Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p

Cell

Volumn 81, Issue 4, 1995, Pages 561-570

  Panzner, Steffen ^a   Dreier, Lars ^a   Hartmann, Enno ^a   Kostka, Susanne ^a   Rapoport, Tom A ^a  

^a MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN;

ARTICLE; ENDOPLASMIC RETICULUM; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN TRANSPORT; PROTEOLIPOSOME; SEQUENCE HOMOLOGY; YEAST;

MAMMALIA;

EID: 0028997459     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/0092-8674(95)90077-2     Document Type: Article

References (37)

1. Single-amino-acid substitutions within the signal sequence of yeast prepro-alpha-factor affect membrane translocation
2. A Sec63p-Bip complex from yeast is required for protein translocation in a reconstituted system
3. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation
4. A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum
5. Sec62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum
6. Assembly of yeast Sec proteins involved in translocation into the endoplasmic reticulum into a membrane-bound multisubunit complex
7. The yeast SSS1 gene is essential for secretory protein translocation and encodes a highly conserved protein of the endoplasmic reticulum
8. Sec72p contributes to the selective recognition of signal peptides by the secretory polypeptide translocation complex
9. Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum
10. Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated
11. Protein translocation across the endoplasmic reticulum. II. Isolation and characterization of the signal recognition particle receptor
12. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane
13. A mammalian homolog of Sec61 p and SecYp is associated with ribosomes and nascent polypeptides during translocation
14. Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae
15. Prepro-carboxypeptidase Y and a truncated form of pre-invertase, but not full-length invertase, can be postiranslationally translocated across microsomal membranes from Saccharomyces cerevisiae
16. In vitro protein translocation across the yeast endoplasmic reticulum: ATP-dependent posttranslational translocation of the prepro-α-factor
17. Evolutionary conservation of components of the protein translocation complex
18. Requirement for hsp70 in the mitochondrial matrix for translocation and folding of precursor molecules
19. Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus
20. Secretory protein translocation across membranes: the role of the docking protein
21. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane
22. Identification and characterization of genes and mutants for an N-terminal acetyltransferase from yeast
23. Protein translocation across the endoplasmic reticulum
24. Transport of proteins across the endoplasmic reticulum membrane
25. Secretion in yeast: reconstitution of the translocation and glycosylation of α-factor and invertase in a homologous cell-free system
26. Secretion in yeast: structural features influencing the posttranslational translocation of prepro-alpha-factor in vitro
27. Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast
28. A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia-coli heat shock protein
29. Sec61 p and BiP directly facilitate polypeptide translocation into the ER
30. Secretion in yeast: preprotein binding to a membrane receptor and ATP-dependent translocation are sequential and separable events in vitro
31. Mitochondrial Hsp70/MIM44 complex facilitates protein import
32. A protein-conducting channel in the endoplasmic reticulum
33. What drives the translocation of proteins?
34. Loss of Bip/Grp78 function blocks translocation of secretory proteins in yeast
35. Translocation of proteins across the endoplasmic reticulum. III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes
36. Secretory protein translocation in a yeast cell-free system can occur postranslationally and requires ATP hydrolysis
37. Proton motive force dependent and independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli