The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase

Glycobiology

Volumn 5, Issue 8, 1995, Pages 783-789

  Azadi, Parastoo ^a   O’ Neill, Malcolm A ^a   Bergmann, Carl ^a   Darvill, Alan G ^a   Albersheim, Peter ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

Pectin; Rhamnogalacturonan; Rhamnogalacturonase; Rhamnogalacturonolyase

Indexed keywords

GLUCAN SYNTHASE; PECTIN;

AMPEROMETRY; CARBOHYDRATE ANALYSIS; CELL WALL; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; GAS CHROMATOGRAPHY; GEL CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PLANT CELL; PRIORITY JOURNAL; TECHNIQUE; ULTRAVIOLET SPECTROSCOPY;

CARBOHYDRATE SEQUENCE; CELL WALL; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSIDE HYDROLASES; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR SEQUENCE DATA; PECTINS; POLYSACCHARIDE-LYASES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TREES;

ASPERGILLUS ACULEATUS;

EID: 0029591249     PISSN: 09596658     EISSN: 14602423     Source Type: Journal    
DOI: 10.1093/glycob/5.8.783     Document Type: Article

References (23)

1. Albersheim, P. (1966) Pectin lyase from fungi. Methods Enzymol., 8. 629-631.
2. An, J., O’Neill, M.A., Albersheim, P. and Darvill, A.G. (1994a) Isolation and structural characterization of P-D-glucosyluronic acid and 4-O-melhy! {i-D-glucosyluronic acid-containing oligosaccharides from the cell-wall pectic polysaccharide, rhamnogalacturonan I. Carbohydr. Res., 252, 235-243.
3. An, J., O’Neill, M.A., Darvill, A.G. and Albersheim, P. (1994b) Isolation and structural characterization of endo-rhamnogalacturonase-generated fragments of the backbone of rhamnogalacturonan I. Carbohydr. Res., 264, 83-96.
4. Carpita, JM.C. and Gibeaut, D.M. (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the wall during growth. Plant J., 3, 1-30.
5. Colquhoun, IJ., deRuiter, G.A., Schols, H.A., Beldman, G. and Voragen, A.GJ. (1990) Identification by n.m.r. spectroscopy of oligosaccharides obtained by treatment of the hairy regions of apple pectin with rhamnogalacturonase. Carbohydr. Res., 206, 131-144.
6. Darvill, A.G., McNeil, M., Albersheim, P. and Delmer, P. (1980) The primary cell walls of higher plants. In Tolbert, N.E. (ed.), The Biochemistry of Plants. Academic Press, New York, Vol. 1, pp. 91-162.
7. Davis, K.R., Darvill, A.G., Albersheim, P. and Dell, A (1986) Host-pathogen interactions. XXIX. Oligogalacturonides released from sodium polypectate by endopolygalacturomc acid lyase are elicitors of phytoalexins in soybean. Plant Physiol., 80, 568-577.
8. Ishii, T., Thomas, J R., Darvill, A.G., and Albersheim, P. (1989) The structure of plant cell walls XXVI. The walls of suspension-cultured sycamore cells contain a family of rhamnogalacturonan-I-like pectic polysaccharides. Plant Physiol., 89, 421-428.
9. Kofod, L.V., Kauppinen, S., Christgau, S., Anderson, L.N., Heldt-Hansen, H.P, Dorreich, K. and Dalboge, H. (1994) Cloning and characterization of two structural and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. J. Biol. Chem., 269, 29182-29189.
10. Lau, J.M., McNeil, M., Darvill, A.G. and Albersheim, P. (1985) Structure of the backbone of rhamnogalacturonan I, a pectic polysaccharide in the cell walls of plants. Carbohydr. Res., 137, 111-125.
11. Lau, J.M., McNeil, M., Darvill, A.G. and Albersheim, P. (1987) Treatment of rhamnogalacturonan I with lithium in ethylenediamine. Carbohydr. Res., 168, 245-274.
12. Lerouge, P., O’Neill, M.A., Darvill, A.G. and Albersheim, P. (1993) Structural characterization of endo-glycanase-generated oligoglycosyl side chains of rhamnogalacturonan I. Carbohydr. Res., 243, 359-371.
13. Matheson, N.K. and McCleary, B.V. (1995) Enzymes metabolizing polysaccharides and their application to the analysis of structure and function of glycans In Aspinall.G.O. (ed.), The Polysaccharides. Academic Press, New York, Vol. 3, pp. 1-105.
14. McNed, M., Darvill, A.G. and Albersheim, P. (1980) Structure of plant cell walls. X. Rhamnogalacturonan I, a structurally complex pectic polysaccharide in the walls of suspension-cultured sycamore cells. Plant Physiol., 66, 1128-1134.
15. McNeil, M., Darvill, A.G. and Albersheim, P. (1982) Structure of plant cell walls. XII. Identification of seven differently linked glycosyl residues attached to 0-4 of the 2, 4-linked L-rhamnosyl residues of rhamnogalacturonan I. Plant Physiol., 70, 1586-1591.
16. ONetll, M.A., Albersheim, P. and Darvill, A.G. (1990) The pectic polysaccharides of primary cell walls. In Dey.P.M. (ed.), The Biochemistry of Plants. Academic Press, London, Vol. 2, pp. 415-441.
17. Sakamoto, T and Sakai, T. (1994) Protopectinase-T: a rhamnogalacturonase able to solubilize protopectin from sugar beet pectin. Carbohydr. Res., 259, 77-91.
18. Schols, H.A. and Voragen, A.G.J. (1994) Occurrence of pectic hairy regions in various plant cell wall materials and their degradability by rhamnogalacturonase. Carbohydr. Res., 256, 83-95.
19. Schols, H.A., Gereads, C.CJ.M., Searlr-van Leeuwen, M.F., Kormelink, FJ.M. and Voragen, A.G.J. (1990) Rhamnogalacturonase: a novel enzyme that degrades the hairy regions of pectins. Carbohydr. Res., 206, 105-115.
20. Spiro, M.D., Kates, K.A., Koller, A.L., O’Neill, M.A., Albersheim, P. and Darvill, A.G. (1993) Purification and characterization of biologically active 1, 4-linked a-D-oligogalacturonides after partial digestion of polygalacturonic acid with endopolygalacturonase. Carbohydr. Res., 247, 9-20.
21. Thomas, J.R., Darvill, A.G. and Albersheim, P. (1989) Rhamnogalacturonan I, a pectic polysaccharide that is a component of monocot cell walls. Carbohydr. Res., 185, 279-305.
22. Tjan, S.B., Voragen, A.GJ. and Pilmik, W. (1974) Analysis of some partly and fully esterified oligogalactopyranuronic acids by P.M.R. spectrometry at 220 MHz. Carbohydr. Res., 34, 15-23.
23. York, W.S., Darvill, A.G., McNeil, M., Stevenson, T.T. and Albersheim, P. (1985) Isolation and characterization of plant cell walls and cell wall constituents. Methods Enzymol., 118, 1-45.