Protein analysis on a genomic scale

Journal of Biotechnology

Volumn 41, Issue 2-3, 1995, Pages 111-120

  Jungblut, Peter ^a   Wittmann Liebold, Brigitte ^a,b  

^a Wittmann Institute of Technology and Analysis of Biomolecules   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

Amino acid analysis; Electrophoresis, two dimensional; Mass spectrometry; Microsequencing; Protein database; Subtractive analysis

Indexed keywords

AMINO ACID;

AMINO ACID SEQUENCE; CONFERENCE PAPER; DATA BASE; GENOME; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; TWO DIMENSIONAL ELECTROPHORESIS;

AMINO ACIDS; ANIMAL; DATABASES, FACTUAL; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ENZYME TESTS; ENZYMES; HUMAN; IMMUNOASSAY; PROTEINS;

EID: 0029087742     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-1656(95)00006-C     Document Type: Article

References (89)

1. Electroblotting onto activated glass: High efficiency preparation of proteins from analytical sodium dodecyl sulfate polyacrylamide gels for direct sequence analysis
2. Specific antiserum staining of two-dimensional electrophoretic patterns of human plasma proteins immobilized on nitrocellulose
3. A two-dimensional gel database of human plasma proteins
4. A two-dimensional gel database of rat liver proteins useful in gene regulation and drug effect studies
5. Automatic classification of two-dimensional gel electrophoresis pictures by heuristic clustering analysis: A step toward machine learning
6. Amino acid analysis by high performance liquid chromatography and precolumn derivatisation
7. A human myocardial two-dimensional electrophoresis database: Protein characterisation by microsequencing and immunoblotting
8. Capillary electrophoresis in structural characterization of polypeptides
9. Western blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A
10. The MRC-5 human embryonal lung fibroblast two-dimensional gel cellular protein database: Quantitative identification of polypeptides whose relative abundance differs between quiescent, proliferating and SV40 transformed cells
11. The master two-dimensional gel database of human AMA cell proteins: Towards linking protein and genome sequence and mapping information
12. The human keratinocyte two-dimensional gel protein database: Update 1993
13. High resolution two-dimensional gel electrophoresis of the proteins and glycoproteins of human blood platelets and platelet membranes
14. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis
15. Differential colony hybridization: Molecular cloning from a zero database
16. Computer analysis of two-dimensional protein patterns from human heart disease
17. A genetic linkage map of the human genome
18. A new siliconized glass-fiber as support for protein-chemical analysis of electroblotted proteins
19. Identification of mouse brain proteins after two-dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysis
20. Combination of two-dimensional gel electrophoresis with microsequencing and amino acid composition analysis: Improvement of speed and sensitivity in protein characterization
21. Mass spectrometric analysis of blotted proteins after gel electrophoretic separation by matrix-assisted laser desorption/ionization
22. Structural characterization of blotting membranes and the influence of membrane parameters for electroblotting and subsequent amino acid sequence analysis of proteins
23. A protein sequenator
24. Internal protein sequence analysis: Enzymatic digestion for less than 10 μg of protein bound to polyvinylidene difluoride or nitrocellulose membranes
25. Two-dimensional gel electrophoresis and computer analysis of proteins synthesized by clonal cell lines
26. The QUEST system for quantitative analysis of two-dimensional gels
27. Transformation-sensitive and growth-related changes of protein synthesis in REF52 cells
28. Quantitative exploration of the REF 52 protein database: Cluster analysis reveals the major protein expression profiles in response to growth regulation, serum stimulation, and viral transformation
29. Identification of the phosphorylation sites of the murine small heat shock protein hsp25
30. Mouse liver protein database: A catalog of proteins detected by two-dimensional gel electrophoresis
31. Approach to stationary two-dimensional pattern: Influence of focusing time and Immobiline/carrier ampholytes concentrations
32. Plasma and red blood cell protein maps: Update 1993
33. Ueber das Carnosin eine neue organische Base des Fleischextractes
34. Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining
35. A gas-liquid phase peptide and protein sequenator
36. Phosphoamino acid analysis of protein immobilized on polyvinylidene difluoride membrane
37. Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers
38. Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by Western blotting
39. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining
40. Clinical application of high-resolution two dimensional polyacrylamide gel electrophoresis
41. Human liver protein map: Update 1993
42. Protein subunit mapping. A sensitive high resolution method
43. Two-dimensional electrophoretic analysis of proteins expressed by normal and cancerous human crypts: Application of mass spectrometry to peptide-mass fingerprinting
44. Quantitative analysis of two-dimensional electrophoretic protein patterns: Comparison of visual evaluation with computer-assisted evaluation
45. Genetic variability of proteins from mitochondria and mitochondrial fractions of mouse organs
46. Composition and genetic variability of Heparin-Sepharose CL-6B protein fractions obtained from the solubilized proteins of mouse organs
47. Composition and genetic variability of proteins from nuclear fractions of mouse (DBA/2J and C57BL/6J) liver and brain
48. Dye ligand chromatography and two-dimensional electrophoresis of complex protein extracts from mouse tissue
49. Analysis by high resolution two-dimensional electrophoresis of differentiation-dependent alterations in cytosolic protein pattern of HL-60 leukemic cells
50. Blotting efficiency investigated by using two-dimensional electrophoresis, hydrophobic membranes and proteins from different sources
51. Identification of human myocardial proteins separated by two-dimensional electrophoresis
52. Identification of tissue proteins by amino acid analysis after purification by two-dimensional electrophoresis
53. Classification of mouse liver proteins by immobilized metal affinity chromatography and two-dimensional electrophoresis
54. Protein composition of the human heart: The construction of a myocardial two-dimensional electrophoresis database
55. Ribosomal proteins. VII Two dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins
56. Protein mapping by combined isoelectric focusing and electrophoresis in mouse tissues. A novel approach to testing for induced point mutations in mammals
57. An attempt to resolve all the various proteins in a single human cell type by two-dimensional electrophoresis: I. Extraction of all cell proteins
58. High sensitivity amino acid analysis using DABS-C1 precolumn derivatization method
59. Internal amino acid sequence analysis of proteins separated by gel electrophoresis after tryptic digestion in polyacrylamide matrix
60. Differential screening of murine ascites cDNA libraries by means of in vitro transcripts of cell-cycle-phase-specific cDNA and digital image processing
61. Electrospray Its potential and limitations as an ionization method for biomolecules
62. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
63. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins
64. Capillar liquid chromatography: A tool for protein structural analysis
65. Senzitation of gas phase protein sequencer using fluorescein isothiocyanate (FITC)
66. High resolution two-dimensional electrophoresis of proteins
67. Elsie 4: Quantitative computer analysis of sets of two-dimensional gel electrophoretograms
68. Rapid identification of proteins by peptide-mass fingerprinting
69. Improved tools for biological sequence comparison
70. A two-dimensional map of human heart left ventricular proteins
71. Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes
72. Identification of transformation sensitive proteins recorded in two-dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing
73. Protein blotting: Detection of proteins with colloidal gold, and of glycoproteins and lectins with biotin-conjugated and enzyme probes
74. The amino acid sequence in the phenylalanyl chain of insulin
75. Isolation of differentially expressed genes
76. Two-dimensional gel analysis of soluble proteins. Characterization of guinea pig exocrine pancreatic proteins
77. Mass spectrometric strategies for the structural characterization of proteins
78. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some application
79. Clinical applications of two-dimensional gel electrophoresis
80. The gene-protein database of Escherichia coli: Edition 5
81. Protein-blotting on polybrene-coated glass-fiber sheets. A basis of acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on SDS-polyacrylamide gel
82. HER-MeS A second generation approach to the automatic analysis of two-dimensional electrophoresis gels Part I Data acquisition
83. Very-high resolution two-dimensional gel electrophoresis of proteins using giant gels
84. The rat liver epithelial (RLE) cell nuclear protein database
85. Tobacco mosaic virus mutants and the genetic coding problem
86. Chemical structure of bacterial ribosomal proteins
87. Die primäre Proteinstruktur von Stämmen des Tabakmosaikvirus. Aminosäuresequenzen des Proteins des TMV-Stammes dahlemense
88. Human cerebrospinal fluid protein database: Edition 1992
89. Characterization of tumor-associated protein variants during hepatocarcinogenesis in the rat