DNA duplexes containing methylated bases or non-nucleotide inserts in the recognition site are cleaved by restriction endonuclease R·EcoRII in presence of canonical substrate

Gene

Volumn 157, Issue 1-2, 1995, Pages 173-176

  Petrauskene, Olga V ^a   Gromova, Elizaveta S ^a   Romanova, Elena A ^a   Volkov, Evgeniy M ^a   Oretskaya, Tatyana S ^a   Shabarova, Zoe A ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

abasic site; activator of R EcoRII cleavage; Hemimethylated DNA duplex; modified base; recombinase; two site mechanism

Indexed keywords

4 N METHYLCYTOSINE; 6 N METHYLADENINE; DOUBLE STRANDED DNA; NUCLEIC ACID BASE; RECOMBINASE; RESTRICTION ENDONUCLEASE; UNCLASSIFIED DRUG;

CONFERENCE PAPER; DNA CLEAVAGE; DNA METHYLATION; ENZYME SUBSTRATE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

ADENINE; BASE SEQUENCE; COMPARATIVE STUDY; CYTOSINE; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; METHYLATION; MOLECULAR SEQUENCE DATA; OLIGODEOXYRIBONUCLEOTIDES; SUBSTRATE SPECIFICITY;

EID: 0029072088     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(94)00737-D     Document Type: Article

References (8)

1. Interaction of EcoRII endonuclease with DNA substrates containing single recognition sites
2. TGG sequences in DNA by restriction endonucleases MvaI and EcoRII
3. EcoRII can be activated to cleave refractory DNA recognition sites
4. Oligonucleotide duplexes containing CC(A/T)GG stimulate cleavage of refractory DNA by restriction endonuclease EcoRII
5. Mechanism of the interaction of EcoRII restriction endonuclease with two recognition sites: probing of modified DNA duplexes as activators of the enzyme
6. Two subunits of EcoRII restriction endonuclease interact with two DNA recognition sites
7. Restriction enzymes and their isoschizomers
8. Changing endonuclease EcoRII Tyr308 to Phe abolishes cleavage but not recognition: possible homology with the Int-family of recombinases