Development of an expanded bed technique for an affinity purification of G6PDH from unclarified yeast cell homogenates

Biotechnology and Bioengineering

Volumn 48, Issue 4, 1995, Pages 355-366

  Chang, Y K ^a,b   McCreath, G E ^a   Chaset, H A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b MING CHI UNIVERSITY OF TECHNOLOGY   (Taiwan)

Author keywords

baker's yeast; dye ligand affinity purification; expanded beds; GGPDH; Procion Red H E7B; STREAMLINE Red H E7B

Indexed keywords

ADSORBENTS; ADSORPTION; CELLS; DYES; ENZYMES; ETHANOL; PACKED BEDS; PH; PURIFICATION; SODIUM COMPOUNDS; UREA; YEAST;

AFFINITY PURIFICATION; BAKER YEAST; CLEANING-IN-PLACE PROCEDURES; DYE-LIGAND AFFINITY PURIFICATION; ELUENTS; ELUTION; EXPANDED BEDS; PURIFICATION FACTOR; YEAST CELL HOMOGENATES;

AFFINITY CHROMATOGRAPHY;

GLUCOSE 6 PHOSPHATE DEHYDROGENASE;

CELL FREE SYSTEM; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; NONHUMAN; YEAST;

EID: 0029394832     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.260480408     Document Type: Article

References (32)

1. Purification of recombinant ricin A chain with immobilized triazine dyes
2. Purification by exploitation of activity
3. A novel ion‐exchange method for the isolation of streptomycin
4. Development of a recovery process for novobiocin
5. Effects of ligand concentration on protein adsorption in dye ligand adsorbents
6. Batch fluidized ion exchange column for streams containing suspended particles
7. Continuous affinity chromatography using a magnetically stabilized fluidized bed
8. Dye‐ligand membranes as selective adsorbents for rapid purification of enzymes: A case study
9. Expanded bed adsorption for the direct extraction of proteins
10. Novel technologies for direct extraction of proteins
11. Prediction of the performance of preparative affinity chromatography
12. Purification of proteins by adsorption chromatography in expanded beds
13. Affinity purification of proteins using expanded beds
14. Continuous protein separation in a magnetically stabilized fluidized bed using nonmagnetic supports
15. Effect of particle shape on hindered settling in creeping flow
16. Longitudinal dispersion of liquid flowing through fixed and fluidized beds
17. The purification of yeast glucose‐6‐phosphate dehydrogenase by dye‐ligand chromatography
18. Pilot scale recovery of recombinant annexin V from unclarified Escherichia coli homogenate using expanded bed adsorption
19. Fluidized bed adsorption for whole broth extraction
20. Generalized friction factor and drag coefficient correlations for fluid‐particle interactions
21. Optimization and simulation of continuous affinity recycle extraction (CARE)
22. Single‐step recovery of a secreted recombinant protein by expanded bed adsorption
23. Partial purification of D‐glucose‐6‐phosphate dehydrogenase from bakers yeast by affinity partitioning using polymer‐bound triazine dyes
24. Countercurrent distribution of yeast enzymes with polymer‐bound triazine dye affinity ligands
25. Trends and future prospects of aqueous two‐phase extraction
26. Tracer experiments in fixed beds: Effects of flow maldistribution on the estimation of transport kinetic parameters
27. Affinity chromatography on immobilized dyes
28. Novel affinity separations based on perfluorocarbon emulsions: Use of a perfluorocarbon affinity emulsion for the direct extraction of glucose‐6‐phosphate dehydrogenase from homogenised bakers' yeast
29. Preparative affinity chromatography of proteins
30. Sedimentation and fluidization: Part 1
31. Isolation and purification of endopolygalacturonase by affinity chromatography in a fluidized bed reactor
32. Purification of monoclonal antibodies from whole hybridoma fermentation broth by fluidized bed adsorption