis and rapid amplification of cDNA ends (RACE) reveal that the proximal promoter II (Pll) is responsible for aromatase cytochrome P450 (CYP19) expression in human ovary. Molecular and Cellular Endocrinology 97, R1-R6.