Frequency of UL97 Phosphotransferase Mutations Related to Ganciclovir Resistance in Clinical Cytomegalovirus Isolates

Journal of Infectious Diseases

Volumn 172, Issue 1, 1995, Pages 239-242

  Chou, Sunwen ^a,b   Guentzel, Susan ^a,b,c   Michels, Kendall R ^a,b   Miner, Richard C ^a,b   Drew, W Lawrence ^a,b  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c PORTLAND VA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; GANCICLOVIR; MUTANT PROTEIN; PHOSPHOTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; DRUG RESISTANCE; ENZYME ASSAY; HUMAN; HUMAN CYTOMEGALOVIRUS; HUMAN TISSUE; MALE; NONHUMAN; PRIORITY JOURNAL; SKIN FIBROBLAST; VIRUS MUTATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELLS, CULTURED; CODON; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA PRIMERS; DNA, VIRAL; DRUG RESISTANCE, MICROBIAL; FIBROBLASTS; GANCICLOVIR; GENES, VIRAL; HUMAN; MALE; MOLECULAR SEQUENCE DATA; PHOSPHOTRANSFERASES; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); POINT MUTATION; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; SKIN; SUPPORT, U.S. GOV'T, NON-P.H.S.;

EID: 0029061548     PISSN: 00221899     EISSN: 15376613     Source Type: Journal    
DOI: 10.1093/infdis/172.1.239     Document Type: Article

References (8)

1. Drew WL, Miner RC, Busch DF, et al. Prevalence of resistance in patients receiving ganciclovir for serious cytomegalovirus infection. J Infect Dis 1991;163:716-9.
2. Littler E, Stuart AD, Chee MS. Human cytomegalovirus UL97 open reading frame encodes a protein that phosphorylates the antiviral nucleoside analogue ganciclovir. Nature 1992;358:160-2.
3. Sullivan V, Talarico CL, Stanat SC, Davis M, Coen DM, Biron KK. A protein kinase homologue controls phosphorylation of ganciclovir in human cytomegalovirus-infected cells. Nature 1992;358:162-4.
4. Stanat SC, Reardon JE, Erice A, Jordan MC, Drew WL, Biron KK. Gan ciclovir-resistant cytomegalovirus clinical isolates: mode of resistance to ganciclovir. Antimicrob Agents Chemother 1991;35:2191-7.
5. Chou S, Erice A, Jordan MC, et al. Analysis of the UL97 phosphotransferase coding sequence in clinical cytomegalovirus isolates and identification of mutations conferring ganciclovir resistance. J Infect Dis 1995; 171:576-83.
6. Lurain N, Spafford LE, Thompson KD. Mutation in the UL97 open reading frame of human cytomegalovirus strains resistant to ganciclovir. J Virol 1994;68:4427-31.
7. Hanson MN, Preheim LC, Chou S, Talarico CL, Biron KK, Erice A. Characterization of a novel mutation in the UL97 gene of a clinical cytomegalovirus strain conferring resistance to ganciclovir. Antimicrob Agents Chemother 1995 (in press).
8. Drew WL, Miner R, Saleh E. Antiviral susceptibility testing of cytomega lovirus: criteria for detecting resistance to antivirals. Clin Diagn Virol 1993; 1:179-85.