Expression of thioredoxin random peptide libraries on the escherichia coli cell surfeice as functional fusions to flagellin: A system designed for exploring protein-protein interactions

Bio/Technology

Volumn 13, Issue 4, 1995, Pages 366-372

  Lu, Zhijian ^a   Murray, Kristin S ^a   Van Cleave, Victor ^a   Lavallie, Edward R ^a   Stahl, Mark L ^a   McCoy, John M ^a  

^a PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLAGELLIN; HYBRID PROTEIN; THIOREDOXIN;

AMINO ACID SEQUENCE; ARTICLE; ESCHERICHIA COLI; PEPTIDE MAPPING; PLASMID; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BINDING SITES; BLOTTING, WESTERN; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPE MAPPING; ESCHERICHIA COLI; FLAGELLIN; GENE EXPRESSION; GENETIC VECTORS; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; THIOREDOXIN;

EID: 0028947997     PISSN: 0733222X     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0495-366     Document Type: Article

References (33)

1. Smith, O. P. 1985. Filamentaous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228:1313-1317.
2. Parmley S. F. and Smith, O. P. 1988. Antibody-selectable filamentous of phage vectors: affinity purification of target genes. Gene 73:303-318.
3. Scott, I. K. and Craig, L. 1994. Random peptide libraries. Curr. Opin. Biotech-nol. 5:40-48.
4. Hammer, J., Takacs, B., and Sinigaglia, F. 1992. Identification of a motif for HLA-DR1 binding peptides using M13 display libraries. J. Exp. Med. 176:1007-1013.
5. Oldenberg, K. R., Loganathan, D., Goldstein, I. J., Schultz, P. G. and Gallop, M. A. 1992. Peptide ligands for a sugar-binding protein isolated from a random peptide library. Proc. Nat. Acad. Sci. USA 89:5393-5397.
6. Scott, J. K., Loganathan, D., Easley, R. B., Gong, X. and Goldstein, I. J. 1992. A family of concanavalin A-bindisg peptides from a hexapeptide epitope library. Proc. Nat. Acad. Sci. USA 89:5398-5402.
7. Blond-Elguindi, S., Cwirla, S. E., Dower, W. J., Lipshutz, R. J., Sprang, S. R., Sambrook, J. F. and Geming, M.-J. H. 1993. Affinity panning a library of peptides displayed on bacteriophage reveals the binding specificity of Bib. Cell 75:717-728.
8. Djojonegoro, B. M., Benedik, M. J. and Willson, R. C. 1994. Bacteriophage surface display of an immunoglobulin-bindiog domain of Staphylococcus aureus protein A. Bio/Technology 12:169-172.
9. Kam, S. K., LeMaster, D. M. and Eklund, H. 1990. Crystal structure of thioredoxin from E. coli at 1.68 angstroms resolution. J. Mol. Biol. 212:167-184.
10. La Valile, E. R., Diblasio, E. A., Kovacic, S., Grant, K. L., Sehende), P. F. and McCoy, J. M. 1993. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology 11:1187-193.
11. Scott, J. K. and Smith, G. P. 1990. Searching for peptide ligands with an epitope library. Science 249:386-390.
12. LaVallie, E. R. and Stahl, M. L. 1989. Cloning of the flagellin gene from Bacillus subtitis and complementation studies of an in vitro-derived deletion mutation. J. Bacterid. 171:3085-3094.
13. Namba, K., Yamashita, I. and Vònderviszt, F. 1989. Structure of the core and central channel of bacterial flagella. Nature 342:648-654.
14. Wilson, A. R. and Beveridge, T. J. 1993. Bacterial flagellar filaments and their component flagelline. Can. J. Microbiol. 39:451-472.
15. Kuwajima, G. 1988. Construction of a minimum-size functional flagellin of Escherichia coli. J. Bacterid. 178:3305-3309.
16. Joys, T. M. 1988. The flagellar filament protein. Can. J. Microbiol. 34:452-458.
17. Diderichsen, B. 1980. flu, a metastable gene controlling surface properties of Esckerchia coli. i. Bacteriol. 141:858-867.
18. Ofek, I. and Beachy, E. H. 1978. Mannose binding and epithelial cell adherence of Escherichia coli. Infect. Immun. 22:247-254.
19. Ponniah, S., Endres, R. O., Hasty, D. L. and Abraham, S. N. 1991. Fragmentation of E. coli type 1 fimbriae exposes cryptic I>nvmnose-binding sites. J. Bacteriol. 173:4195-4202.
20. Clore, G. M., Apella, E., Yamada, M., Matsushima, K. and Gronenbom, A. M. 1990. Three dimensional structure of interleukin 8 in solution. Biochemistry 29:1689-1696.
21. Hutchcroft, J. E., Anostario, M., Jr., Harrison, M. L., and Geahlen, R. L. 1991. Renani ration and assay of protein kinases after electrophoresis in sodium dode-cyl sulfate-polyacrylamide gels. Method in Enzymol. 200:417-423.
22. Shackelford, D. A. and Zivin, J. A. 1993. Renaturation of calcium/calmodulin-dependent protein kinase activity after electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels to membranes. Anal. Biochem. 211:131-138.
23. Charbit, A., Molla, A., Saurín, W. and Hofnung, M. 1988. Versatility of a vector for expressing foreign polypeptides at the surface of Gram-negative Bacteria. Gene 70:18-89.
24. Brown, S. 1992. Engineered iron oxide-adhesion mutants of the Escherichia coli phage λ receptor. Proc. Natl. Acad. Sci. USA 89:8651-8655.
25. Francisco, J. A., Campbell, R., Iverson, B. L. and Georgiou, G. 1993. Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Proc. Natl. Acad. Sci. USA 90:10444-10448.
26. McLafferty, M. A., Kent, R. B, Ladner, R. C. and Markland, W. 1993. M13 bacteriophage displaying disulfide-constrained microproteins. Gene 128:29-36.
27. Fedorov, O. V. and Efimov, A. V. 1990. Flagellin as an object for supramoiecular engineering. Protein Eng. 3:411-413.
28. Jessen, T., Cohen, B., Colas, P., Mendelsohn, A. M., McCoy, J. and Brent, R. 1994. Artificial peptide inhibitors of Cdk2-dependent cell proliferation. Manuscript in préparation.
29. Holmgren, A. 1985. Thioredoxin. Ann. Rev. Biochem. 54:237-271.
30. Wiirfel, M., Haberlein, I and Bollmann, H. 1993. Facile sulfitolysis of the disulfide bonds in oxidized thioredoxin and glutaredoxin. Eur. J. Biochem. 2U:609-610.
31. Brent, R. and Ptashne, M. 1981. Mechanism of action of the lex A gene product. Proc. Nat. Acad. Sci. USA 78:4204-4208.
32. Blair, D. F., Kim, D. Y. and Berg, H. C. 1991. Mutant MotB proteins in Escherichia coli Bacteriol. 173:4049-4055.
33. Norrander, I., Kempe, T. and Messing, J. 1983. Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26:101-106.