Immunodiagnostic studies on Onchocerca volvulus and mansonella perstans infections using a recombinant 33 kDa O. volvulus protein (Ov33)

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 89, Issue 1, 1995, Pages 51-54

  Tawill, S A ^a   Buttner D W ^a,b   Lucius, R ^c   Gallin, M ^a   Erttmann, K D ^a  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

^b Gesellschaft für Technische Zusammenarbeit mbH   (Uganda)

^c UNIVERSITY OF HOHENHEIM   (Germany)

Author keywords

Filariasis; Mansonella perstans; Onchocerca volvulus; Onchocerciasis; Serodiagnosis

Indexed keywords

DETERGENT; GLUTATHIONE TRANSFERASE; PROTEIN;

ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; DISEASE CARRIER; ENZYME LINKED IMMUNOSORBENT ASSAY; EXTRACT; FILARIASIS; FILARIOIDEA; HUMAN; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; ONCHOCERCA VOLVULUS; ONCHOCERCIASIS; SERODIAGNOSIS; SKIN BIOPSY; UGANDA;

ANIMALS; ANTIGENS, HELMINTH; BLOTTING, WESTERN; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HELMINTH PROTEINS; HUMANS; MANSONELLA; MANSONELLIASIS; ONCHOCERCA VOLVULUS; ONCHOCERCIASIS; RECOMBINANT PROTEINS; SKIN DISEASES, PARASITIC;

FILARIOIDEA; MANSONELLA PERSTANS; ONCHOCERCA VOLVULUS;

EID: 0028924205     PISSN: 00359203     EISSN: 18783503     Source Type: Journal    
DOI: 10.1016/0035-9203(95)90656-8     Document Type: Article

References (15)

1. Albiez, E. J., Buttner, D. W. & Duke, B. O. L. (1988). Diagnosis and extirpation of nodules in human onchocerciasis. Tropical Medicine and Parasitology, 39, 331-346.
2. Bradley, J. E., Helm, R., Lahaise, M. & Maizels, R. M. (1991). cDNA clones of Onchocerca volvulus low molecular weight antigens provide immunologically specific diagnostic probes. Molecular and Biochemical Parasitology, 46, 219-228.
3. Burnette, W. N. (1981). ‘Western blotting’: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacryla- mide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry, 112, 195-203.
4. Chandrashekar, R., Masood, K., Alvarez, R. M., Ogunrinade, A. F., Lujan, R., Richards, F. O. & Weil, G. J. (1991). Molecular cloning and characterization of recombinant parasite antigens for immunodiagnosis of onchocerciasis. Journal of Clinical Investigation, 88, 1460-1466.
5. Fischer, P., Kipp, W., Bamuhiga, J., Binta-Kahwa, J., Kiefer, A. & Buttner, D. W. (1993). Parasitological and clinical characterization of Simulium neaoei-transmitted onchocerciasis in western Uganda. Tropical Medicine and Parasitology, 44, 311-321.
6. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
7. Lightowlers, M. W. & Mitchell, G. F. (1989). Assessment of the prevalence and titer of antibodies to a candidate schistosomiasis vaccine molecule, Sj 26, in several human serum banks. Acta Tropica, 46, 229-238.
8. Lobos, E., Weiss, N., Karam, M., Taylor, H. R., Ottesen, E. A. & Nutman, T. B. (1991). An immunogenic Onchocerca volvulus antigen: A specific and early marker of infection. Science, 251, 1603-1605.
9. Lucius, R., Schulz-Key, H., Buttner, D. W., Kern, A., Kalt- mann, B., Prod-hon, J., Seeber, F., Walter, R. D., Saxena, K. C. & Diesfeld, H. J. (1988a). Characterization of an immunodominant Onchocerca volvulus antigen with patient sera and a monoclonal antibody. Journal of Experimental Medicine, 167, 1505-1510.
10. Lucius, R., Erondu, N., Kern, A. & Donelson, J. E. (1988b). Molecular cloning of an immunodominant antigen of Onchocerca volvulus. Journal of Experimental Medicine, 168, 1199-1204.
11. Lucius, R., Kern, A., Seeber, F., Pogonka, T., Willenbucher, J., Taylor, H. R., Pinder, M., Ghalib, H. W., Schulz-Key, H. & Soboslay, P. (1992). Specific and sensitive IgG4 immunodiagnosis of onchocerciasis with a recombinant 33 kD Onchocerca volvulus protein (Ov33). Tropical Medicine and Parasitology, 43, 139-145.
12. Lustigman, S., Brotman, B, Huima, T. & Prince, A. M. (1991). Characterisation of an Onchocerca volvulus cDNA clone encoding a genus specific antigen present in infective larvae and adult worms. Molecular and Biochemical Parasitology, 45, 65-76.
13. Nutman, T. B., Steel, C., Ward, D. J., Zea-Flores, G. & Ottesen, E. A. (1991). Immunity to onchocerciasis: Recognition of larval antigens by humans putatively immune to Onchocerca volvulus infection. Journal of Infectious Diseases, 163, 1128-1133.
14. Schulz-Key, H., Albiez, E. J. & Buttner, D. W. (1977). Isolation of living adult Onchocerca volvulus from nodules. Tropen- medizin undParasitologie, 28, 428-430.
15. Taylor, H. R., Munoz, B., Keyvan-Larijani, E. & Greene, B. M. (1989). Reliability of detection of microfilariae in skin snips in the diagnosis of onchocerciasis. American Journal of Tropical Medicine and Hygiene, 41, 467-471.