A proposed mechanism of ADP-ribosylation catalyzed by the pertussis toxin S1 subunit

Biochimie

Volumn 77, Issue 5, 1995, Pages 333-340

  Locht, C ^a   Antoine, R ^a  

^a INSTITUT PASTEUR DE LILLE   (France)

Author keywords

catalytic residues; enzyme mechanism; NAD glycohydrolase; pertussis toxin

Indexed keywords

ADENOSINE DIPHOSPHATE RIBOSE; CYSTEINE; GLUTAMIC ACID DERIVATIVE; GUANINE NUCLEOTIDE BINDING PROTEIN; HISTIDINE; NICOTINAMIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE; PERTUSSIS TOXIN; PROTEIN SUBUNIT;

ADENOSINE DIPHOSPHATE RIBOSYLATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONFERENCE PAPER; ENZYME BINDING; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; NONHUMAN; NUCLEOTIDE METABOLISM; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ADENOSINE DIPHOSPHATE RIBOSE; AMINO ACID SEQUENCE; ANIMAL; BACTERIAL TOXINS; BINDING SITES; BORDETELLA PERTUSSIS; GTP-BINDING PROTEINS; NAD; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T; VIRULENCE FACTORS, BORDETELLA;

EID: 0029147581     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/0300-9084(96)88143-0     Document Type: Article

References (56)

1. Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1
2. Mono-ADP-ribosyltransferases and ADP-ribosylarginine hydrolases: a mono-ADP ribosylation cycle in animal cells
3. Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution
4. Crystal structure of a cholera toxin-related heat-labile enterotoxin from E coli
5. The crystal structure of diphtheria toxin
6. The crystal structure of pertussis toxin
7. Active-site mutations of diphtheria toxin: effects of replacing glutamic acid-148 with aspartic acid, glutamine, or serine
8. Pseudomonas aeruginosa exotoxin A: alterations of biological and biochemical properties resulting from mutation of glutamic acid 553 to aspartic acid
9. Evidence for a catalytic role of glutamic acid 129 in the NAD-glycohydrolase activity of the pertussis toxin S1 subunit
10. Activation by thiol of the latent NAD glycohydrolase and ADP-ribosyltransferase activities of Bordetella pertussis toxin (islet activating protein)
11. Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model
12. Pertussis toxin gene: nucleotide sequence and genetic organization
13. Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication
14. Site-specific alterations of the B oligomer that affect receptor-binding activities and mitogenicity of pertussis toxin
15. The multiple biological activities of pertussis toxin
16. G proteins identified as pertussis toxin substrates
17. Pertussis toxin-catalyzed ADP-ribosylation of transducin: cysteine 347 is the ADP-ribose acceptor site
18. Biological activities of pertussigen (pertussis toxin)
19. Two guanine nucleotide-binding proteins in rat brain serving as the specific substrate of islet-activating protein, pertussis toxin. Interaction of the α-subunits with βγ-subunits in development of their biological activities
20. Peptide inhibitors of ADP-ribosylation by pertussis toxin are substrates with affinities comparable to those of the trimeric GTP-binding proteins
21. Role of cysteine 41 of the A subunit of pertussis toxin
22. Roles of the disulfide bond and the carboxy-terminal region of the S1 subunit in the assembly and biosynthesis of pertussis toxin
23. Adenine nucleotides promote dissociation of pertussis toxin subunits
24. The role of cysteine 41 in the enzymatic activities of the pertussis toxin S1 subunit as investigated by site-directed mutagenesis
25. The carboxyl terminus of the S1 subunit of pertussis toxin confers high affinity binding to transducin
26. Enzymatic activity of cholera toxin. I. New method of assay and the mechanism of ADP-ribosyl transfer
27. Identification of a region in the S1 subunit of pertussis toxin that is required for enzymatic activity and that contributes to the formation of a neutralizing antigenic determinant
28. Subunit S1 of pertussis toxin: mapping of the regions essential for ADP-ribosyltransferase activity
29. The NAD-glycohydrolase activity of the pertussis toxin S1 subunit: involvement of the catalytic His-35 residue
30. + to pertussis toxin
31. Identification of amino acid residues essential for the enzymatic activities of pertussis toxin
32. Probing the structure-function relationship of the pertussis toxin S1 subunit by site-directed mutagenesis
33. Role of tryptophan 26 in the NAD glycohydrolase reaction of the S-1 subunit of pertussis toxin
34. Interaction of fragment A from diphtheria toxin with nicotinamide adenine dinucleotide
35. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa
36. Pertussis toxin S1 mutant with reduced enzyme activity and a conserved protective epitope
37. Photolabelling of mutant forms of the S1 subunit of pertussis toxin with NAD
38. Identification of an active site-residue in subunit S1 of pertussis toxin by photocrosslinking to NAD
39. NAD binding site of diphtheria toxin: identification of a residue within the nicotinamide subsite by photochemical modification with NAD
40. Active site of Pseudomonas aeruginosa exotoxin A glutamic acid 553 is phospholabeled by NAD and shows functional homology with glutamic acid 148 of diphtheria toxin
41. Role of histidine 35 of the S1 subunit of pertussis toxin in the ADP-ribosylation of transducin
42. Inactivation of the Escherichia coli heat-labile enterotoxin by in vitro mutagenesis of the A-subunit gene
43. Glutamic acid-112 of the A subunit of heat-labile enterotoxin from enterotoxinogenic Escherichia coli is important for ADP-ribosyltransferase activity
44. Histidine 21 does not play a major role in diphtheria toxin catalysis
45. Active-site mutations of the diphtheria toxin catalytic domain: role of histidine 21 in nicotinamide adenine dinucleotide binding and ADP-ribosylation of elongation factor 2
46. ADP-ribosylation of elongation factor 2 by diphtheria toxin. NMR spectra and proposed structures of ribosyldiphthamide and its hydrolysis products
47. The RNA N-glycosidase activity of ricin A-chain: the characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA
48. X-ray analysis of substrate analogs in the ricin A-chain active site
49. Mutations affecting the activity of the shiga-like toxin 1 A-chain
50. Diphtheria toxin: site and configuration of ADP-ribosylation of diphthamide in elongation factor 2
51. + with guanidines
52. Pyridine nucleotide chemistry. A new mechanism for the hydroxyde-catalyzed hydrolysis of the nicotinamide-glycosyl bond
53. Photoaffinity labeling of diphtheria toxin fragment A with NAD: structure of the photoproduct at position 148
54. The stereochemistry of calf spleen NAD-glycohydrolase-catalyzed NAD methanolysis
55. Mutants of pertussis toxin suitable for vaccine development