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Angewandte Chemie International Edition in English
Volumn 34, Issue 3, 1995, Pages 301-303
Efficient Heterogeneous Biocatalysts by Entrapment of Lipases in Hydrophobic Sol–Gel Materials
Author keywords

enzymatic catalysis; immobilization; lipases; solgel processes
Indexed keywords

EID: 0029134278     PISSN: 05700833     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.199503011     Document Type: Article
Times cited : (267)
References (32)
  • 13
    • 84985512907 scopus 로고
    • “Immobilisierte Lipasen in hydrophoben Sol‐Gel‐Materialien”:, (Max‐Planck‐Institut für Kohlenforschung), DE‐A 4408152.9
    • (1994)
    • Reetz, M.T.1    Zonta, A.2    Simpelkamp, J.3
  • 26
    • 84985534229 scopus 로고    scopus 로고
    • The lipase was dissolved in water or buffer, centrifuged to remove undissolved solids, and mixed with aqueous solutions of polyvinyl alcohol and sodium fluoride. The silanes were then added in the order of increasing reactivity. The reaction mixture was thoroughly mixed and shaken until gelation occurred (ca. 1 min). The gels thus obtained were allowed to stand in sealed vessels for 24 h, dried for 3 days at 37 °C, washed with water, acetone, and pentane, dried and ground into a powder.
  • 27
    • 84985496465 scopus 로고    scopus 로고
    • We thank Novo Nordisk A/S (Denmark) for a sample of SP 523 (NOVO) and Amano Enzyme Europe Ltd. (England) for PS lipase.
  • 28
    • 84985534239 scopus 로고    scopus 로고
    • Whereas the commercial enzyme powder combined with the water produced from the reaction and formed a viscous residue which was difficult to separate, the sol–gel immobilized lipase could be recycled without any difficulty.
  • 29
    • 84985496451 scopus 로고    scopus 로고
    • We thank Dr. B. Tesche (Fritz Haber Institut, Berlin) for the scanning electron micrographs.
  • 31
    • 84985569293 scopus 로고    scopus 로고
    • According to Cesti et. al. [15] the reaction resulted in 95% ee. However, it is known that the enantioselectivity of enzyme‐catalyzed reactions can vary for different batchesm of enzyme.
  • 32
    • 84985599498 scopus 로고    scopus 로고
    • The influence of other parameters, for example the use of additives, the stoichiometry water/silane, the amount of catalyst, and the use of other silane monomers are presently under investigation in our group.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.