Synthesis of O-glycan core 3: Characterization of UDP-GlcNAc: GalNAc-R β3-N-acetyl-glucosaminyltransferase activity from colonic mucosal tissues and lack of the activity in human cancer cell lines

Glycobiology

Volumn 5, Issue 3, 1995, Pages 351-357

  Vavasseur, Fabienne ^a   Yang, Ji Mao ^a   Dole, Kiran ^a   Paulsen, Hans ^b   Brockhausen, Inka ^a,c  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF HAMBURG   (Germany)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

3 GlcNAc transferase; Cancer cell; Glycosyltransferase specificity; Mucin biosynthesis; O glycan core 3

Indexed keywords

N ACETYLGLUCOSAMINYLTRANSFERASE;

ARTICLE; COLON MUCOSA; ENZYME ACTIVITY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TUMOR CELL;

SUS SCROFA;

EID: 0029017524     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/5.3.351     Document Type: Article

References (29)

1. Bierhuizen M.A. and Fukudajvl F (1992) Molecular cloning of cDNA encoding UDP-GlcNAc:Gaip 1 -3GalNAc-R (GlcNAc to GalNAc) pl-6 N-acetyl- glucosaminyltransferase, an O-glycan branching enzyme, by gene transfer into CHO cells expressing polyoma large T-antigen. Pmc. Natl Acad. Sci. USA, 89, 9326-9330.
2. Brockhausen, I. (1993) Clinical aspects of glycoprotein synthesis. Crit. Rev. Clin. Lab. Sci., 30, 65-151.
3. Brockhausen, I. (1995) The biosynthesis of O-glycosylproteins. In Montreuil J., Schachter.H. and Vliegenthart J.F.G. (eds), Glycoproteins. Elsevier, Amsterdam, Holland, in press.
4. Brockhausen, I., Matta, KL., Orr J, and Schachter H. (1985) Mucin synthesis. UDP-GlcNAc:GalNAc-R P3-N-acetylglucosaminyltransferase and UDP- GlcNAc:GlcNAcpl -3 GalNAc-R (GlcNAc to GalNAc) (36-N-acetylglucos- aminyltransferase from pig and rat colon mucosa. Biochemistry, 24, 1866-1874.
5. Brockhausen, I., Moller G., Merz G., Adermann K. and Paulsen, H. (1990) Control of glycoprotein synthesis: The peptide portion of synthetic O-glycopeptide substrates influences the activity of O-glycan core 1 uridine 5'-diphospho- galactose: N-acetylgalactosaminea-R j33-galactosyl-transfera.se. Biochemistry, 29, 10206-10212.
6. Brockhausen B., Romero, PA. and Herscovics A. (1991a) Glycosyltransferase changes upon differentiation of Caco-2 human colonic adenocarcinoma cells. Cancer Res., 51, 3136-3142.
7. Brockhausen, I., Kuhns, W., Schachter H., Matta, K.L., Sutherland, D.R. and Baker, M.A. (1991b) Biosynthesis of O-glycans in leukocytes from normal donors and from patients with leukemia: increase in O-glycan core 2 UDP- GlcNAc:Gaip3GalNAca-R (GlcNAc to GalNAc) P(l-6)-N-acetylglucos- aminyltransferase in leukemic cells, Cancer Res., 51, 1257-1263.
8. Brockhausen, I., MOller G., Pollex-KrUger A., Rutz V., Paulsen, H. and Malta, K.L. (1992) Control of O-glycan synthesis in rat liver Specificity and inhibition of O-glycan core 1 UDP-Gal: GalNAca-R p3-galactosyl-transferase. Biochem Cell Biol., 70, 99-108.
9. Brockhausen, I., Vavasseur F., Yang J., Corfield A., Burchell J. and Taylor- Papadimitriou J. (1994) Regulation of glycosyltransferase activities assembling O-glycans in human cancer cells. In 3rd International Workshop on Carcinoma-associated Mucins. Imperial Cancer Research Fund, Cambridge, UK
10. Burchell J., Brockhausen, I., Winchcomb C. and Taylor-Papadimitriou, J. (1994) Mechanisms underlying the aberrant glycosylation of the MUC1 gene product observed in carcinomas. In 3rd Jenner International Glycoimmunology Meeting, Italy.
11. Capon, C., Laboisse C.L., Wieruszeski J.-M., Maorct J.-J., Augeron C. and Foumet, B. (1992) Oligosaccharide structures of mucins secreted by the human colonic cancer cell line CL.16E. J. Biol. Chem., 267, 19248-19257.
12. Gum J.R., Byrd J.C., Hicks J.W., Toribara JS.W., Lamport, D.T.A. and Kim Y.S. (1989) Molecular cloning of human intestinal mucin cDNA. Sequence analysis and evidence for genetic polymorphism. J. Biol. Chem., 264, 6480-6487.
13. King, M.-J., Chan, A., Roe, R., Warren, F., Dell, A., Morris, H.R., Bartolo C.C., Durdey P, and Corfield A.P. (1994) Two different glycosyltransferase defects that result in GalNAca-O-peptide (Tn) expression. Gtycobiology, 4, 267-279.
14. Kuhns, W., Rutz, V., Paulsen, H., Matta, KL., BakerJvf A., Bamer M., Granov- skyjvf G and Brockhausen B (1993) Processing of O-glycan core 1, Galf3l- 3GalNAca-R, in leukemia: Specificities of core 2UDP-GlcNAc: Gaipi-3GalNAc-R P6-N-acetylglucosaminyltransferase and CMP-SA: Gaipi-3GalNAc-R cG-sialyltransferase. Glycoconjugate J., 10, 381-394.
15. Meldal M., Bielfeldt, T., Peters, S., Jensen JCJ., Paulsen, H. and Bock, K (1994) Susceptibility of glycans to P-elimination in F-moc based O-glycopeptide synthesis. J. Peptide Protein Res., 43, 529-536.
16. MOller G., Reck F., Paulsen, H., Kaur K., Sarkar M., Schachter H. and Brockhausen, I. (1992) Control of glycoprotein synthesis: Properties and substrate specificity of UDP-GlcNAc: Mana3-R P2-N-acetylglucosaminyl- transferase I using synthetic substrates. Glycoconjugate J., 9, 180-190.
17. Paulsen, H., Jacquinet J.-C. and Rust, W. (1982) Synthese von Oligosaccharid- Determinanten mit Amid-Spacer vom Typ des T-antigens. Carbohydr Res., 104, 195-219.
18. Paulsen, H., Merz, G. and Brockhauscn, I. (1990) Synthese von Prolin-haltigen O-Glycopeptiden. Liebig's Ann. Chem., 719-739.
19. Paulsen, H., Rutz, V. and Brockhauscn, I. (1992) Bausteine von Oligosacchariden Oil. Synthese von modifizierten Derivalen der 2-Acetamido-2-desoxy-D- galactose zur Untersuchung der Substratspezifitat der Core-l-[33-Gal- Transferase und der Core-3-()3-GlcNAc-Transferase der Biosynthese von O-Glycoproteinen. Liebig's Ann. Chem., 735-745.
20. Paulsen, H., Peters, S., Bielfeldt T., Meldal M. and Bock, K. (1995) Synthesis of glycosyl amino acids Na-Fmoc-Ser[Ac4-D-GaIp-(l-3)-Ac2-D-GalN3p]-OPfp and N°-Fmoc-Thr[Ac4-D-Galp-(l-3)-Ac2-D-GalN3p]-OPfp and the application in the solid phase peptide synthesis of multiply glycosylated mucin peptides with Tn and T antigenic structures. Carbohydr Res., 268, 17-34.
21. Podolsky, D.K. (1985a) Oligosaccharide structures of human colonic mucin. J. Biol. Chem., 260, 8262-8271.
22. Podolsky, D.K. (1985b) Oligosaccharide structures of isolated human colonic mucin species. J. Biol. Chem., 260, 15510-15515.
23. Reck F., Meinjohanns E., Springer, M., Wilkens R., van Dorst J., Paulsen, H., Mdllcr G., Brockhausen I. and Schachter H. (1994a) Synthetic substrate analogues for UDP-GlcNAc: Manal-6R pl-2-N-acetylglucosaminyl- transferase II. Substrate specificity and inhibitors for the enzyme. Glycocon- jugate J., 11, 210-216.
24. Reckjs, R., Springer S., Paulsen, H., Brockhausen I., Sarkar M. and Schachter H. (1994b) Synthesis of tetrasaccharide analogues of the N-glycan substrate of pi-2-N-acetylglucosaminyltransferasc II using trisaccharide precursors and recombinant pi-2-N-acetylglucosaminyltransferase I. Carbohydr Res., 259, 93-101.
25. Schachter H. and Brockhausen I. (1992) The biosynthesis of serine (threonine)- N-acetylgalactosamine-linked carbohydrate moieties. In Allen, HJ.and Kisailus.E.C. (eds), Glycoconjugates. Composition, Structure and Function. Marcel Dekker, New York, pp. 263-332.
26. Vavasseur E, Yang J., Dole K., Corfield, A. and Brockhauscn, I. (1992) O-Glycan biosynthesis in human cancer cells. Glycobiology, 2, 470.
27. Vavasseur E, Dole K., Yang J., Matta K.L., Myerscough N., Corfield A., Paraskewa, C. and Brockhausen I. (1994) O-glycan biosynthesis in human colorectal adenoma cells during progression to cancer. Eur. J. Biochem., 222, 415-424.
28. Yang J., Byrd J.C., Siddiki B., Chung, Y.-S., Okuno, M., Sowa S., Kim Y.S., Matta K.L. and Brockhausen I. (1994) Alterations of O-glycan biosynthesis in human colon cancer tissues. Glycobiology, 4, 873-884.
29. Yazawa, S., Abbas, S.A., Madiyalakan R., Barlow JJ. and Matta JC.L. (1986) N-acetyl-D-glucosaminyltransferases related to the synthesis of mucin-type glycoproteins in human ovarian tissue, Carbohydr Res., 149, 241-252.