Autoantibodies to BCOADC-E2 in patients with primary biliary cirrhosis recognize a conformational epitope

Hepatology

Volumn 22, Issue 2, 1995, Pages 505-513

  Leung, Patrick S C ^a   Chuang, David T ^b   Wynn, R Max ^b   Cha, Sanghoon ^a   Danner, Dean J ^c   Ansari, Aftab ^d   Coppel, Ross L ^e   Gershwin, M Eric ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^c EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d EMORY UNIVERSITY   (United States)

^e MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

AUTOANTIBODY; EPITOPE; THIOCTIC ACID;

AMINO ACID SEQUENCE; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOBLOTTING; PRIMARY BILIARY CIRRHOSIS; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; AUTOANTIBODIES; AUTOANTIGENS; BINDING SITES; COMPARATIVE STUDY; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; HUMAN; IMMUNOSORBENT TECHNIQUES; KETONE OXIDOREDUCTASES; LIVER CIRRHOSIS, BILIARY; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SUPPORT, U.S. GOV'T, P.H.S.; THIOCTIC ACID;

EID: 0029115758     PISSN: 02709139     EISSN: None     Source Type: Journal    
DOI: 10.1016/0270-9139(95)90572-3     Document Type: Article

References (52)

1. Primary biliary cirrhosis
2. Molecular definitions, autoepitopes, and enzymatic activities of the mitochondrial autoantigens of primary biliary cirrhosis
3. Molecular aspects of the M2 autoantigens in primary biliary cirrhosis: What a difference a year makes
4. Primary biliary cirrhosis: considerations on pathogenesis based on identification of the M2 autoantigens
5. Molecular basis of mitochondrial auto-reactivity in primary biliary cirrhosis
6. Autoantibodies of primary biliary cirrhosis (PBC) recognize dihydrolipoamide acetyltransferase and inhibit enzyme function
7. Antimitochondrial antibodies of primary biliary cirrhosis recognize dihydrolipoamide acyltransferase and inhibit enzyme function of the branched chain a-keto acid dehydrogenase complex
8. Structural requirement for autoreactivity on human pyruvate dehydrogenase-E2, the major autoantigen of primary biliary cirrhosis
9. Characterization and conservation of the inner E2 core domain structure of branched-chain aketo acid dehydrogenase complex from bovine liver: construction of cDNA clone encoding the entire transacylase (E2b) precursor
10. Purification and characterization of branched chain a-keto acid dehydrogenase complex of bovine kidney
11. Construction and nucleotide sequence of a cDNA encoding the full length preprotein for human branched chain acetyltransferase
12. Subunit structure of the dihydrolipoyl transacylase component of branched chain a-keto acid dehydrogenase complex of bovine liver
13. Molecular Biology and Biochemistry of pyruvate dehydrogenase complexes
14. Domains, motifs and linkers in 2-oxo-acid dehydrogenase multienzyme complex: a paradigm in the design of a multifunctional protein
15. Expression and assembly of mature apotransacylase (E2b) of bovine branched chain a-keto acid dehydrogenase complex in Escherichia coli
16. Genetic reconstruction and characterization of the recombinant transacylase (E2b) component of bovine branched chain a-keto acid dehydrogenase complex
17. DNA sequencing with chain terminating inhibitors
18. Site-directed mutagenesis of lysine within the immunodominant autoepitope of PDC-E2
19. Molecular cloning of the liver specific rat F antigen
20. α-anions of carboxylic acids. I. Effect of hexamethylphosphoramide on metalation and alkylation
21. a-Anions of carboxylic acids. Ii. The formation and alkylation of a-methylated aliphatic acids
22. a-Anions of carboxylic acids. V. A simple high yield presentation of a-alkylhydracrylic acids and a-alkylacrylic acids
23. Primary biliary cirrhosis
24. Detection of autoantibodies to recombinant mitochondrial proteins in patients with primary biliary cirrhosis
25. Molecular characterization of the mitochondrial autoantigens in primary biliary cirrhosis
26. 2 terminus of the La/SS-B ribonucleoprotein autoantigen
27. The autoepitope of the 74kd mitochondrial autoantigen of primary biliary cirrhosis corresponds to the functional site of dihydrolipoamide acetyltransferase
28. Epitope mapping of cloned human autoantigen, histidyl tRNA synthetase
29. Epitopes of the p70 and p80 (Ku) lupus autoantigens
30. Genomic structure and amino acid sequence domains of the human La autoantigen
31. Heterologous expression and epitope mapping of a human small nuclear ribonucleoprotein associated Sm-B/B′ autoantigen
32. Mapping of multiple B cell epitopes on the 70-kilodalton autoantigen of the Ul ribonucleoprotein complex
33. Inhibitory antibody to a conformational epitope of the pyruvate dehydrogenase complex, the major antigen of primary biliary cirrhosis
34. Knowledge-based prediction of protein structures and the design of molecules
35. A nuclear antigen associated with cell proliferation and blast transformation its distribution in synchronized cells
36. PCNA/cyclin in the ciliate Euplotes eurystomus: localization in the replication band and in micronuclei
37. The presence of an antigen reactive with a human autoantibody in Trichosia pubescens (Diptera: Sciaridae) and its association with certain transcriptionally active regions of the genome
38. Gene for proliferating cell nuclear antigen (DNA polymerase sigma auxiliary protein) is present in both mammalian and higher plant genomes
39. Human autoantibody reactive epitopes of SS-B/La are highly conserved in comparison with epitopes recognized by murine monoclonal antibodies
40. Antimitochondrial antibodies in primary biliary cirrhosis recognize both specific peptide and shared epitopes of the M2 family of antigens
41. Are antimitochondrial antibodies in primary biliary cirrhosis induced by R (rough)-mutants of enterobacteriaceae?
42. Mitochondrial antibodies in primary biliary cirrhosis: species and nonspecies specific determinants of M2 antigen
43. Reactivity of primary biliary cirrhosis sera with Escherichia coli dihydrolipoamide acetyltransferase (E2p): Characterization of the main immunogenic region
44. A lipoyl synthetic octapeptide of dihydrolipoamide acetyltransferase specifically recognized by anti-M2 autoantibodies in primary biliary cirrhosis
45. Isolation and characterization of lipoylated and unlipoylated domains of the E2p subunit of the pyruvate dehydrogenase complex of Escherichia coli
46. Octanoylation of the lipoyl domains of the pyruvate dehydrogenase complex in a lipoyl-deficient strain of Escherichia coli
47. Expression and lipoylation in Escherichia coli of the inner lipoyl domain of the E2 component of the human pyruvate dehydrogenase complex
48. Lipoylated and unlipoylated domains of human PDC-E2 as autoantigens in primary biliary cirrhosis: significance of lipoate attachment
49. Antimitochondrial autoantibodies in primary biliary cirrhosis recognize cross-reactive epitope(s) on protein X and dihydrolipoamide acetyltransferase of pyruvate dehydrogenase complex
50. Heterogeneity of combinatorial human autoantibodies against PDC-E2 and biliary epithelial cells in patients with primary biliary cirrhosis
51. Antibody to two forms of dihydrolipoamide acetyltransferase (PDC-E2) in primary biliary cirrhosis
52. Molecular mimicry in primary biliary cirrhosis: evidence for biliary epithelial expression of a molecule cross-reactive with pyruvate dehydrogenase complex-E2