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Volumn 268, Issue 5212, 1995, Pages 836-844

Conversion of Xenopus ectoderm into neurons by neuroD, a basic helix-loop-helix protein

Author keywords

[No Author keywords available]

Indexed keywords

DIFFERENTIATION INDUCING FACTOR; HELIX LOOP HELIX PROTEIN; NEUROD; UNCLASSIFIED DRUG;

EID: 0029068316     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.7754368     Document Type: Article
Times cited : (943)

References (102)
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    • in press; J. Kirsten, unpublished data; the name of the plasmid encoding a C. elegans homolog of NeuroD is CELC34E10 and its Gene Bank accession number is U10402
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    • The whole-mount in situ hybridization was performed as described by with modifications described in (20)
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    • The antibodies were used at the following dilutions: acetylated α-tubulin (1:2000, unpublished data), Xen-1 (1:1), Xen-2 (1:1, unpublished data), and NF-M (1:2000). Primary antibodies were detected with alkaline phosphatase-conjugated goat antibody to mouse or rabbit antibodies diluted at 1:2000 (Boehringer Mannheim). Embryos stained for NF-M were fixed in Dent's solution (20 percent dimethyl sulfoxide and 80 percent methanol) and cleared in a 2:1 mixture of benzyl benzoate and benzyl alcohol
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    • In situ hybridization was performed with tanabin and β-tubulin. In situ hybridization with β-tubulin without ribonuclease treatment can also detect tubulin expression in the ciliated epidermal cells (Fig. 4, E and F)
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    • AC explants were dissected at around stage 9 and maintained in 0.5 x MBS AC explants showed ectopic N-CAM staining after 36 hours post fertilization
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    • Peng, H.B.1
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    • J. Lee and H. Weinstraub, unpublished data
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.