In Vitro Folding of Escherichia Coli Outer‐Membrane Phospholipase A

European Journal of Biochemistry

Volumn 232, Issue 1, 1995, Pages 214-219

  Dekker, Niek ^a   Merck, Karin ^a   Tommassen, Jan ^a   Verheij, Hubertus M ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

expression; outer membrane phospholipase A; protein folding; purification

Indexed keywords

OUTER MEMBRANE PROTEIN; PHOSPHOLIPASE A;

ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PURIFICATION;

BACTERIAL OUTER MEMBRANE PROTEINS; ESCHERICHIA COLI; PHOSPHOLIPASES; PROTEIN FOLDING; SUPPORT, NON-U.S. GOV'T;

ESCHERICHIA COLI;

EID: 0029101182     PISSN: 00142956     EISSN: 14321033     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1995.tb20801.x     Document Type: Article

References (37)

1. Detergent‐resistant phospholipase of Escherichia coli K12
2. Properties of purified detergent‐resistant phospholipase A of Escherichia coli K12. In‐activation and protection with detergents and phospholipids
3. Kinetic characterization of Escherichia coli outer membrane phospholipase A using mixed detergent‐lipid micelles
4. Molecular cloning of pldA, the structural gene for outer membrane phospholipase of E. coli K12
5. The DNA sequence encoding pldA gene, the structural gene for detergent‐resistant phospholipase A of E. coli
6. Molecular characterization of enterobacterial pldA genes encoding outer membrane phospholipase A
7. Crystal structures explain functional properties of two E. coli porins
8. The structure of porin from Rhodobacter capsulatus at 1.8 Å resolution
9. Experiments in membrane protein crystallization
10. A role for phosphoiipid hydrolysis in the lysis of Escherichia coli infected with bacteriophage T4
11. Atomic model of plant light‐harvesting complex by electron crystallography
12. Structure of the protein subunits in the photosynthetic reaction centre of Rhodopseudomonas viridis at 3 Å resolution
13. Model for the structure of bacteriorhodopsin based on high‐resolution electron cryo‐microscopy
14. Overproduction and high‐yield purification of phospholipase A from the outer membrane of Escherichia coli K12
15. Characteristics of detergent‐resistant phospholipase A overproduced in E. coli cells bearing its cloned structural gene
16. Use of T7 RNA polymerase to direct expression of cloned genes
17. Use of bacteriophage T7 RNA polymerase to direct selective high‐level expession of cloned genes
18. Expression using the T7 RNA polymerase/promotor system
19. Studies on lysophospholipases. VII. Synthesis of acylthioester analogs of lysolecithin and their use in a continuous spectrophotometric assay for lysophospholipases, a method with potential applicability to other lipolytic enzymes
20. Studies on transformation of Escherichia coli with plasmids
21. Periplasmic accumulation of truncated froms of outer‐membrane PhoE protein of Escherichia coli K12
22. A general and fast method to generate multiple site directed mutations
23. Electrophoretic resolution of the “major outer membrane protein” of Escherichia coli K12 into four bands
24. Architecture of the outer membrane of Escherichia coli
25. Method for determination of the amino acid sequence in peptides
26. Apparent molecular masses of a heat‐modifiable protein from the outer membrane of Escherichia coli in gels with different acrylamide concentrations
27. Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure
28. Detergent induced folding of the outer‐membrane protein PhoE, a pore protein induced by phosphate limitation
29. In vitro folding and oligomerization of a membrane protein
30. Refolding and oriented insertion of a membrane protein into a lipid bilayer
31. Refolding of an integral membrane protein
32. Assembly of an in vitro synthezised Escherichia coli outer membrane porin into its stable trimeric configuration
33. In vitro trimerization of outer membrane protein PhoE
34. 2 and lipid micelles
35. Solubilization of the overexpressed integral membrane protein alkane monooxygenase of the recombinant Escherichia coli W3110[pGEc47]