Pyrimidine regulation of the escherichia coli and salmonella typhimurium carab operons: CarP and integration host factor (IHF) modulate the methylation status of a GATC site present in the control region

Journal of Molecular Biology

Volumn 250, Issue 4, 1995, Pages 383-391

  Charlier, Daniel ^a,d   Gigot, Daniel ^b   Huysveld, Nadine ^c,d   Roovers, Martine ^c,d   Piérard, André ^a,b   Glansdorff, Nicolas ^a,c,d  

^a Institut de Recherches du CERIA   (Belgium)

^b UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^c VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^d Laboratorium voor Biotechnologie   (Belgium)

Author keywords

Carab; Carp; Dam methylase; Integration host factor; Pyrimidine regulation

Indexed keywords

EID: 0029119534     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1995.0384     Document Type: Editorial

References (23)

1. Birnboim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant DNA. Nucl. Acids Res. 7, 1513-1523.
2. Bouvier, J., Patte, J. C. & Stragier, P. (1984). Multiple regulatory signals in the control region of the Escherichia coli carAB operon. Proc. Natl Acad. Sci. USA, 81, 4139-4143.
3. Casadaban, M. (1976). Transposition and fusion of the lac genes to selected promoters in Escherichia coli by using bacteriophages lambda and Mu. J. Mol. Biol. 104, 541-555.
4. Charlier, D., Weyens, G., Roovers, M., Piette, J., Bocquet, C., Pierard, A. & Glansdorff, N. (1988). Molecular interactions in the control region of the carAB operon encoding Escherichia coli carbamoylphosphate synthetase. J. Mol. Biol. 204, 867-877.
5. Charlier, D., Roovers, M., Van Vliet, F., Boyen, A., Cunin, R., Nakamura, Y, Glansdorff, N. & Pierard, A. (1992). Arginine regulon of Escherichia coli K12. A study of repressor-operator interactions and of in vitro binding affinities versus in vivo repression. J. Mol. Biol. 226, 367-386.
6. Charlier, D., Roovers, M., Gigot, D., Huysveld, N., Pierard, A & Glansdorff, N. (1993). integration host factor (IHF) modulates the expression of the pyrimidine-specific promoter of the carAB operons of Escherichia coli K12 and Salmonella typhimurium LT2. Mol. Gen. Genet. 237, 273-286.
7. Charlier, D., Hassanzadeh Gh. G., Kholti, A., Gigot, D., Pierard, A. & Glansdorff, N. (1995). carP involved in pyrimidine regulation of the Escherichia coli carbamoyl-phosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA, also required for resolution of ColEI multimers. J. Mol. Biol. 250, 392-406.
8. Freundlich, M., Ramani, N., Mathew, E., Sirko, A. & Tsui, P. (1992). The role of integration host factor in gene expression in Escherichia coli. Mol. Microbiol. 6, 2557-2563.
9. Friedman, D. (1988). Integration host factor: a protein for all reasons. Cell, 55, 545-554.
10. Galas, D. J. & Schmitz, A. (1978). DNase footprinting: a simple method for the detection of protein-DNA binding specificity. Nucl. Acids Res. 5, 3157-3170.
11. Kilstrup, M., Lu, C.-D., Abdelal, A. & Neuhard, J. (1988). Nucleotide sequence of the carA gene and regulation of the carAB operon in Salmonella typhimurium. Eur. J. Biochem. 176, 421-429.
12. Lu, C.-D., Kilstrup, M., Neuhard, J. & Abdelal, A. (1989). Pyrimidine regulation of tandem promoters for carAB in Salmonella typhimurium. J. Bacteriol. 171, 5436-5442.
13. Lu, C.-D., Houghton, E. & Abdelal, A. (1992). Characterization of the arginine repressor from Salmonella typhimurium and its interaction with the carAB operator. J. Mol. Biol. 225, 11-24.
14. Miller, H. J. (1972). Experiments in Molecular Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Habor, NY.
15. Nakamaye, K. L. & Eckstein, F. (1986). Inhibition of restriction endonuclease NciI cleavage by phospho-rothioate groups and its application to oligonucleotide-directed mutagenesis. Nucl. Acids Res. 14, 9679-9698.
16. Piette, J., Nyunoya, H., Lusty, C., Cunin, R., Weyens, G., Crabeel, M., Charlier, D., Glansdorff, N. & Pierard, A. (1984). DNA sequence of the carA gene and the control region of carAB:tandem promoters, respectively controlled by arginine and the pyrimidines regulate the synthesis of carbamoylphosphate synthetase in Escherichia coli K12. Proc. Natl Acad. Sci. USA, 81, 4134-4138.
17. Roovers, M., Charlier, D., Feller, A., Gigot, D., Holemans, F., Lissens, W., Pierard, A. & Glansdorff, N. (1988). carP, a novel gene regulating the transcription of the carbamoylphosphate synthetase operon of Escherichia coli. J. Mol. Biol. 204, 857-865.
18. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl Acad. Sci. USA, 74, 5463-5469.
19. Stirling, C., Colloms, S., Collins, J., Szatmari, G. & Sherratt, D. (1989). xerB, an Escherichia coli gene required for plasmid ColEI site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine amino-peptidase. EMBO J. 8, 1623-1627.
20. Tian, G., Lim, D., Carey J. & Maas, W. K. (1992). How does the arginine repressor of Escherichia coli K12 bind to its operator sites? J. Mol. Biol. 226, 387-397.
21. Valentin-Hansen, P., Albrechtsen, B. & Love Larsen, I. (1986). DNA-protein recognition:demonstration of three genetically separated operator elements that are required for repression of the Escherichia coli deoCABD promoters by the DeoR repressor. EMBO J. 5, 2015-2021.
22. Vieira, J. & Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertional mutagenesis and sequencing with synthetic universal primers. Gene, 19, 259-268.
23. Wang, M. & Church, G. (1992). A whole genome approach to in vivo DNA-protein interactions in E. coli. Nature, 360, 606-610.