The Potential and Limitations of Neutrons, Electrons and X-Rays for Atomic Resolution Microscopy of Unstained Biological Molecules

Quarterly Reviews of Biophysics

Volumn 28, Issue 2, 1995, Pages 171-193

  Henderson, Richard ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

NITROGEN 15;

CRYSTAL; ELECTRON MICROSCOPY; FAST NEUTRON RADIATION; GEOMETRY; MOLECULAR WEIGHT; NEUTRON; OPTICAL RESOLUTION; PHASE CONTRAST MICROSCOPY; PRIORITY JOURNAL; RADIATION INJURY; REVIEW; X RAY;

BIOPHYSICS; ELECTRONS; HOLOGRAPHY; MACROMOLECULAR SYSTEMS; MICROSCOPY; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, ELECTRON; MICROSCOPY, PHASE-CONTRAST; NEUTRONS; X-RAY DIFFRACTION; X-RAYS;

EID: 0029003107     PISSN: 00335835     EISSN: 14698994     Source Type: Journal    
DOI: 10.1017/S003358350000305X     Document Type: Article

References (42)

1. ABRAMOWITZ, M. & STEGUN, I. A. (1965). Handbook of Mathematical Functions with Formulas, Graphs and Mathematical Tables. New York: Dover Publications.
2. ADRIAN, M., HEGGELER-BORDIER, B. T., WAHLI, W., STASIAK, A. Z., STASIAK, A. & DUBOCHET, J. (1990). Direct visualisation of supercoiled DNA molecules in solution. EMBO J. 9, 4551-4554.
3. ARNDT, U. (1984). Optimum X-ray wavelength for protein crystallography. J. Appl. Cryst. 17, 118–119.
4. BAKER, T. S., NEWCOMB, W. W., OLSON, N. H., COWSERT, L. M., OLSON, C. & BROWN, J. D. C. (1991). Structures of bovine and human papillomaviruses - analysis by cryoelectron microscopy and 3-dimensional image-reconstruction. Biophys. J. 60, 1445–1456.
5. BREEDLOVE, J. R. & TRAMMELL, G. T. (1970). Molecular microscopy: fundamental limitations. Science, 170, 1310–1313.
6. COWLEY, J. M. (1975). Diffraction Physics Amsterdam: North-Holland.
7. DARWIN, C. G. (1914). The theory of X-ray reflexion. Phil. Mag. 27, 315–333.
8. DE ROSIER, D. J. & KLUG, A. (1968). Reconstruction of three-dimensional structures from electron micrographs. Nature 217, 130–134.
9. ERICKSON, H. & KLUG, A. (1971). Measurement and compensation of defocusing and aberrations by Fourier processing of electron micrographs. Phil. Trans. Roy. Soc. Lond., B 261, 105–118.
10. GABOR, D. (1948). A new microscopic principle. Nature, 161, 777–778.
11. GLAESER, R. M. & CESKA, T. A. (1989). High-voltage electron diffraction from bacteriorhodopsin (purple membrane) is measurably dynamical. Acta Cryst. A45, 620–628.
12. GONZALEZ, A., THOMPSON, A. W. & NAVE, C. (1992). Cryo-protection of protein crystals in intense X-ray beams. Rev. Sci. Instrum. 63, 1177–1180.
13. HENDERSON, R. (1990). Cryo-protection of protein crystals against radiation damage in electron and X-ray diffraction. Proc. Roy. Soc. B 241, 6–8.
14. HENDERSON, R. (1992). Image contrast in high resolution electron microscopy of biological macromolecules: TMC in ice. Ultramicroscopy 46, 1–18.
15. HENDERSON, R. & GLAESER, R. M. (1985). Quantitative analysis of image contrast in electron micrographs of beam-sensitive crystals. Ultramicroscopy 16, 139–150.
16. HENDERSON, R., BALDWIN, J. M., CESKA, T. A., BECKMANN, E., ZEMLIN, F. & DOWNING, K. (1990). A model for the structure of bacteriorhodopsin based on high resolution electron cryomicroscopy. J. Mol. Biol. 213, 899–929.
17. HOPPE, W. (1983). Electron diffraction with the transmission electron microscope as a phase-determining diffractometer - from spatial frequency filtering to the three-dimensional structure analysis of ribosomes. Angew. Chem. Int. Ed. Engl. 22, 456–485.
18. HUBBELL, J. H., GIMM, H. A. & OVERBO, I. (1980). Pair, triplet and total atomic cross sections (and mass attenuation coefficients) for 1Mev-100Gev photons in elements Z = 1 to 100. J. Phys. Chem. Ref. Data 9, 1023–1147.
19. JACOBSEN, C., WILLIAMS, S., ANDERSON, E., BROWNE, M. T., BUCKLEY, C. J., KERN, D., KIRZ, J., RIVERS, M. & ZHANG, X. (1991). Diffraction-limited imaging in a scanning transmission X-ray microscope. Optics Commun. 86, 351–364.
20. JAP, B. K., WALIAN, P. J. & GEHRING, K. (1991). Structural architecture of an outer-membrane channel as determined by electron crystallography. Nature 350, 167–170.
21. JENG, T.-W., CROWTHER, R. A., STUBBS, G. & CHIU, W. (1989). Visualisation of alpha-helices in tobacco mosaic virus by cryo-electron microscopy. J. Mol. Biol. 205, 251–257.
22. KIRZ, J., ADE, H., JACOBSEN, C., KO, C.-H., LINDAAS, S., MCNULTY, I., SAYRE, D., WILLIAMS, S. & ZHANG, X. (1992). Soft X-ray microscopy with coherent X-rays. Rev. Set. Instrum. 63, 557–563.
23. KUHLBRANDT, W. & WANG, D. N. (1991). Three-dimensional structure of plant light-harvesting complex determined by electron crystallography. Nature 350, 130–134.
24. KUHLBRANDT, W., WANG, D. N. & FUJIYOSHI, Y. (1994). Atomic model of plant light-harvesting complex by electron crystallography. Nature 367,.614–621
25. LANGMORE, J. P. & SMITH, M. F. (1992). Quantitative energy-filtered electron microscopy of biological molecules in ice. Ultramicroscopy 46, 349—373.
26. LICHT, H. (1991). Optimum focus for taking electron holograms. Ultramicroscopy 38, 13–22.
27. PENCZEK, P., RADERMACHER, M. & FRANK, J. (1992). Three-dimensional reconstruction of single particles embedded in ice. Ultramicroscopy 40, 33–53.
28. REIMER, L. (1989). Transmission Electron Microscopy, 2nd ed. Berlin: Springer-Verlag.
29. SAYRE, D. & CHAPMAN, H. N. (1994). X-ray microscopy. Acta Cryst. A 51 (In press).
30. SAYRE, D., KIRZ, J., FEDER, R., KIM, D. M. & SPILLER, E. (1977). Transmission microscopy of unmodified biological materials: comparative radiation dosages with electrons and ultrasoft X-ray photons. Ultramicroscopy 2, 337–349.
31. SEARS, V. F. (1992). Neutron scattering lengths and cross-sections.Neutron News 3(3), 26–37.
32. SMITH, M. F. & LANGMORE, J. P. (1992). Quantitation of molecular densities in cryo-electron microscopy: determination of the radial density distribution of tobacco mosaic virus. J. Mol. Biol. 226, 763–774.
33. SPENCE, J. C. H. (1988). Experimental High-Resolution Electron Microscopy, 2nd ed., Oxford University Press.
34. STEWART, P. L., BURNETT, R. M., CYRKLAFF, M. & FULLER, S. D. (1991). Image reconstruction reveals the complex molecular organisation of Adenovirus. Cell 67, 145 - 154.
35. STEYERL, A. (1989). Ultracold neutrons: production and experiments. Physica B, 156, 528–533.
36. STEYERL, A., DREXEL, W., EBISAWA, T., GUTSMIEDLE, E., STEINHAUSER, K.-A., GAHLER, R., MAMPE, W. & AGERON, P. (1988). Neutron microscopy. Rev. Phys. Appl. 23, 171–180.
37. THON, F. (1966). Zur Defokussierungsabhangigkeit des Phasenkontrastes bei der elektronenmikroskopischen Abbildung. Z. Naturforsh. 219, 476–478.
38. TONOMURA, A. (1992). Electron-holographic interference microscopy. Adv. Physics 41, 59–103.
39. TOYOSHIMA, C., SASABE, H. & STOKES, D. L. (1993). Three-dimensional cryo-electron microscopy of the calcium ion pump in the sarcoplasmic reticulum membrane. Nature 362, 469 – 471.
40. TOYOSHIMA, C. & UNWIN, N. (1988a). Contrast transfer for frozen-hydrated specimens: determination from pairs of defocused images. Ultramicroscopy 25, 279–292.
41. TOYOSHIMA, C. & UNWIN, N. (1988). Ion channel of acetylcholine receptor reconstructed from images of postsynaptic membrane. Nature 336, 247–250.
42. ZERNIKE, F. (1955). How I discovered phase contrast. Science 121, 345–349.