Sodium-driven potassium uptake by the plant potassium transporter HKT1 and mutations conferring salt tolerance

Science

Volumn 270, Issue 5242, 1995, Pages 1660-1663

  Rubio, Francisco ^b   Gassmann, Walter ^b   Schroeder, Julian I ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; POTASSIUM ION; SODIUM CHLORIDE;

ARTICLE; HIGHER PLANT; MICHAELIS CONSTANT; NONHUMAN; PHYTOTOXICITY; POINT MUTATION; PRIORITY JOURNAL;

EID: 0029583478     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.270.5242.1660     Document Type: Article

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16. +-starved cells [J. Ramos and A. Rodríguez-Navarro, Eur. J. Biochem. 154, 307 (1986)] were analyzed as described (28).
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21. + uptake was determined at pH 6 and 8, no differences in initial uptake rates were observed (n = 3). In oocytes, HKT1-mediated outward currents are evident in the absence of external substrate (see Fig. 2) (11). The effects of pH on reversal potentials in oocytes (11) were obtained by subtraction of HKT1-mediated pre-steady-state outward currents from each other, which gave rise to apparent pH effects (11).
22. + were added as glutamate salts.
23. W. Gassmann, F. Rubio, J. I. Schroeder, data not shown.
24. +.
25. +]. The HKT1 cDNAs of these 10 plasmids were sequenced to identify mutations. The cDNA of the HKT1 mutants was subcloned under the control of the yeast PMA1 gene promoter, as described (13).
26. F. Rubio, W. Gassmann, J. I. Schroeder, data not shown.
27. Abbreviations for the amino acid residues are as follows: A, Ala; F, Phe; L, Leu; and V, Val.
28. -1 (L247F).
29. W. Gassmann, F. Rubio, J. I. Schroeder, data not shown.
30. -1).
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35. We thank R. F. Gaber and A. Rodríguez-Navarro for providing yeast strains, W. B. Frommer for providing the plasmid pDR195 and A. Rodríguez-Navarro, N. M. Crawford, A. Schinder, and J. M. Ward for comments. Supported by U.S. Department of Agriculture (95-37304-2227) and Department of Energy (DE-FG03-34-ER20148) grants and a Presidential Young Investigator Award (to J.I.S.) and a Ministerio de Educación y Ciencia (Spain) postdoctoral fellowship (to F.R.).