A universally conserved region of the largest subunit participates in the active site of RNA polymerase III

EMBO Journal

Volumn 14, Issue 15, 1995, Pages 3766-3776

  Dieci, Giorgio ^a,b   Denmat, Sylvie Hermann Le ^a,c   Lukhtanov, Evgeny ^a,d   Thuriaux, Pierre ^a   Werner, Michel ^a   Sentenac, André ^a  

^a CEA SACLAY   (France)

^b UNIVERSITY OF PARMA   (Italy)

^c ECOLE NORMALE SUPÉRIEURE   (France)

^d Nanogen Inc USA   (United States)

Author keywords

Pausing; RNA polymerase III; Slippage; Transcription; Yeast; ' subunit

Indexed keywords

FUNGAL PROTEIN; MUTANT PROTEIN; POLY(DEOXYADENYLATE DEOXYTHYMIDYLATE); RNA POLYMERASE II; TRANSFER RNA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; FUNGUS GROWTH; LETHAL MUTANT; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RNA GENE; RNA TRANSCRIPTION; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; YEAST;

BACTERIA (MICROORGANISMS); EUKARYOTA; FUNGI;

EID: 0029157035     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1002/j.1460-2075.1995.tb00046.x     Document Type: Article

References (45)

1. Allison,L.A., Moyle,M., Shales,M. and Ingles,C.J. (1985) Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell, 42, 599-610.
2. Boeke,J.D., Lacroute,F. and Fink,G.R. (1984) A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet., 197, 345-346.
3. Bonner,G., Lafer,E.M. and Sousa,R. (1994) Characterization of a set of T7 RNA polymerasc active site mutants. J. Biol. Chem., 269, 25120-25128.
4. BorukhovS., Lee,J. and Goldfarb,A. (1991) Mapping of a contact for the RNA 3′ terminus in the largest subunit of RNA polymerase. J. Biol. Chem., 266, 23932-23935.
5. Borukhov,S., Sagitov,V., Josaitis,C A., Gourse,R.L. and Goldfarb,A. (1993) Two modes of transcription initiation in vitro at the rrnB P1 promoter of Escherichia coli. J. Biol.Chem., 268, 23477-23482.
6. Burgess,R.R. (1969) Separation and characterization of the subunits of ribonucleic acid polymerase. J. Biol. Chem., 244, 6168-6176.
7. Chamberlin,M.J. (1994) New models for the mechanism of transcription elongation and its regulation. Harvey Lecture, Series 88, Wiley-Liss, New York, pp. 1-21.
8. Chiannilkulchai,N., Moenne,A., Sentenac,A. and Mann,C. (1992) Biochemical and genetic dissection of the Saccharomyces cerevisiae RNA polymerase C53 subunit through the analysis of a mitochondrially mis-sorted mutant construct. J. Biol. Chem., 267, 23099-23107.
9. Coulter,D.F. and Greenleaf,A.L. (1985) A mutation in the largest subunit of RNA polymerase II alters RNA chain elongation in vitro. J. Biol. Chem., 260, 13190-13198.
10. Darst,S.A., Kubalek,E.W. and Kornberg,R.D (1989) Three-dimensional structure of Escherichia coli RNA polymerase holoenzyme determined by electron microscopy. Nature, 340, 730-731.
11. Darst,S.A., Edwards,A.M., Kubalek,E.W. and Kornberg,R.D. (1991) Three-dimensional structure of yeast RNA polymerase II at 16 Å resolution. Cell, 66, 121-128.
12. de Mercoyrol,L., Job,C. and Job,D. (1989) Studies on the inhibition by α-amanitin of single-step addition reactions and productive RNA synthesis catalyzed by wheat germ RNA polymerase II. Biochem. J., 258, 165-169.
13. Gabrielsen,O.D., Marzouki,N., Ruet,A., Sentenac,A. and Fromageot,P. (1989) Two polypeptide chains in yeast transcription factor t interact with DNA. J. Biol. Chem., 264, 7505-7511.
14. Grachev,M.A., Luchtanov,E.A., Mustaev,A.A., Zaychikov,E.F., Abdukayumov,M.N., Rabinov,I.V. Richter.V.I., Skoblov,Y.S. and Christyakov,P.G. (1989) Studies on the functional topography of Escherichia coli RNA polymerase. A method for localization of the sites of affinity labeling. Eur. J. Biochem., 180, 577-585.
15. Hermann-Le Denmat,S., Werner,M., Sentenac,A. and Thuriaux,P. (1994) Suppression of yeast RNA polymerase III mutations by FHLI, a gene coding for a fork head protein involved in rRNA processing. Mol. Cell. Biol., 14, 2905-2913.
16. Huet,J., Riva,M., Sentenac,A. and Fromageot,P. (1985) Yeast RNA polymerase C and its subunits: specific antibodies as structural and functional probes. J. Biol. Chem., 260, 15304-15310.
17. m for purinc nucleotidcs during transcription elongation. J. Biol. Chem., 266, 14478-14485.
18. Jin,D.J. and Turnbough,C.L. Jr (1994) An Escherichia coli RNA polymerase defective in transcription due to its overproduction of abortive initiation products. J. Mol. Biol., 236, 72-80.
19. Joyce,C.M. and Steitz,T.A. (1994) Function and structure relationships in DNA polymerases. Annu. Rev. Biochem., 63, 777-822.
20. Kashlev,M., Lee,J., Zalenskaya,K., Nikiforov,V. and Goldfarb,A. (1990) Blocking the initiation-to-elongation transition by a transdominant RNA polymerase mutation. Science, 248, 1006-1009.
21. Kassavetis,G.A. and Chamberlin,M.J. (1981) Pausing and termination of transcription within the early region of bacteriophage T7 DNA in vitro. J. Biol. Chem., 256, 2777-2786.
22. Kassavetis,G.A., Riggs,D.L., Negri,R., Nguyen,L.H. and Geiduschek,E.P. (1989) Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes. Mol. Cell. Biol., 9, 2551-2566.
23. Landick,R., Stewart,J. and Lee,D.N. (1990) Amino acid changes in conserved regions of the β-subunit of Escherichia coli RNA polymerase alter transcription pausing and termination. Genes Dev., 4, 1623-1636.
24. Lee,J., Kashlev,M., Borukhov,S. and Goldfarh,A. (1991) A β subunit mutation disrupting the catalytic function of Escherichia coli RNA polymerase. Proc. Natl Acad. Sci. USA, 88, 6018-6022.
25. Martin,C.T., Muller,D.K. and Coleman,J.E. (1988) Processivity in early stages of transcription by T7 RNA polymerase. Biochemistry, 27, 3966-3974.
26. Matsuzaki,H., Kassavetis,G.A. and Geiduschek,E.P. (1994) Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III. J. Mol. Biol., 235, 1173-1192.
27. Mémet,S., Gouy,M., Marck,C., Sentenac,A. and Buhler,J.M. (1988) RPA190, the gene coding for the largest subunit of yeast RNA polymerase A. J. Biol. Chem., 263, 2830-2839.
28. Moenne,A., Camier,S., Anderson,G., Margottin,F., Beggs,J. and Sentenac,A. (1990) The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro. EMBO J., 9, 271-277.
29. Mustaev,A., Kashlev,M., Lee,J., Polyakov,A., Lebedev,A., Zalenskaya,K., Grachev,M., Goldfarb,A. and Nikiforov,V. (1991) Mapping the priming substrate contacts in the active center of Escherichia coli RNA polymerase. J. Biol. Chem., 266, 23927-23931.
30. Nudler,E., Goldfarb,A. and Kashlev,M. (1994) Discontinuous mechanism of transcription elongation. Science, 265, 793-796.
31. Parker,R.C. (1986) Synthesis of in vitro ColE1 transcripts wilh 5'-terminal ribonucleotides that exhibit noncomplementarity with the DNA template. Biochemistry, 25, 6593-6598.
32. Riva,M., Carles,C., Sentenac,A., Grachev,M.A., Mustaev,A.A. and Zaychikov,E.F. (1990) Mapping the active site of yeast RNA polymerase B (II). J. Biol. Chem., 265, 16498-16503.
33. Sagitov,V., Nikiforov,V. and Goldfarb,A. (1993) Dominant lethal mutations near the 5' substrate binding site affect RNA polymerase propagation. J. Biol. Chem., 268, 2195-2202.
34. Scafe,C. Martin,C., Nonet,M., Podos,S., Okamura,S. and Young,R. (1990) Conditional mutations occur predominantly in highly conserved residues of RNA polymerase II suhunits. Mol. Cell. Biol.. 10, 1270-1275.
35. Schultz,P., Célia,H., Riva,M. Sentenac,A. and Oudet,P. (1993) Three-dimensional model of RNA polymerase I determined by electron microscopy of two-dimensional crystals. EMBO J., 12, 2601-2607.
36. 1 promoter. J. Biol. Chem., 269, 31701-31705.
37. Shaaban,S.A., Krupp,B.M. and Hall,B.J. (1995) Termination-altering mutations in the second-largest subunit of yeast RNA polymerase III. Mol. Cell. Biol., 15, 1467-1478.
38. Steinberg,T.H. and Burgess,R.R. (1992) Tagetitoxin inhibition of RNA polymerase III transcription results from enhanced pausing at discrete sites and is template-dependent. J. Biol. Chem., 267, 20204-20211.
39. Sweetser,D., Nonet,M. and Young,R.A. (1987) Prokaryotic and eukaryotic RNA polymerases have homologous core suhunits Proc. Natl Acad. Sci. USA, 84, 1192-1196.
40. Thuillier,V., Stettler,S., Sentenuc,A., Thuriaux,P. and Werner,M. (1995) A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation. EMBO J., 14, 351-359.
41. Thuriaux,P. and Sentenac,A. (1992) Yeast nuclear RNA polymerases. In Jones,E.W., Pringle,J.R. and Broach,J.R. (eds), The Molecular Biology of the Yeast Saccharomyces cererisiae: Gene Expression. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, vol. 2, pp. 1-48.
42. Weilbaecher,R., Hebron,C., Feng,G. and Landick,R. (1994) Termination-altering amino acid substitutions in the β' subunit of Escherichia coli RNA polymerase identify regions involved in RNA chain elongation. Genes Dev., 8, 2913-2927.
43. Werner,M., Hermann-Le Denmat,S., Treich,I., Sentenac,A. and Thuriaux,P. (1992) Effect of mutations in a zinc binding domain of yeast RNA polymerase C on enzyme function and subunit association. Mol. Cell. Biol., 12, 1087-1095.
44. Whitehall,K., Bardeleben,C. and Kassavetis,G.A. (1994) Hydrolytic cleavage of nascent RNA in RNA polymerase III ternary transcription complexes. J. Biol. Chem., 269, 2299-2306.
45. Yager,T.D. and von Hippel,P.H. (1987) Transcript elongation and termination in Escherichia coli. In Neidhardt,F.C. (ed.), Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. American Society for Microbiology, Washington, DC, pp. 1242-1275.