Expanded bed affinity chromatography of dehydrogenases from bakers' yeast using dye–ligand perfluoropolymer supports

Biotechnology and Bioengineering

Volumn 48, Issue 4, 1995, Pages 341-354

  McCreath, Graham E ^a   Chase, Howard A ^a   Owen, Ryan O ^a   Lowe, Christopher R ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

affinity chromatography; dehydrogenases; dye ligand; expanded bed; perfluoropolymers

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELLS; DYES; EMULSIONS; ENZYME IMMOBILIZATION; ENZYME KINETICS; FLUORINE CONTAINING POLYMERS; PACKED BEDS; PURIFICATION; YEAST;

BIOSEPARATION; DEHYDROGENASES; DYE-LIGAND PERFLUOROPOLYMER SUPPORTS; ENZYME ACTIVITY; EXPANDED BED AFFINITY CHROMATOGRAPHY; GLUCOSE 6-PHOSPHATE DEHYDROGENASE; MALATE DEHYDROGENASE; SELECTIVE ELUTION;

ENZYMES;

FUNGAL PROTEIN; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; MALATE DEHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

AFFINITY CHROMATOGRAPHY; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; NONHUMAN; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0029394842     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.260480407     Document Type: Article

References (39)

1. Protein purification by dye‐ligand chromatography
2. Design of novel cationic ligands for the purification of trypsin‐like proteases by affinity chromatography
3. Microfiltration membranes as pseudo‐affinity adsorbents: Modification and comparison with gel beads
4. Dye‐ligand membranes as selective adsorbents for rapid purification of enzymes: A case study
5. Novel technologies for direct extraction of proteins
6. Purification of proteins by adsorption chromatography in expanded beds
7. Affinity purification of proteins using expanded beds
8. High‐performance affinity chromatography
9. Monosized adsorbents for high performance affinity chromatography. Application to the purification of calf intestinal alkaline phosphatase and human urine urokinase
10. Process scale high‐performance liquid affinity chromatography
11. The purification of glucose 6‐phosphate dehydrogenase by dye‐ligand chromatography
12. Assessment of the suitability of commercially available SpA affinity solid phases for the purification of murine monoclonal antibodies at process scale
13. The malate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, characterization and studies on their regulation
14. Single‐step recovery of a secreted recombinant protein by expanded bed adsorption
15. Hydrophobic and other non‐ionic parameters in protein separation and adsorptive immobilization by substituted agaroses
16. Aqueous 2‐phase systems in biochemical recovery: Systematic analysis, design and implementation of practical processes for the recovery of proteins
17. Partial purification of D‐glucose 6‐phosphate dehydrogenase from bakers' yeast by affinity partitioning using polymer‐bound triazine dyes
18. Preparative chromatography of biomolecules
19. Purification of recombinant protein A by aqueous two‐phase extraction integrated with affinity separation
20. Handbook of biochemistry and molecular biology
21. Bioseparation media and modes
22. Purification procedure and N‐terminal amino acid sequence of yeast malate, dehydrogenase isoenzymes
23. Interaction of yeast glucose 6‐phosphate dehydrogenase with diverse triazine dyes: A study by means of affinity partitioning
24. Zonal chromatographic analysis of the interaction of alcohol dehydrokenase with blue sepharose
25. Designer dyes: “Biomimetic” ligands for the purification of pharmaceutical proteins by affinity chromatography
26. Applications of triazinyl dyes in protein purification
27. Novel affinity separations based on perfluorocarbon emulsions. Use of a perfluorocarbon affinity emulsion for the direct extraction of glucose‐6‐phosphate dehydrogenase from homogenized bakers' yeast
28. Novel affinity separations based on perfluorocarbon emulsions. Use of a perfluorocarbon affinity emulsion for the purification of human serum albumin from blood plasma in a fluidized bed
29. Novel affinity separations based on perfluorocarbon emulsions. Development of a perfluorocarbon emulsion reactor for continuous affinity separations and its application in the purification or human serum albumin from blood plasma
30. Preparative affinity chromatography of proteins
31. Development and modelling of continuous affinity separations using perfluorocarbon emulsions
32. The use of immobilized triazine dyes for the purification of 6‐phosphogluconate dehydrogenase from Bacillus stearothermophilus
33. Evidence that yeast cell wall debris can separate proteins by ion‐exchange during cell‐lysis
34. Affinity chromatography on triazine dyes immobilized on novel perfluorocarbon supports
35. Affinity chromatography on novel perfluorocarbon supports. Immobilization of C. I. Reactive Blue 2 on a polyvinyl alcohol‐coated perfluoropolymer support and its application in affinity chromatography
36. Development of an enzyme linked immunosorbent assay for CI Reactive Blue 2 and its application to a comparison of the stability and performance of a perfluorocarbon support with other immobilized CI Reactive Blue 2 affinity adsorbents
37. A process for large‐scale isolation of β‐galactosidase from E. coli in an aqueous two‐phase system
38. Production, purification and characterization of recombinant human interferon δ