Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase

FEBS Letters

Volumn 371, Issue 2, 1995, Pages 94-98

  Sluis Cremer, Nicolas ^a   Dirr, Heini ^a  

^a UNIVERSITY OF THE WITWATERSRAND   (South Africa)

Author keywords

5,5 dithiobis(2 nitrobenzoic acid); Conformational stability; dithiothreitol; DTNB; DTT; Glutathione S transferase; GSH; Hydrogen peroxide; IAEDANS, N iodocetyl N (5 sulpho 1 naphthyl)ethylenediamine; Oxidative stress; pGSTP1 1, porcine glutathione S transferase class pi with two 1 type subunits; reduced glutathione; SEC HPLC; size exclusion high performance liquid chromatography; Unfolding

Indexed keywords

1,5 I AEDANS; 1,5-I-AEDANS; CYSTEINE; DITHIOTHREITOL; GLUTATHIONE; GLUTATHIONE TRANSFERASE; GUANIDINE; GUANIDINE DERIVATIVE; HYDROGEN PEROXIDE; NAPHTHALENESULFONIC ACID DERIVATIVE;

ALKYLATION; ANIMAL; ARTICLE; CHEMISTRY; ENZYME STABILITY; METABOLISM; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION; SPECTROFLUOROMETRY; STRUCTURE ACTIVITY RELATION; SWINE; THERMODYNAMICS; ANIMAL TISSUE; ENZYME ACTIVITY; ENZYME MODIFICATION; ENZYME STRUCTURE; LUNG; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN FOLDING;

ALKYLATION; ANIMAL; CYSTEINE; DITHIOTHREITOL; ENZYME STABILITY; GLUTATHIONE; GLUTATHIONE TRANSFERASE; GUANIDINE; GUANIDINES; HYDROGEN PEROXIDE; NAPHTHALENESULFONATES; OXIDATION-REDUCTION; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; SUPPORT, NON-U.S. GOV'T; SWINE; THERMODYNAMICS;

EID: 0028979030     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/0014-5793(95)00826-U     Document Type: Article

References (30)

1. Refined Crystal Structure of Porcine Class Pi Glutathione S-Transferase (pGST P1-1) at 2·1 Å Resolution
2. Porcine class pi glutathione S-transferase: anionic ligand binding and conformational analysis.
3. Native dimer stabilizes the subunit tertiary structure of porcine class pi glutathione S-transferase.