Rapid optimization of electroporation conditions for plant cells, protoplasts, and pollen

Molecular Biotechnology

Volumn 3, Issue 3, 1995, Pages 181-190

  Saunders, James A ^a   Lin, Chin Ho ^b   Hou, Bi Huei ^b   Cheng, Jianping ^a   Tsengwa, Nombasa ^c   Lin, Jhy Jhu ^d   Smith, Camelia R ^a   McIntosh, Maria S ^c   Van Wert1, Sally ^a,e  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^c UNIVERSITY OF MARYLAND   (United States)

^d Life Technologies Inc   (United States)

^e AgrEvo USA Company   (United States)

Author keywords

Electroporation; gene transfer; Medicago; Nicotiana

Indexed keywords

ARTICLE; ELECTROPORATION; GENE TRANSFER; GENETIC PROCEDURES; GENETICS; PLANT; POLLEN; PROTOPLAST;

ELECTROPORATION; GENE TRANSFER TECHNIQUES; GENETIC TECHNIQUES; PLANTS; POLLEN; PROTOPLASTS;

EID: 0029316039     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/BF02789328     Document Type: Article

References (22)

1. Recent advances in plant electroporation
2. Review: Electroporation in biology, methods, applications and instrumentation
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6. Gene transfer in plants
7. Relationship between protoplasts size and critical field strength in protoplast electropulsing and application to reliable DNA uptake
8. Uptake and metabolism of clomazone in tolerant-soybean and susceptible-cotton photomixophic cell suspension cultures
9. A revised medium for rapid growth and bioassays with tobacco tissue culture
10. Rapid starch synthesis associated with increased respiration in germinating lily pollen
11. Reduction of nuclease activity released from germinating pollen under conditions used for pollen electrotransformation
12. The evaluation of pollen quality and a further appraisal of the fluorochromatic (FCR) test procedure
13. Behavior and viability of tobacco protoplasts in response to electrofusion parameters
14. Determination of pollen viability in tomatoes
15. Transient gene expression in intact and organized rice tissue
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17. Gene transfer into intact plant cells by electroinjection through cell walls and membranes
18. The permeability of electroporated cells and protoplasts of sugar beet
19. DNA uptake during electroporation of germinating pollen grains
20. Fusion of mammalian cells in culture is obtained by creating the contact between cells after their electropermeabilization
21. Electrofusion and electroporation of plants