A dual-specificity pseudouridine synthase: An Escherichia coli synthase purified and cloned on the basis of its specificity for Φ746 in 23S RNA is also specific for Φ32 in tRNAphe

RNA

Volumn 1, Issue 4, 1995, Pages 437-448

  Wrzesinski, Jan ^a   Nurse, Kelvin ^a   Bakin, Andrey ^a   Lane, Byron G ^b   Ofengand, James ^a  

^a HOFFMANN LA ROCHE INC   (United States)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Chemical RNA sequencing; Large subunit rRNA; Modified nucleosides; Overexpression; Polyhistidine tag; RNA modifying enzyme

Indexed keywords

ISOMERASE; MAGNESIUM; MUTASE; PHENYLALANINE TRANSFER RNA; PSEUDOURIDINE; PSEUDOURIDINE 746 32 SYNTHASE; PSEUDOURIDINE 746-32 SYNTHASE; RECOMBINANT PROTEIN; RNA 23S;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BIOSYNTHESIS; COMPARATIVE STUDY; DRUG EFFECT; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RNA PROCESSING; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; INTRAMOLECULAR TRANSFERASES; ISOMERASES; MAGNESIUM; MOLECULAR SEQUENCE DATA; PSEUDOURIDINE; RECOMBINANT PROTEINS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, RIBOSOMAL, 23S; RNA, TRANSFER, PHE; SEQUENCE ANALYSIS, RNA; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0029313081     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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