Polyketide synthesis in vitro on a modular polyketide synthase

Chemistry and Biology

Volumn 2, Issue 9, 1995, Pages 583-589

  Wiesmann, Kirsten E H ^a   Cortés, Jesus ^a   Brown, Murray J B ^a   Cutter, Annabel L ^a   Staunton, James ^a   Leadlay, Peter F ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

cyclase; erythromycin; polyketide synthase; Saccharopolyspora erythraea; triketide lactone

Indexed keywords

HYBRID PROTEIN; MULTIENZYME COMPLEX;

ARTICLE; BIOSYNTHESIS; CELL FREE SYSTEM; CHEMISTRY; ENZYMOLOGY; METABOLISM; SACCHAROPOLYSPORA; STEREOISOMERISM;

CELL-FREE SYSTEM; MULTIENZYME COMPLEXES; RECOMBINANT FUSION PROTEINS; SACCHAROPOLYSPORA; STEREOISOMERISM;

SACCHAROPOLYSPORA; SACCHAROPOLYSPORA ERYTHRAEA; ILEX COOKII;

EID: 0029360426     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/1074-5521(95)90122-1     Document Type: Article

References (19)

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5. Complex organization of the Streptomyces avermitilis genes encoding the avermectin polyketide synthase
6. Characterization of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence
7. Stereospecific acyl transfers on the erythromycin-producing polyketide synthase
8. Identification of DEBSI, DEBS2 and DEBS3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea
9. The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin
10. Limited proteolysis and active-site studies of the first multienzyme component of the erythromycin-producing polyketide synthase
11. The thioesterase of the erythromycin-producing polyketide synthase mechanistic studies in vitro to investigate its mode of action and substrate specificity
12. Repositioning of a domain in a modular polyketide synthase to promote specific chain cleavage
13. A mutant generated by expression of an engineered DEBSI protein from the erythromycin-producing polyketide synthase (PKS) in Streptomyces coelicolor produces the triketide as a lactone but the major product is the nor-analogue derived from acetate as starter unit
14. Proton transfer in methylmalonylCoA epimerase from Propionibacterium shermanii: studies with specifically tritiated (2R)-methymalonyl-CoA as substrate
15. An acyl-carrier protein-thioesterase domain from the 6-deoxyerythronolide B synthase of Saccharopolyspora erythraea. High level production, purification and characterisation in Escherichia coli
16. Mechanism of action of cerulenin on fatty acid synthetase - effect of cerulenin on iodoacetamide-induced malonyl-CoA decarboxylase activity
17. Engineered biosynthesis of a triketide lactone from an incomplete modular polyketide synthase
18. Heterologous expression in Escherichia coli of an intact multienzyme component of the erythromycin-producing polyketide synthase
19. Adenosylcobalamin-dependent methylmalonyl-CoA mutase from Propionibacterium shermanii: active holoenzyme produced in Escherichia coli