Use of BCECF and propidium iodide to assess membrane integrity of acutely isolated CA1 neurons from rat hippocampus

Journal of Neuroscience Methods

Volumn 58, Issue 1-2, 1995, Pages 61-75

  Bevensee, Mark O ^a   Schwiening, Christof J ^a   Boron, Walter F ^a  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

BCECF; CA1 neuron; Fluorescence; Intracellular pH; Membrane integrity; Propidium iodide; Rat hippocampus

Indexed keywords

FLUORESCENT DYE; PROPIDIUM IODIDE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN SLICE; CONTROLLED STUDY; HIPPOCAMPUS; MEMBRANE STRUCTURE; NERVE CELL; NEWBORN; NONHUMAN; PH; PRIORITY JOURNAL; RAT;

AMMONIA; ANIMAL; CELL MEMBRANE; FLUORESCEINS; FLUORESCENT DYES; HIPPOCAMPUS; HYDROGEN-ION CONCENTRATION; IN VITRO; NEURONS; OSMOLAR CONCENTRATION; PROPIDIUM; RATS; RATS, SPRAGUE-DAWLEY; SAPONINS; SODIUM; SOLUTIONS; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0029019513     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/0165-0270(94)00159-E     Document Type: Article

References (33)

1. Efflux of bis-carboxyethyl-carboxyfluorescein (BCECF) by a novel ATP-dependent transport mechanism in epithelial cells
2. Action of saponin on biological cell membranes
3. 3 and metabolic inhibitors
4. −
5. Ionic dependence of glutamate neurotoxicity
6. Glutamate neurotoxicity and diseases of the nervous system
7. Pharmacological profile of inhibition of 2′7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein efflux in human HCT-8 intestinal epithelial cells
8. Polarized efflux of 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein from cultured epithelial cell monolayers
9. Multiparametric analysis of cell membrane permeability by two colour flow cytometry with complementary fluorescent probes
10. Evidence for probenecid-sensitive organic anion transporters on polarized thyroid cells in culture
11. + exchange in platelets and leukocytes: detection by electronic cell sizing
12. Irreversible injury in anoxic hepatocytes precipitated by an abrupt increase in plasma membrane permeability
13. An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide
14. Isolation of neurons suitable for patch-clamping from adult mammalian central nervous systems
15. Measurement of cytotoxicity by target cell release and retention of the fluorescent dye bis-carboxyethyl-carboxyfluorescein (BCECF)
16. 2+ and mitochondrial membrane potential preceding cell death in hepatocytes
17. Imaging cytoskeleton-mitochondrial membrane attachments by embedment-free electron microscopy of saponin-extracted cells
18. Progressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity
19. An algorithm for least-squares estimation of nonlinear parameters
20. Preparation of mouse cell suspensions
21. 2+ concentration in single osteosarcoma cells UMR-106-01
22. Mechanisms of hydroxyl free radical-induced cellular injury and calcium overloading in alveolar macrophages
23. The neurotoxicity of excitatory amino acids is produced by passive chloride influx
24. Permeabilizing cells: some methods and applications for the study of intracellular processes
25. Fractional rate of dye signal decrease as an aid in assessing membrane permeability
26. 3 exchange
27. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ
28. 2+ overload indicates early neuronal injury which precedes staining with viability indicators
29. 2+
30. Direct observation of the agonist-specific regional vulnerability to glutamate, NMDA, and kainate neurotoxicity in organotypic hippocampal cultures