The Preparation of Catalytically Active Human Cathepsin B from Its Precursor Expressed in Escherichia coli in the Form of Inclusion Bodies

European Journal of Biochemistry

Volumn 229, Issue 2, 1995, Pages 533-539

  Kuhelj, Robert ^a   Dolinar, Marko ^a   Pungerčar, Jože ^a   Turk, Vito ^a  

^a JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

Cathepsin B; expression; processing; refolding; renaturation

Indexed keywords

CATHEPSIN B; COMPLEMENTARY DNA;

ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN PROCESSING;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATHEPSIN B; CLONING, MOLECULAR; CYSTEINE; DNA PRIMERS; ESCHERICHIA COLI; HUMAN; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PROTEIN FOLDING; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SUPPORT, NON-U.S. GOV'T;

EID: 0028958724     PISSN: 00142956     EISSN: 14321033     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1995.0533k.x     Document Type: Article

References (40)

1. Cathepsin B, cathepsin H, and cathepsin L
2. Lysosomal cysteine proteinases
3. l‐trans ‐Epoxysuccinyl‐leucylamido(4‐guanidino)butane (E‐64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L
4. A novel method for the purification of porcine phospholipase A2 expressed in E. coli
5. ab‐fragments produced in Escherichia coli
6. Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli
7. Enzymically active lysosomal proteases are associated with amyloid deposits in Alzheimer brain
8. Expression of human cathepsin B protein in Escherichia coli
9. Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs
10. Bisulfide bond formation in proteins
11. Mechanism of mineral solubilization and matrix degradation in osteoclastic bone resorption
12. Isolation, renaturation, and formation of disulfide bonds of eukaryotic proteins expressed in Escherichia coli as inclusion bodies
13. Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B
14. Co‐localization of molecules involved in antigen processing and presentation in an early endocytic compartment
15. Biosynthesis of cathepsin B in cultured normal and I‐cell fibroblasts
16. Characterization of recombinant rat cathepsin B and nonglycosylated mutants expressed in yeast
17. Development of an optimized refolding process for recombinant Ala‐Glu‐IGF‐1
18. Folding proteins
19. Conformation, structure and activation of bovine cathepsin D
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. Proteolytic processing and glycosylation of cathepsin B
22. Activation of procathepsin B in human hepatoma cells: the conversion into the mature enzyme relies on the action of cathepsin B itself
23. Maturation of human procathepsin B
24. Expression of rat and mouse cathepsin B precursors in Escherichia coli
25. The refined 2.15 A X‐ray crystal structure of human liver cathepsin B: the structural basis for its specificity
26. Identification of latent procathepsins B and L in microsomal lumen: characterization of enzymic activation and proteolytic processing in vitro
27. Amino acid sequence of human liver cathepsin B
28. Rat procathepsin B, proteolytic processing to the mature form in vitro
29. Formation of three‐dimensional structure in proteins. I. Rapid nonenzymic reactivation of reduced lysozyme
30. Intramolecular chaperones and protein folding
31. Regulation of lysosomal endo‐peptidases in malignant neoplasia
32. Activity and deletion analysis of recombinant human cathepsin L expressed in Escherichia coli
33. Use of T7 RNA polymerase to direct expression of cloned genes
34. Porcine spleen cathepsin B is an exopeptidase
35. The proregion of cathepsin L is required for proper folding, stability and ER exit
36. Active papain renatured and processed from insoluble recombinant propapain expressed in Escherichia coli
37. Cysteine proteinase activity in arthritic rat knee joint and the effect of a selective systemic inhibitor, Z‐Phe‐AlaCH2F
38. The expression in Escherichia coli of a synthetic gene coding for the precursor of papain is prevented by its own putative signal sequence
39. Expression in Escherichia coli of a chemically synthesized gene for a mini‐C analog of human pro‐insulin
40. Some characterizations of cathepsin B and α‐N‐benzoylarginine‐β‐naphtylamide hydrolase from bovine lymph nodes