A novel assay for apoptosis Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V

Journal of Immunological Methods

Volumn 184, Issue 1, 1995, Pages 39-51

  Vermes, István ^a   Haanen, Clemens ^a   Steffens Nakken, Helga ^a   Reutellingsperger, Chris ^b  

^a MEDISCH SPECTRUM TWENTE   (Netherlands)

^b MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

Annexin V; Apoptosis; Flow cytometry; Phosphatidylserine

Indexed keywords

DEXAMETHASONE; DYE; FLUORESCEIN; LIPOCORTIN 5; PHOSPHATIDYLSERINE; PROPIDIUM IODIDE;

APOPTOSIS; ARTICLE; ASSAY; CELL KINETICS; CELL MEMBRANE; CELL SURFACE; ELECTROPHORESIS; FLOW CYTOMETRY; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ANNEXIN V; APOPTOSIS; DIPLOIDY; DNA DAMAGE; FLOW CYTOMETRY; FLUORESCEIN; FLUORESCEINS; HUMAN; MICROSCOPY; PHOSPHATIDYLSERINES; T-LYMPHOCYTES; TUMOR CELLS, CULTURED;

EID: 0029043888     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(95)00072-I     Document Type: Article

References (37)

1. Binding of vascular anticoagulant alpha (VAC alpha) to planar phospholipid bilayers
2. Apoptosis. The role of the endonuclease
3. A method to measure the duration of DNA synthesis and the potential doubling time from a single sample
4. Features of apoptotic cells measured by flow cytometry
5. Diffuse centrocytic and/or centroblasticmalignant non-Hodgkin's lymphomas: comparison of mitotic and pyknotic (apoptotic) indices
6. An improved method for the detection of DNA fragmentation
7. Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages
8. 2+-dependent membrane-binding proteins
9. Production of a mouse monoclonal antibody reactive with human nuclear antigen associated with cell proliferation
10. Review: assessment of cell proliferation in histological material
11. Human neutrophils lose their surface FcγRIII and acquire Annexine V binding sites during apoptosis in vitro
12. Flow cytometric detection of apoptosis: Comparison of the assays of in situ DNA degradation and chromatin changes
13. Annexine V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis
14. Correlations between apoptotic and proliferative indices in malignant non-Hodgkin's lymphomas
15. 2+-dependent phospholipid-binding protein
16. Pogrammed death of T cells in HIV-1 infection
17. A simple salting out procedure for extracting DNA from human nucleated cells
18. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry
19. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay
20. Lipid asymmetry in membranes
21. Quantification of apoptosis and necrosis by flow cytometry
22. A 125/115 kDa cell surface receptor specific for vitronectin interacts with the Arg-Gly-Asp adhesion sequence derived from fibronectin
23. Annexins: the problem of assessing the biological role for a gene family of multifunctional calcium- and phospholipid-binding proteins
24. Isolation and partial purification of a novel anticoagulant from arteries of human umbilical cord
25. Proportions of mitotic and apoptotic cells in a range of untreated experimental tumours
26. Phagocytic recognition of cells undergoing apoptosis
27. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry
28. Transbilayer movement of phospholipids in red cells and platelet membranes
29. Phospholipid binding properties of human placental anticoagulant protein-I, a member of the lipocortin family
30. Evaluation of glucocorticoid-induced DNA fragmentation in mouse thymocytes by flow cytometry
31. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry
32. Rapid quantitation of apoptosis in pure and heterogeneous cell populations using flow cytometry
33. Binding of Annexin V/placental anticoagulant protein to platelets
34. The assessment of proliferating cell nuclear antigen (PCNA) immuno-staining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis
35. A new method to detect apoptosis in paraffin sections
36. Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
37. Cell death: The significance of apoptosis