2‐μm vectors containing the Saccharomyces cerevisiae metallothionein gene as a selectable marker: Excellent stability in complex media, and high‐level expression of a recombinant protein from a CUP1‐promoter‐controlled expression cassette in cis

Yeast

Volumn 11, Issue 1, 1995, Pages 1-14

  Hottiger, Thomas ^a   Kuhla, Jochen ^a   Pohlig, Gabriele ^a   Fürst, Peter ^a   Spielmann, Albert ^a   Garn, Morten ^a   Haemmerli, Stephan ^a   Heim, Jutta ^a  

^a CIBA GEIGY LTD   (Switzerland)

Author keywords

copy number control; dominant selectable marker; heterologous proteins; hirudin; metallothionein; Yeast

Indexed keywords

COPPER; HIRUDIN; MARKER; METALLOTHIONEIN; RECOMBINANT PROTEIN; COPPER METALLOTHIONEIN;

ARTICLE; CLONING VECTOR; GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; CHROMOSOME MAP; CULTURE MEDIUM; DNA VECTOR; DRUG EFFECT; FUNGAL GENE; GENE DOSAGE; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; PLASMID; PROMOTER REGION;

HIRUDINIDA; HIRUDO MEDICINALIS; SACCHAROMYCES CEREVISIAE;

CHROMOSOME MAPPING; COPPER; CULTURE MEDIA; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GENETIC MARKERS; GENETIC VECTORS; HIRUDIN; METALLOTHIONEIN; PLASMIDS; PROMOTER REGIONS (GENETICS); SACCHAROMYCES CEREVISIAE;

EID: 0028978231     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.320110102     Document Type: Article

References (35)

1. Copper metallothionein of yeast, structure of the gene and regulation of expression
2. Yeast metallothionein and applications in biotechnology
3. Yeast metallothionein function in metal detoxification
4. Tandem gene amplification mediates copper resistance in yeast
5. Gene amplification in yeast: CUP1 copy number regulates copper resistance
6. Copper activates metallothionein transcription by altering the conformation of a specific DNA binding protein
7. The 2μ circle of Saccharomyces cerevisiae
8. A flow injection analysis system for fermentation monitoring and control
9. Regulation of the yeast metallothionein gene
10. An efficient chloramphenicol resistance marker for Saccharomyces cerevisiae and Escherichia coli
11. Function and autoregulation of yeast copperthionein
12. The transformation of brewing yeasts with a plasmid containing the gene for copper resistance
13. Physiological characterization of the yeast metallothionein promoter and consequences of overexpressing its transcriptional activator, ACE1
14. Copper‐induced binding of cellular factors to yeast metallothionein upstream activation sequences
15. The influence of GAPDH promoter variants on hirudin production, average copy number and cell growth in Saccharomyces cerevisiae
16. Primary structure and transcription of an amplified genetic locus: the CUP1 locus of yeast
17. Heterologous gene expression of the glyphosphate resistance marker and its application in yeast transformation
18. Enhancement of cloned gene product synthesis via autoselection in recombinant Saccharomyces cerevisiae
19. Influence of yeast proteases of hirudin expression in Saccharomyces cerevisiae
20. Complementation of the Saccharomyces cerevisiae srb1‐1 mutation: an autoselection system for stable plasmid maintenance
21. Propagation and expression of cloned genes in yeast: 2‐μm circle‐based vectors
22. The redundancy of ribosomal and transfer RNA genes in Saccharomyces cerevisiae
23. The sequence of the Saccharomyces cerevisiae gene PHO2 codes for a regulatory protein with unusual amino acid composition
24. Mammalian metallothionein is functional in yeast
25. ACE1 regulates expression of Saccharomyces cerevisiae metallothionein gene
26. A new system to amplify 2 μm plasmid copy number in Saccharomyces cerevisiae
27. Deoxyribonucleic acid plasmids in yeasts
28. Direct selection of Saccharomyces cerevisiae resistant to the antibiotic G418 following transformation with a DNA vector carrying the kanamycine‐resistance gene of Tn903
29. Vector YFRp1 allows transformant selection in Saccharomyces cerevisiae via resistance to formaldehyde
30. Autoregulation of the yeast metallothionein gene depends on metal binding
31. Construction of stable laboratory and industrial yeast strains expressing a foreign gene by integrative transformation using a dominant selection system