Overexpression of Ribonucleotide Reductase in Transfected Human KB Cells Increases Their Resistance to Hydroxyurea: M2 but not M1 Is Sufficient to Increase Resistance to Hydroxyurea in Transfected Cells

Cancer Research

Volumn 55, Issue 6, 1995, Pages 1328-1333

  Zhou, Bing Sen ^a   Pan, Bi Cheng ^a   Doroshow, James H ^a   Yen, Yun ^a   Hsu, Nan Yung ^b  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

^b TAICHUNG VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DNA; HYDROXYUREA; MESSENGER RNA; RIBONUCLEOTIDE REDUCTASE;

ARTICLE; CANCER RESISTANCE; CONTROLLED STUDY; DNA SYNTHESIS; DNA TRANSFECTION; ENZYME SUBUNIT; ENZYME SYNTHESIS; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; OROPHARYNX CARCINOMA; PLASMID; PRIORITY JOURNAL;

BLOTTING, SOUTHERN; DNA; DRUG RESISTANCE; HUMAN; HYDROXYUREA; KB CELLS; PLASMIDS; RIBONUCLEOTIDE REDUCTASES; RNA, MESSENGER; TRANSFECTION;

EID: 0028928261     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Cory, J. G., and Sato, A. Regulation of ribonucleotide reductase activity in mammalian cells. Mol. Cell. Biochem., 53: 257–266, 1983.
2. Thelander, L., and Berg, P. Isolation and characterization of expressible cDNA clones encoding the M1 and M2 subunits of mouse ribonucleotide reductase. Mol. Cell. Biol., 6: 3433–3442, 1986.
3. Chang, C. H., and Cheng, Y. C. Substrate specificity of human ribonucleotide reductase from Molt-4F cells. Cancer Res., 39: 5081–5086, 1979.
4. Thelander, M., Graslund, A., and Thelander, L. Subunit M2 of mammalian ribonucleotide reductase. J. Biol. Chem., 260: 2737–2741, 1985.
5. Yen, Y., Grill, S. P., Dutschman, G. E., Chang, C. N., Zhou, B. S., and Cheng, Y. C. Characterization of a hydroxyurea-resistant human KB cell line with supersensitivity to 6-thioguanine. Cancer Res., 54: 3686–3691, 1994.
6. Tagger, A. Y., and Wright, J. A. Molecular and cellular characterization of drug resistant hamster cell lines with alterations in ribonucleotide reductase. Int. J. Cancer, 42: 760–766, 1988.
7. Albert, D. A., Nodzenski, E., Yim, G., and Kowalski, J. Effect of cyclic AMP on the cell cycle regulation of ribonucleotide reductase M2 subunit messenger RNA concentrations in wild-type and mutant S49 T lymphoma cells. J. Cell. Physiol., 143: 251–256, 1990.
8. Engstrom, Y., Eriksson, S., Jildevik, I., Skog, S., Thelander, L., and Tribukait, B. Cell cycle-dependent expression of mammalian ribonucleotide reductase. Differential regulation of the two subunits. J. Biol. Chem., 260: 9114–9116, 1985.
9. Bjorklund, S., Skog, S., Tribukait, B., and Thelander, L. S-phase-specific expression of mammalian ribonucleotide reductase Rl and R2 subunit mRNAs. Biochemistry, 29: 5452–5458, 1990.
10. Ali, M. A., McWeeney, D., Milosavljevic, A., Jurka, J., and Jariwalla, R. J. Enhanced malignant transformation induced by expression of a distinct protein domain of ribonucleotide reductase large subunit from herpes simplex virus type 2. Proc. Natl. Acad. Sci. USA, 88: 8257–8261, 1991.
11. Bjorklund, S., Hjortsberg, K., Johansson, E., and Thelander, L. Structure and promoter characterization of the gene encoding the large subunit (Rl protein) of mouse ribonucleotide reductase. Proc. Natl. Acad. Sci. USA, 90: 11322–11326, 1993.
12. McClarty, G. A., Chan, A. K. M., and Wright, J. A. Hydroxyurea-induced conversion of mammalian ribonucleotide reductase to a form hypersensitive to bleomycin. Cancer Res., 46: 4516–4521, 1986.
13. Hurta, R. A., Samuel, S. K., Greenberg, A. H., and Wright, J. A. Early induction of ribonucleotide reductase gene expression by transforming growth factor β-1 in malignant H-ras transformed cell lines. J. Biol. Chem., 266: 24097–24100, 1991.
14. Hurta, R. A., and Wright, J. A. Alterations in the activity and regulation of mammalian ribonucleotide reductase by chlorambucil, a DNA damaging agent. J. Biol. Chem., 267: 7066–7071, 1992.
15. Choy, B. K., McClarty, G. A., and Wright, J. A. Transient elevation of ribonucleotide reductase activity, M2 mRNA and M2 protein in BALA/c 3T3 fibroblasts in the presence of 12-O-tetradecanoylphorbol-13-acetate. Biochem. Biophys. Res. Com-mun., 162: 1417–1424, 1989.
16. Mann, G. J., Musgrove, E. A., Fox, R. M., and Thelander, L. Ribonucleotide reductase Ml subunit in cellular proliferation, quiescence, and differentiation. Cancer Res., 48: 5151–5156, 1988.
17. Srinivasan, P. R., Tonin, P. N., Wensing, E. J., and Lewis, W. H. The gene for ornithine decarboxylase is co-amplified in hydroxyurea-resistant hamster cells. J. Biol. Chem., 262: 12871–12878, 1987.
18. Hurta, R. A. R., and Wright, J. A. Mammalian drug resistant mutants with multiple gene amplification: genes encoding the Ml component of ribonucleotide reductase, the M2 component of ribonucleotide reductase, ornithine decarboxylase, p5–8, the H-subunit of ferritin and the L-subunit of ferritin. Biochem. Biophys. Acta, 1087: 165–172, 1990.
19. Luo, J. A., Smith, C. C., Kulka, M., and Aurelian, L. A truncated protein kinase domain of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (ICP10) expressed in Escherichia coll J. Biol. Chem., 266: 20976–20983, 1991.
20. Jones, C., Zhu, F., and Dhanwada, K. R. Analysis of a herpes simplex virus 2 fragment from the open reading frame of the large subunit of ribonucleotide reductase with transcriptional regulatory activity. DNA Cell Biol., 12: 127–137, 1993.
21. Desai, P., Ramakrishnan, R., Lin, Z. W., Osak, B., Glorioso, J. C., and Levine, M. The RR1 gene of herpes simplex virus type 1 is uniquely transactivated by ICPO during infection. J. Virol., 67: 6125–6135, 1993.
22. Lankinen, H., Everett, R., Cross, A., Conner, J., and Marsden, H. S. Epitope mapping identifies an exposed loop between the unique amino- and conserved carboxy-domains of the large subunit of herpes simplex virus type 1 ribonucleotide reductase. J. Gen. Virol., 74: 1871–1877, 1993.
23. Salem, J. S., Scott, C. P., Li, L. H., Cooperman, B. S., and Rubin, H. High level expression of the large subunit of mouse ribonucleotide reductase in a baculovirus system. FEBS, 323: 93–95, 1993.
24. Howell, M. L., Sanders-Loehr, J., Loehr, T. M., Roseman, N. A., Mathews, C. K., and Slabaugh, M. B. Cloning of the Vaccinia virus ribonucleotide reductase small subunit gene. J. Biol. Chem., 267: 1705–1711, 1992.
25. Albert, D. A., and Rozengurt, E. Synergistic and coordinate subunits in Swiss 3T3 cells: effect of multiple signal-transduction pathways. Proc. Natl. Acad. Sci. USA, 89: 1597–1601, 1992.
26. Pavloff, N., Rivard, D., Masson, S., Shen, S. H. H., and Mes-Masson, A. M. Sequence analysis of the large and small subunits of human RR. DNA Seq., 2: 227–234,1992.
27. Wehland, J., Schroder, H. C., and Weber, K. Amino acid sequence requirements in the epitope recognized by the tubulin-specific rat monoclonal antibody YL 1/2. EMBO J., 3: 1295–1300, 1984.
28. Steeper, J. P., and Stuart, C. D. A rapid assay for CDP reductase activity in mammalian cell extracts. Anal. Biochem., 34: 123–130, 1970.
29. Gunning, P., Leavitt, J., Muscat, G., Ng, S.-Y., and Kedes, L. A human 0-actin expression vector system directs high-level accumulation of antisense transcripts. Proc. Natl. Acad. Sci. USA, 84: 4831–4835, 1987.
30. Finlay, G. J., Baguley, B. C., and Wilson, W. R. A semiautomated microculture method for investigation exponentially growing carcinoma cells. Anal. Biochem., 139: 272–277, 1984.
31. Thelander, M., and Thelander, L. Molecular cloning and expression of the functional gene encoding the M2 subunit of mouse ribonucleotide reductase: a new dominant marker gene. EMBO J., 8: 2475–2479, 1989.
32. McClarty, G. A., Chan, A. K., Choy, B. K., Thelander, L., and Wright, J. A. Molecular mechanisms responsible for the drug-induced posttranscriptional modulation of ribonucleotide reductase levels in a hydroxyurea-resistant mouse L cell line. Biochemistry, 27: 7524–7531, 1988.
33. McClarty, G. A., Chan, A. K., Engstrom, Y., Wright, J. A., and Thelander, L. Elevated expression of Ml, M2 components and drug-induced posttranscriptional modulation of ribonucleotide reductase in a hydroxyurea-resistant mouse cell line. Biochemistry, 26: 8004–8011, 1987.