A synthetic parvovirus B19 capsid protein can replace viral antigen in antibody-capture enzyme immunoassays

Journal of Virological Methods

Volumn 55, Issue 1, 1995, Pages 67-82

  Koch, William C ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Diagnostic test; Enzyme immunoassay; Parvovirus B19; Recombinant viral antigen

Indexed keywords

CAPSID PROTEIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; SYNTHETIC PEPTIDE; VIRUS CAPSID ANTIGEN;

ADULT; ARTICLE; CHILD; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST; ENZYME IMMUNOASSAY; HUMAN; MAJOR CLINICAL STUDY; PARVOVIRUS; PRIORITY JOURNAL; VIRUS DETECTION; VIRUS INFECTION;

ADULT; ANIMALS; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; BASE SEQUENCE; CAPSID; CAPSID PROTEINS; CELL LINE; CHILD; DNA PRIMERS; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M; MOLECULAR SEQUENCE DATA; PARVOVIRUS B19, HUMAN; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY;

INSECTA; MIRIDAE; PARVOVIRUS; UNIDENTIFIED BACULOVIRUS;

EID: 0029045583     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/0166-0934(95)00046-W     Document Type: Article

References (36)

1. Risk of human parvovirus B19 infections among school and hospital employees during endemic periods
2. Role of parvovirus B19 in human disease
3. Detection of antibodies and antigens of human parvovirus B19 by enzyme-linked immunosorbent assay
4. Human parvovirus B19 infection among hospital staff members after contact with infected patients
5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
6. Antigenic parvovirus B19 coat proteins VP1 and VP2 produced in large quantities in a baculovirus expression system
7. An immunofluorescence assay for the detection of parvovirus B19 IgG and IgM antibodies based on recombinant viral antigen
8. The role of parvovirus B19 in aplastic crisis and erythema infectiosum (fifth disease)
9. Detection of human parvovirus using a moleculary cloned probe
10. Diagnostic assays with monoclonal antibodies for the human serum parvovirus-like virus (SPLV)
11. Identification of the major structural and nonstructural proteins encoded by human parvovirus B19 and mapping of their genes by procaryotic expression of isolated genomic fragments
12. Use of recombinant human parvovirus B19 antigens in serological assays
13. A new peptide for human parvovirus antibody detection
14. Detection and purification of a recombinant human B lymphotrophic virus (HHV-6) in the baculovirus expression system by limiting dilution and DNA dot-blot hybridization
15. Occupational risk of human parvovirus B19 infection for school and day-care personnel during an outbreak of erythema infectiosum
16. A genetically engineered cell line that produces empty capsids of B19 (human) parvovirus
17. Self-assembled B19 parvovirus capsids, produced in a baculovirus system, are antigenically and immunogenically similar to native virions
18. Human parvovirus B19 infections in women of childbearing age and within families
19. Detection of human parvovirus B19 DNA by using the polymerase chain reaction
20. The use of labeled fusion protein for detection of parvovirus IgM antibodies by an immunocapture test
21. Replication of the B19 parvovirus in human bone marrow cell cultures
22. Evaluation of a commercial enzyme linked immunosorbent assay for detection of B19 parvovirus IgM and IgG
23. Parvovirus B19 outbreak in a children's ward
24. Recombinant parvovirus B19 capsids as a new substrate for detection of B19-specific IgG and IgM antibodies by an enzyme-linked immunosorbent assay
25. Cloning with PCR
26. A recombinant immunoblot and ELISA for detection of acute parvovirus B19 infections
27. Replication of parvovirus B19 in hematopoietic progenitor cells generated in vitro from normal human peripheral blood
28. Nucleotide sequence and genome organization of human parvovirus B19 isolated from the serum of a child during aplastic crisis
29. Prokaryotic expression of a VP1 polypeptide antigen for diagnosis by a human parvovirus B19 antibody enzyme immunoassay
30. Replication and cytopathology of human parvovirus B19 in human umbilical cord blood erythroid progenitor cells
31. A manual of methods for baculovirus vectors and insect cell culture procedures
32. Parvovirus B19 and human disease
33. Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver
34. Synthesis of the membrane fusion and hemagglutinin proteins of measles virus using a novel baculovirus vector containing the β-galactosidase gene
35. Use of the polymerase chain reaction for screening and evaluation of recombinant baculovirus clones