Binding strength between cell adhesion proteoglycans measured by atomic force microscopy

Science

Volumn 267, Issue 5201, 1995, Pages 1173-1175

  Dammer, Ulrich ^a   Popescu, Octavian ^b,d   Wagner, Peter ^c   Anselmetti, Dario ^a   Güntherodt, Hans Joachim ^a   Misevic, Gradimir N ^b  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITY HOSPITAL BASEL   (Switzerland)

^c ETH ZURICH   (Switzerland)

^d Institute for Biological Research   (Romania)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION MOLECULE; PROTEOGLYCAN;

ANATOMY; ARTICLE; ATOMIC FORCE MICROSCOPY; BINDING AFFINITY; BINDING KINETICS; CELL ADHESION; CONTROLLED STUDY; FORCE; NONHUMAN; PRIORITY JOURNAL; SPONGE (PORIFERA);

PORIFERA;

EID: 0028949325     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.7855599     Document Type: Article

References (25)

1. C. A. Helm, W. Knoll, J. N. Israelachvili, Proc. Natl. Acad. Sci. U.S.A. 88, 8169 (1991); D. E. Leckband, J. N. Israelachvili, F.-J. Schmitt, W. Knoll, Science 255, 1419 (1992).
2. C. A. Helm, W. Knoll, J. N. Israelachvili, Proc. Natl. Acad. Sci. U.S.A. 88, 8169 (1991); D. E. Leckband, J. N. Israelachvili, F.-J. Schmitt, W. Knoll, Science 255, 1419 (1992).
3. G. Binnig, C. F. Quate, C. Gerber, Phys. Rev. Lett. 56, 930 (1986).
4. G. U. Lee, D. A. Kidwell, R. C. Colton, Langmuir 10, 354 (1994); E.-L. Florin, V. T. Moy, H. E. Gaub, Science 264, 415 (1994); U. Dammer et al., paper presented at the NATO ASI Workshop, Schluchsee, Germany, 6 to 18 March 1994 (Kluwer, Dordrecht, Netherlands, in press).
5. G. U. Lee, D. A. Kidwell, R. C. Colton, Langmuir 10, 354 (1994); E.-L. Florin, V. T. Moy, H. E. Gaub, Science 264, 415 (1994); U. Dammer et al., paper presented at the NATO ASI Workshop, Schluchsee, Germany, 6 to 18 March 1994 (Kluwer, Dordrecht, Netherlands, in press).
6. G. U. Lee, D. A. Kidwell, R. C. Colton, Langmuir 10, 354 (1994); E.-L. Florin, V. T. Moy, H. E. Gaub, Science 264, 415 (1994); U. Dammer et al., paper presented at the NATO ASI Workshop, Schluchsee, Germany, 6 to 18 March 1994 (Kluwer, Dordrecht, Netherlands, in press).
7. M. Radmacher, M. Fritz, H. G. Hansma, P. K. Hansma, Science 265, 1577 (1994).
8. J. T. Finer, R. M. Simmons, J. A. Spudich, Nature 368, 113 (1994).
9. T. Humphreys, Dev. Biol. 8, 27 (1963); C. B. Caudwell, P. Henkert, T. Humphreys, Biochemistry 12, 3051 (1973).
10. T. Humphreys, Dev. Biol. 8, 27 (1963); C. B. Caudwell, P. Henkert, T. Humphreys, Biochemistry 12, 3051 (1973).
11. G. N. Misevic, J. Finne, M. M. Burger, J. Biol. Chem. 262, 5870 (1987); G. N. Misevic and M. M. Burger, ibid. 268, 4922 (1993); D. Spillmann et al., ibid., p. 13378.
12. G. N. Misevic, J. Finne, M. M. Burger, J. Biol. Chem. 262, 5870 (1987); G. N. Misevic and M. M. Burger, ibid. 268, 4922 (1993); D. Spillmann et al., ibid., p. 13378.
13. G. N. Misevic, J. Finne, M. M. Burger, J. Biol. Chem. 262, 5870 (1987); G. N. Misevic and M. M. Burger, ibid. 268, 4922 (1993); D. Spillmann et al., ibid., p. 13378.
14. J. E. Jumblatt, V. Schlup, M. M. Burger, Biochemistry 19, 1038 (1980).
15. 2+.
16. 2+.
17. 2+.
18. Approach-and-retract cycles are sometimes called force distance curves or force plots. While the cantilever deflection was permanently monitored, the tip was slowly moved (0.1 Hz) toward the substrate until contact and was then retracted. The hysteresis is a direct measure of the adhesion force. The stiffness of the cantilevers (Park Scientific Instruments, Mountain View, CA) was verified within 30% by taking approach-and-retract cycles on top of three different Si levers that had been individually calibrated by the manufacturer.
19. For steric and statistical reasons, the approach-and-retract cycles did not always result in an adhesion peak. The frequency is a measure of interaction probabilities.
20. D. J. Rice and T. Humphreys, J. Biol. Chem. 258, 6394 (1983).
21. This length assumes that the g200 acidic glycan had the extended structure of a hyaluronate polyanion [W. T. Winter and S. Arnott, J. Mol. Biol. 117, 761 (1977)].
22. A Lennard-Jones (12-6) potential has two free parameters that can be adjusted with respect to the experimental parameters: binding energy (348 kJ/ mol) and binding length (0.154 nm). For an individual C-C bond, the gradient of the potential is the negative force and the calculated maximum is the rupture force.
23. 3).
24. We selected imaging of physiosorbed rather than covalently attached APs because (i) on a nonfunctionalized mica substrate the polysaccharide AP arms are also attached to the surface, and (ii) the smoothness of the mica facilitated imaging of the very fine AP arms. For the EM images, the mica was charged by incubation in 3-aminopropyltriethoxysilane (APTS) (Fluka) for 4 min at room temperature. Images are similar to those in S. Humphreys, T. Humphreys, J. Sano, J. Supramol. Struct. 7, 339 (1977).
25. We thank D. Hartman, Z. Markovic, and G. De Libero for helpful discussions and critical reading of this manuscript, M. Dreier and M. Hegner for useful comments, E. Paris for technical assistance, and M. Häner for the EM imaging. Supported by Swiss National Science Foundation grants 2000-037496.93 (to H.-J.G.), 3100-037733.93, and 3100-039721.93 (to G.N.M,), and Cancer League Basel grant 5354 (to G.N.M.).