Detection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization

Archives of Microbiology

Volumn 163, Issue 4, 1995, Pages 235-241

  Hönerlage, Wolfgang ^a   Hahn, Dittmar ^a   Zeyer, Josef ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Bacillus megaterium; Extracellular neutral protease; In situ detection of mRNA; In vitro transcripts; nprM; Whole cell hybridization

Indexed keywords

MESSENGER RNA; PROTEINASE;

ARTICLE; BACILLUS MEGATERIUM; CELL HYBRIDIZATION; GENETIC TRANSCRIPTION; GROWTH CURVE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; SOIL;

EID: 0028926922     PISSN: 03028933     EISSN: 1432072X     Source Type: Journal    
DOI: 10.1007/BF00393374     Document Type: Article

References (44)

1. Combination of 16S rRNA-targeted probes with flow cytometry for analyzing mixed microbial populations
2. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology
3. Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms
4. Method for blot-hybridization analysis of mRNA molecules from Bacillus subtilis
5. Characterization of subsurface bacteria associated with two shallow aquifers in Oklahoma
6. In situ hybridization of nodulin mRNAs in root nodules using non-radioactive probes
7. Genomic sequencing
8. Whole mount in situ detection of low abundance transcripts of the myogenic factor qmfl and myosin heavy chain protein in quail embryos
9. Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis
10. Single, chemically defined sporulation medium for Bacillus subtilis: growth, sporulation, and extracellular protease production
11. Detection of micro-organisms in soil after in situ hybridization with rRNA-targeted, fluorescently labelled oligonucleotides
12. Whole-cell hybridization of Frankia strains with fluorescence- or digoxigenin-labeled, 16S rRNA-targeted oligonucleotide probes
13. Detection of mRNA in Streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes
14. Oligonucleotide probes for the detection and identification of bacteria
15. spoO genes, the phosphorelay, and the initiation of sporulation
16. Non-radioactive labeling of RNA transcripts in vitro with the hapten digoxigenin (DIG); hybridization and ELISA-based detection
17. Preparation of RNA from cotton leaves and pollen
18. Molecular cloning and nucleotide sequence of the gene encoding a calcium-dependent exoproteinase from Bacillus megaterium ATCC 14581
19. Simultaneous and rapid isolation of bacterial and eukaryotic DNA and RNA: a new approach for isolating DNA
20. Phylogenetic oligonucleotide probes for the major subclasses of proteobacteria: problems and solutions
21. Development of an oligonucleotide probe targeting 16S rRNA and its application for detection and quantification of the ruminal bacterium Synergistes jonesii in a mixed-population chemostat
22. Transcriptional and post-transcriptional control of the Bacillus subtilis succinate dehydrogenase operon
23. Etude de la mégatériopeptidase, protéase exocellulaire de Bacillus megaterium. III. Biosynthèse et rôle physiologique
24. Sporulation, germination and out-growth
25. Extracellular enzyme synthesis in the genus Bacillus
26. Systematics and ecology of Bacillus
27. Survival strategies of bacteria in the natural environment
28. Sequential induction of nodulin gene expression in the developing pea nodule
29. Procaryotes and their habitats
30. Nucleic acid probes for the identification and in situ detection of Pseudomonads
31. Approved list of bacterial names
32. Regulatory proteins that control late-growth development
33. Dominating role of an unusual magnetotactic bacterium in the microaerobic zone of a fresh water sediment
34. Naturally occurring fluorescent pseudomonads involved in suppression of black root rot of tobacco
35. Development of genetic engineering in Bacillus megaterium
36. Prime time for Bacillus megaterium
37. Probing activated sludge with oligonucleotides specific for proteobacteria: inadequacy of culture-dependent methods for describing microbial community structure
38. Distribution and identification of proteolytic Bacillus spp. in paddy field soil under rice cultivation
39. Source of soil protease in paddy fields
40. Nodulin gene expression and ENOD2 localization in effective, nitrogen-fixing and ineffective, bacteriafree nodules of alfalfa
41. Identification of single bacterial cells using digoxigenin-labelled rRNA-targeted oligonucleotides