-
5
-
-
0015168319
-
Syntheses and properties of adenine and thymine nucleoside alkyl phosphotriesters, the neutral analogs of dinucleoside monophosphates
-
(1971)
J Am Chem Soc
, vol.93
, pp. 6657-6665
-
-
Miller1
Fang2
Kondo3
Ts'O4
-
9
-
-
85022440392
-
Synthesis of Oligonucleotide Analogues with Modified Backbones
-
(1993)
Synlett
, pp. 621-637
-
-
Varma1
-
11
-
-
0027730294
-
Evaluation of some properties of phosphorodithioate oligodeoxyribonucleotides for antisense application
-
of special interest, Melting temperatures, nuclease resistance, protein binding, and specific and non-specific hybridization of a phosphorodithioate-containing oligonucleotide are investigated and compared to the corresponding oligonucleotides containing phosphodiesters and phosphorothioates. The phosphorodithioate modification is reported to result in reduced protein binding, but produces higher non-specific translation inhibition and forms weaker duplexes with the complementary RNA sequence. The paper concludes that phosphorodithioated oligonucleotides have little advantage over phosphorothioate derivatives for antisense applications.
-
(1993)
Nucleic Acids Res
, vol.21
, pp. 5761-5766
-
-
Ghosh1
Ghosh2
Dahl3
Cohen4
-
15
-
-
0028136417
-
Pharmacokinetics of an antisense phosphorothioate oligodeoxynucleotide against rev from human immunodeficiency virus type 1 in the adult male rat following single injections and continuous infusion.
-
(1994)
Antisense Res Dev
, vol.4
, pp. 43-52
-
-
Iversen1
Mata2
Tracewell3
Zon4
-
16
-
-
0027503286
-
Characterization of the nuclear binding sites of oligodeoxyribonucleotides and their analogs
-
(1993)
J Biol Chem
, vol.268
, pp. 5600-5604
-
-
Clarenc1
Lebleu2
Leonetti3
-
18
-
-
0027268608
-
Cellular uptake of phosphorothioate oligodeoxynucleotides is negatively affected by the cell density in a transformed rat tracheal epithelial cell line: implication for antisense approaches
-
(1993)
Biochem Biophys Res Commun
, vol.191
, pp. 1152-1157
-
-
Iwanaga1
Ferriola2
-
19
-
-
0027433590
-
Differential distribution of oligodeoxynucleotides in developing organs with epithelial-mesenchymal interactions
-
(1993)
Nucleic Acids Res
, vol.21
, pp. 4961-4966
-
-
Rothenpieler1
Dressler2
-
25
-
-
14744277298
-
Oligonucleoside methylphosphonates as antisense reagents
-
(1991)
Biotechnology
, vol.9
, pp. 358
-
-
Miller1
-
27
-
-
0024561620
-
Use of EDTA derivatisation to characterize interactions between oligodeoxyribonucleotide methylphosphonates and nucleic acids
-
(1989)
Biochemistry
, vol.28
, pp. 1054-1061
-
-
Lin1
Blake2
Miller3
Ts'o4
-
28
-
-
0024578843
-
Effect of ionic strength on the hybridization of oligodeoxynucleotides with reduced charge due to methylphosphonate linkage to unmodified oligodeoxynucleotides containing the complementary sequence
-
(1989)
Biochemistry
, vol.28
, pp. 1040-1047
-
-
Quartin1
Wetmur2
-
33
-
-
0024826733
-
Modulation of drug resistance in a Daunorubicin resistance subline with oligonucleoside methylphosphonates
-
(1989)
Cancer Commun
, vol.1
, pp. 225-232
-
-
Vasanthakumar1
Ahmed2
-
40
-
-
0028319151
-
Hydroxymethylphosphonate: novel oligonucleotide analogue
-
(1994)
Bioorg Chem
, vol.22
, pp. 128-139
-
-
Lesnikowski1
-
41
-
-
0025777951
-
Synthesis and selective cleavage of an oligodeoxynucleotide containing a bridged internucleotide 5'-phosphorothioate linkage
-
(1991)
Nucleic Acids Res
, vol.19
, pp. 1437-1441
-
-
Mag1
Lüking2
Engels3
-
43
-
-
0025271245
-
Synthesis and properties of dithymidine phosphate analogues containing 3'-thiothymidine
-
(1990)
Nucleic Acids Res
, vol.18
, pp. 829-835
-
-
Cosstick1
Vyle2
-
45
-
-
0026471149
-
Synthesis and selective cleavage of an oligodeoxynucleotide containing bridged non-chiral internucleotide 3'-phosphoramidate linkage
-
(1992)
Tetrahedron Lett
, vol.33
, pp. 7319-7322
-
-
Mag1
Schmidt2
Engels3
-
46
-
-
0028177581
-
Oligodeoxyribonucleotide N3′→P5′ phosphoramidates: synthesis and hybridization properties
-
of special interest, Highly efficient solid phase synthesis of oligonucleotides containing 3′→P5′ phosphoramidate linkages using Atheron-Todd type coupling of a polymer-supported 5′-H-phosphonate diester with a 3′-aminonucleoside is reported. The hybridization properties of six different oligonucleotides with DNA and RNA target sequences are studied. The reported melting temperatures are impressive.
-
(1994)
J Am Chem Soc
, vol.116
, pp. 3143-3144
-
-
Gryaznov1
Chen2
-
51
-
-
0023638996
-
Uncharged stereoregular nucleic acid analogues. 1. Synthesis of a cytosine containing oligomer with carbamate internucleoside linkages
-
(1987)
J Org Chem
, vol.52
, pp. 4202-4206
-
-
Stirchak1
Summerton2
Weller3
-
52
-
-
0028344671
-
Synthesis of di-, tri- and tetrameric building blocks with novel carbamate internucleoside linkages and their incorporation into oligonucleotides
-
(1994)
Bioorg Med Chem Lett
, vol.4
, pp. 1065-1070
-
-
Habus1
Temsamani2
Agrawal3
-
60
-
-
33748217097
-
Amides as a new type of backbone modification in oligonucleotides
-
of special interest, This paper reports that replacing the backbone with amides increases both the thermodynamic stability of the duplexes formed with complementary RNA and the nuclease resistance of the antisense oligonucleotide. Conformational analysis was used to assess various low-energy conformers of the amide-modified backbones. Molecular dynamics simulations were carried out to study the dynamic behavior of the modified duplexes.
-
(1994)
Angew Chem Int Ed
, vol.33
, pp. 226-229
-
-
De Mesmaeker1
Waldner2
Lebreton3
Hoffmann4
Fritsch5
Wolf6
Freier7
-
64
-
-
0028258927
-
Oligodeoxynucleotides containing 3′-allylether, 3′-allylsulfide and their saturated derivatives as phosphate mimics
-
(1994)
Tetrahedron Lett
, vol.35
, pp. 2325-2328
-
-
Cao1
Matteucci2
-
65
-
-
0027971706
-
An approach to the structure determination of nucleic acid analogues hybridized to RNA. NMR studies of a duplex between 2′-OMe RNA and an oligonucleotide containing a single amide backbone modification
-
(1994)
Nucleic Acids Res
, vol.22
, pp. 4187-4194
-
-
Blommers1
Pieles2
De Mesmaeker3
-
67
-
-
0026683445
-
Oligonucleosides: synthesis of a novel methylhydroxylamine-linked nucleoside dimer and its incorporation into antisense sequences
-
(1992)
J Am Chem Soc
, vol.114
, pp. 4006-4007
-
-
Vasseur1
Debart2
Sanghvi3
Cook4
-
70
-
-
0027304997
-
Synthesis and binding properties of pyrimidine oligodeoxynucleoside analogs containing neutral phosphodiester replacements: the formacetal and 3′-thioformacetal internucleoside linkages
-
of special interest, The 3′-thioformacetal moiety is one of the backbone replacements for the antisense oligonucleotides that stabilize the duplex formed with a complementary RNA. Its effect on stability is compared with that of the 5′-thioformacetal and formacetal analogs.
-
(1993)
J Org Chem
, vol.58
, pp. 2983-2991
-
-
Jones1
Lin2
Milligan3
Wadwani4
Matteucci5
-
71
-
-
0028018669
-
Unusual conformation of a 3′-thioformacetal linkage in a DNA duplex
-
(1994)
J Biomol NMR
, vol.4
, pp. 17-34
-
-
Gao1
Jeffs2
-
73
-
-
0026341239
-
Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
-
(1991)
Science
, vol.254
, pp. 1497-1500
-
-
Nielsen1
Egholm2
Berg3
Buchardt4
-
76
-
-
0027364174
-
PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen bonding rules
-
of special interest, The paper describes the binding properties of mixed sequence peptide nucleic acid (PNA) to complementary DNA and RNA. It is the first demonstration that mixed sequence (as opposed to homopyrimidine sequences) PNA in fact bind to DNA and RNA with 1:1 stoichiometry; so far it is the only piece of work that has systematically assessed the thermodynamic properties of PNA-DNA and PNA-RNA duplexes and compared them to those of the corresponding DNA-DNA and DNA-RNA duplexes.
-
(1993)
Nature
, vol.365
, pp. 566-568
-
-
Egholm1
Buchardt2
Christensen3
Behrens4
Freier5
Driver6
Berg7
Kim8
Nordén9
Nielsen10
-
80
-
-
0028260073
-
DNA-like double helix formed by peptide nucleic acid
-
of special interest, This paper demonstrates that two complementary strands of PNA are actually capable of self-recognition via Watson-Crick base pairing, resulting in the formation of very stable PNA-PNA duplexes. The stability of these hybrids exceeds even that of natural DNa-DNA duplexes (see also [78]). Particularly intriguing is the finding that inherently achiral PNA-PNA duplexes can be transformed into chiral structures with a single handedness by the simple attachments of a single chiral amino acid to the end of the PNA.
-
(1994)
Nature
, vol.368
, pp. 561-563
-
-
Wittung1
Nielsen2
Buchardt3
Egholm4
Nordén5
-
85
-
-
0028066788
-
NMR solution structure of a peptide nucleic acid complexed with RNA
-
of special interest, This paper is the first report to shed light on the structure of duplexes of PNA with complementary nucleic acids (in this case RNA) at the molecular level. This work could form the basis for the design of analogs of PNA that would retain its high RNA-binding affinity, but may otherwise exhibit more favorable properties than PNA itself, for example with respect to cellular uptake.
-
(1994)
Science
, vol.265
, pp. 777-780
-
-
Brown1
Thomson2
Veal3
Davis4
-
87
-
-
0027485066
-
Single base pair mutation analysis by PNA directed PCR clamping
-
of special interest, Together with a series of other papers [81,88,89], this work illustrates the potential that peptide nucleic acids (PNA) may have for diagnostic purposes in the near future. It demonstrates that PNAs can be utilized to direct gene amplification mediated by the polymerase chain reaction in such a way as to enable the selective amplification of a specific mutant gene with only a single base pair mutation in the presence of the corresponding wild-type gene.
-
(1993)
Nucleic Acids Research
, vol.21
, pp. 5332-5336
-
-
Ørum1
Nielsen2
Egholm3
Berg4
Buchardt5
Stanley6
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