A Drosophila homolog of the yeast origin recognition complex

Science

Volumn 270, Issue 5242, 1995, Pages 1674-1677

  Gossen, Manfred ^a   Pak, Daniel T S ^a   Hansen, Stig K ^a   Acharya, Jairaj K ^b   Botchan, Michael R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN; POLYPEPTIDE;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CELL NUCLEUS; DNA REPLICATION ORIGIN; DROSOPHILA MELANOGASTER; EMBRYO; EMBRYO DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

EID: 0029597854     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.270.5242.1674     Document Type: Article

References (30)

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12. The Drosophila genomic DNA that potentially encodes a protein with sequence similarity to yeast Orc2p was identified in the course of analyzing the nearby IPP gene. A P-element insertion in close proximity to the IPP gene was rescued, and the genomic DNA was further subcloned as Eco Rl fragments. The homology to yeast Orc2p was identified by sequencing the insert boundaries of two of these subclones, which were later identified as sequences flanking the Eco Rl site indicated within the DmORC2 ORF (Fig. 1A).
13. 5 plaques were screened with the same hybridization probes as described above. Thirty clones remained after three rounds of plaque purification, of which 10 were characterized. All phage clones contained sequences that matched the ORF of clone 2-2.
14. 5 plaques were screened with the same hybridization probes as described above. Thirty clones remained after three rounds of plaque purification, of which 10 were characterized. All phage clones contained sequences that matched the ORF of clone 2-2.
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30. We thank C. Zuker for providing the DmORC2 genomic clone, D. Rio for bringing the initial finding of the Zuker lab to our attention, M. Levine (University of California, San Diego) for the plasmid cDNA library, T. Kornberg for the λgt10 cDNA library, D. Rio and R. Tjian for encouragement and technical advice, and A. Ho for help. Supported by a pilot project grant from the National Institute of Environmental Health Sciences Mutagenesis Center (ES-01896), National Cancer Institute grant CA30490, and a Human Frontier Science Program Long-Term Research Fellow-ship (M.G.). Sequences of the DmORC2 and DmORCS cDNAs have been deposited in GenBank (accession numbers pending).