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3
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0020181690
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Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole
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(1982)
Cell
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Stevens1
Esmon2
Schekman3
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4
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0025823035
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The Sec18/NSF protein is required at multiple steps in the secretory pathway: mutants define compartmental organization of protein modification and sorting events in the yeast Golgi
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(1991)
J Cell Biol
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Graham1
Emr2
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6
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0027297956
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Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment
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Vida1
Huyer2
Emr3
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8
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0023739386
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Protein sorting in Saccharomyces cerevisiae: isolation of mutants defective in the delivery and processing of multiple vacuolar hydrolases
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(1988)
Mol Cell Biol
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Robinson1
Klionsky2
Banta3
Emr4
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9
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0022898326
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Protein sorting in yeast: mutants defective in vacuole biogenesis mislocalize vacuolar proteins into the late secretory pathway
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Cell
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Rothman1
Stevens2
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10
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0024445121
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Characterization of genes required for protein sorting and vacuolar function in the yeast Saccharomyces cerevisiae
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(1989)
EMBO J
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Rothman1
Howald2
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11
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0017600514
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Proteinase mutants of Saccharomyces cerevisiae
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Genetics
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Jones1
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12
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0029618201
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Receptor-mediated protein sorting to the vacuole in yeast: roles for a protein kinase, a lipid kinase and GTP-binding proteins
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of special interest, This comprehensive review details the roles of a protein kinase, lipid kinase and GTP-binding proteins in vacuolar protein sorting.
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(1995)
Annu Rev Cell Dev Biol
, vol.11
, pp. 1-33
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Stack1
Horazdovsky2
Emr3
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15
-
-
0028332917
-
The sorting receptor for yeast vacuolar carboxypeptidase Y is encoded by the VPS10 gene
-
of outstanding interest, The authors identify a carboxypeptidase Y (CPY) sorting receptor, Vps10p, and show the CPY-specific sorting defect of vps10 mutant strains. Subcellular fractionation and analysis of Vps10p carbohydrate modification are used to identify the late Golgi as the site of Vps10p localization. Chemical cross-linking studies show that Vps10p and the Golgi-modified precursor form of CPY interact and this interaction is dependent on an intact CPY vacuolar sorting signal. A model for Vps10p function in vacuolar protein sorting is presented.
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(1994)
Cell
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Marcusson1
Horazdovsky2
Lin Cereghino3
Gharakhanian4
Emr5
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16
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0027067882
-
Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases
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(1992)
Mol Biol Cell
, vol.3
, pp. 415-427
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Paravicini1
Horazdovsky2
Emr3
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17
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0024095176
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Organelle assembly in yeast: characterization of yeast mutants defective in vacuolar biogenesis and protein sorting
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(1988)
J Cell Biol
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Banta1
Robinson2
Klionsky3
Emr4
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18
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0025200830
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Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae
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(1990)
Mol Cell Biol
, vol.10
, pp. 6742-6754
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Herman1
Emr2
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19
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0026098352
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A novel protein kinase homolog essential for protein sorting to the yeast lysosme-like vacuole
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(1991)
Cell
, vol.64
, pp. 425-437
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Herman1
Stack2
DeModena3
Emr4
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20
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-
0027256130
-
A membrane-associated complex containing the Vps15 protein kinase and the Vps34 PI 3-kinase is essential for protein sorting to the yeast lysosome-like vacuole
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(1993)
EMBO J
, vol.12
, pp. 2195-2204
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Stack1
Herman2
Schu3
Emr4
-
24
-
-
0028110018
-
Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities
-
of special interest, This study shows that Vps34p exhibits both a phosphatidylinositol-specific phosphoinositide (PI) 3-kinase activity, as well as a protein kinase activity that results in autophosphorylation of Vps34p. Mutations altering conserved residues in the lipid kinase domain inactivated both the PI 3-kinase and protein kinase activity of Vps34p. Unlike the mammalian p110/p85 PI 3-kinase, Vps34p is highly resistant to the inhibitor wortmannin.
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(1994)
J Biol Chem
, vol.269
, pp. 31552-31562
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Stack1
Emr2
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29
-
-
0028964232
-
Vesicle-mediated protein transport: regulatory interactions between the Vps15 protein kinase and the Vps34 Ptdlns 3-kinase essential for vacuolar protein sorting in yeast
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ts strains reveal that inactivation of Vps15p or Vps34p results both in a decline of cellular phosphatidylinositol 3-phosphate [Ptdlns(3)P] levels and in the missorting of vacuolar hydrolases. These results imply a direct role for Ptdlns(3)P in protein sorting. The authors also show that kinase-defective forms of Vps34p result in a dominant-negative phenotype whereas kinase-defective forms of Vps15p do not. In addition, chemical cross-linking studies show that Vps15p kinase mutants are defective for association with Vps34p. This indicates that an active Vps15p kinase is required for recruitment of Vps34p to the membrane.
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(1995)
J Cell Biol
, vol.129
, pp. 321-334
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Stack1
DeWald2
Takegawa3
Emr4
-
30
-
-
0028388146
-
Characterization of a phosphatidylinositol-specific phosphoinositide 3-kinase from mammalian cells
-
of outstanding interest, A thorough biochemical analysis of a phosphatidylinositol-specific phosphoinositide (PI) 3-kinase from bovine cells. This activity from cytosol was partially purified and has a native molecular weight of 280 kDa. In addition, this mammalian enzyme is resistant to the drug wortmannin, unaffected by adenosine treatment, and not activated by tyrosine-phosphorylated peptides. The authors suggest that this phosphatidylinositol-specific PI 3-kinase may have a fundamentally different role in mammalian cells than the broad-specificity PI 3-kinases.
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(1994)
Curr Biol
, vol.4
, pp. 203-214
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-
Stephens1
Cooke2
Walters3
Jackson4
Volina5
Gout6
Waterfield7
Hawkins8
-
31
-
-
0028169625
-
A phosphatidylinositol 3-kinase is induced during soybean nodule organogenesis and is associated with membrane proliferation
-
of special interest, Two phosphatidylinositol (Ptdlns) 3-kinases from soybean were cloned and characterized. The predicted sequence of these proteins shares approximately 40% sequence identity with yeast Vps34p. Expression of the root form and the nodule form of Ptdlns 3-kinase was documented in different tissues and during nodule organogenesis.
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(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 9617-9621
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-
Hong1
Verma2
-
32
-
-
0027942615
-
AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana is an essential protein with homology to a calcium-dependent lipid binding domain
-
of special interest, A phosphatidylinositol 3-kinase from Arabidopsis thaliana was cloned and characterized (40% identity to Vps34p). When antisense constructs of AtVps34 were expressed at high levels, second generation transgenic A. thaliana exhibited severe defects in growth and development.
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(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 11398-11402
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Welters1
Takegawa2
Emr3
Chrispeels4
-
34
-
-
0027997975
-
+-ATPase-defective yeast: identification of six new end genes
-
+-ATPase). Two of the end mutants (end12=vps34; end13=vps4) were identified previously as having a role in vacuolar protein sorting. Four new mutants show altered uptake of the α-factor mating pheromone.
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(1994)
J Cell Biol
, vol.127
, pp. 373-386
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Munn1
Riezman2
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35
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-
0025376380
-
A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting
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(1990)
Cell
, vol.61
, pp. 1063-1074
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Rothman1
Raymond2
Gilbert3
O'Hara4
Stevens5
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37
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0025006964
-
Molecular cloning of the microtubule-associated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins
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(1990)
Nature
, vol.347
, pp. 256-261
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Obar1
Collins2
Hammarback3
Shpetner4
Vallee5
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39
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0025810110
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Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic
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(1991)
Nature
, vol.351
, pp. 411-414
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Van der Bliek1
Meyerowitz2
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41
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0028898261
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Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding
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(1995)
Nature
, vol.374
, pp. 190-192
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Hinshaw1
Schmid2
-
42
-
-
0028953080
-
Golgi and vacuolar proteins reach the vacuole in vps1 mutant yeast cells via the plasma membrane
-
of special interest, These authors examine the localization of a late Golgi hybrid membrane protein and a vacuolar membrane protein in vps1 mutant cells that also contain mutations that disrupt membrane flow to or from the plasma membrane (sec4 and end4). The study indicates that resident late-Golgi proteins and vacuolar proteins are delivered to the vacuole via the plasma membrane in vps1 mutant cells. A model is presented in which Vps1p function is required for the formation of transport vesicles at a late-Golgi compartment.
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(1995)
J Cell Biol
, vol.129
, pp. 35-46
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Nothwehr1
Conibear2
Stevens3
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43
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-
0027186301
-
Vps1p, a member of the dynamin GTPase family, is necessary for Golgi membrane protein retention in Saccharomyces cerevisiae
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(1993)
EMBO J
, vol.12
, pp. 3049-3059
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Wilsbach1
Payne2
-
45
-
-
0028889155
-
The Saccharomyces cerevisiae MVP1 gene interacts with VPS1 and is required for vacuolar protein sorting
-
(1995)
Mol Cell Biol
, vol.15
, pp. 1671-1678
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Ekena1
Stevens2
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46
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0026555196
-
Molecular dissection of the secretory pathway
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(1992)
Nature
, vol.355
, pp. 409-415
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Rothman1
Orci2
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47
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0028385277
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Implications of the SNARE hypothesis for intracellular membrane topology and dynamics
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(1994)
Curr Biol
, vol.4
, pp. 220-233
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-
Rothman1
Warren2
-
49
-
-
0028348688
-
VPS21 encodes a rab5-like GTP binding protein that is required for the sorting of yeast vacuolar proteins
-
of special interest, This paper describes the characterization of a small GTP-binding protein required for vacuolar protein sorting. Mutational analysis demonstrates that conserved GTP-binding, effector and prenylation domains found in Vps21p are required for function. In addition, small 40–50 nm vesicles accumulate in vps21 cells.
-
(1994)
EMBO J
, vol.13
, pp. 1297-1309
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Horazdovsky1
Busch2
Emr3
-
50
-
-
0028181704
-
Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast
-
of special interest, Using a PCR based approach, two homologues of mammalian rab5, YPT51 and YPT53, were identified in yeast. Another rab5 homologue, YPT52, was found on chromosome XI as part of the yeast genome sequencing initiative. One of these homologues, YPT51, was found to be the same gene as VPS21. In addition to vacuolar protein sorting defects, ypt51 mutants show a defect in the vacuolar delivery of endocytic markers. These phenotypes were exaggerated in ypt51 ypt52 and ypt51 ypt52 ypt53 double and triple mutants, indicating a role for these proteins in the late endocytic and vacuolar protein localization pathways.
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(1994)
The Journal of Cell Biology
, vol.125
, pp. 283-298
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Singer-Kruger1
Stenmark2
Dusterhoft3
Philippsen4
Yoo5
Gallwitz6
Zerial7
-
51
-
-
0026011490
-
Mutational analysis of the putative effector domain of the GTP-binding Ypt1 protein in yeast suggests specific regulation by a novel GAP activity
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(1991)
EMBO J
, vol.10
, pp. 785-792
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Becker1
Tan2
Trepte3
Gallwitz4
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52
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0024276923
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A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast
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(1988)
Cell
, vol.53
, pp. 753-768
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Goud1
Salminen2
Walworth3
Novick4
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53
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0027092937
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Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)
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(1992)
Cell
, vol.71
, pp. 1131-1142
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Wichmann1
Hengst2
Gallwitz3
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54
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0027449288
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Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast
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(1994)
J Cell Sci
, vol.106
, pp. 823-830
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Schimmöller1
Riezman2
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55
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0028605474
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Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar protein sorting defects and accumulation of membrane vesicles
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(1994)
J Cell Sci
, vol.107
, pp. 3449-3459
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Cowles1
Emr2
Horazdovsky3
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56
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0028595960
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Yeast Vsp45p is a Sec1p-like protein required for the consumption of vacuole-targeted, post-Golgi transport vesicles
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(1994)
Eur J Cell Biol
, vol.65
, pp. 305-318
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Piper1
Whitters2
Stevens3
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57
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0025178838
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Characterization of yeast Vps33p, a protein required for vacuolar protein sorting and vacuole biogenesis
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(1990)
Mol Cell Biol
, vol.10
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Banta1
Vida2
Herman3
Emr4
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59
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0027364502
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Synaptic vesicle fusion complex contains unc-18 homologue bound to syntaxin
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(1993)
Nature
, vol.366
, pp. 347-351
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Hata1
Slaughter2
Südhof3
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64
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0026652588
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A role for clathrin in the sorting of vacuolar proteins in the Golgi complex of yeast
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(1992)
EMBO J
, vol.11
, pp. 2811-2818
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Seeger1
Payne2
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65
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0024447838
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Membrane protein sorting: biosynthesis, transport and processing of yeast vacuolar alkaline phosphatase.
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(1989)
EMBO J
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, pp. 2241-2250
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Klionsky1
Emr2
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66
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0025255677
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A new class of lysosomal/vacuolar protein sorting signals
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(1990)
J Biol Chem
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Klionsky1
Emr2
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67
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0026727566
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Membrane protein sorting in the yeast secretory pathway: evidence that the vacuole may be the default compartment
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J Cell Biol
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, pp. 69-83
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Roberts1
Nothwehr2
Stevens3
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68
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0027080272
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Mutation of a tyrosine localization signal in the cytosolic tail of yeast Kex2 protease disrupts Golgi retention and results in default transport to the vacuole
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(1992)
Mol Biol Cell
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, pp. 1353-1371
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Wilcox1
Redding2
Wright3
Fuller4
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70
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0027085824
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Yeast Kex1p is a Golgi-associated membrane protein — deletions in a cytoplasmic domain result in the mislocalization to the vacuolar membrane
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(1992)
J Cell Biol
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, pp. 1459-1468
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Cooper1
Bussey2
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71
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0028283489
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Sorting of membrane proteins in the yeast secretory pathway
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of special interest, A comprehensive review that describes vacuolar membrane protein localization, as well as retention and recycling of resident membrane proteins in the secretory and vacuolar protein localization pathways.
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(1994)
J Biol Chem
, vol.269
, pp. 10185-10188
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Nothwehr1
Stevens2
|