Comparison of HLA‐DRB1 typing by DNA‐RFLP, PCR‐SSO and PCR‐SSP methods and their application in providing matched unrelated donors for bone marrow transplantation

Tissue Antigens

Volumn 45, Issue 2, 1995, Pages 103-110

  Jordan, F ^a   McWhinnie, A J ^a   Turner, S ^a   Gavira, N ^a   Calvert, A A ^a   Cleaver, S A ^a   Holman, R H ^a   Goldman, J M ^a   Madrigal, J A ^a  

^a ROYAL FREE HOSPITAL   (United Kingdom)

Author keywords

BMT; HLA DR; PCR SSO; PCR SSP; RFLP

Indexed keywords

HLA DQ ANTIGEN; HLA DR ANTIGEN;

ARTICLE; BLOOD ANALYSIS; BONE MARROW TRANSPLANTATION; DATA ANALYSIS; DNA DETERMINATION; DONOR; HLA MATCHING; HLA TYPING; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; NORMAL HUMAN; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RECIPIENT; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

BASE SEQUENCE; BONE MARROW TRANSPLANTATION; COMPARATIVE STUDY; DNA PRIMERS; EVALUATION STUDIES; GENES, MHC CLASS II; HISTOCOMPATIBILITY TESTING; HLA-DR ANTIGENS; HUMAN; MASS SCREENING; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; ORGAN PROCUREMENT; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; TISSUE DONORS;

EID: 0028918795     PISSN: 00012815     EISSN: 13990039     Source Type: Journal    
DOI: 10.1111/j.1399-0039.1995.tb02425.x     Document Type: Article

References (23)

1. Results of bone marrow transplants from, unrelated donors
2. Effect of HLA compatibility on engraftment of bone marrow transplants in patients with leukaemia and lymphoma
3. Bone marrow transplantation for chronic myeloid leukaemia: the use of histocompatible unrelated volunteer donors
4. Analysis of 462 transplantations from unrelated donors facilitated by National Marrow Donor Programme
5. Selection of unrelated donor for marrow transplantation facilitated by the molecular characterization of a novel HLA allele
6. Nomenclature for HLA‐A and HLA‐B alleles detected by ID‐IEF gel electrophoresis
7. Determination of the HLA‐A19 groups of alleles by PCR amplification with sequence‐specific primers (PCR‐SSP)
8. Defining the common subtypes of HLA A9, A10, A28 and A19 by use of ARMS/PCR
9. Isoelectric focusing of HLA‐A can be defined by oligonucleotide typing
10. Polymerase‐chain‐reaction‐based analysis of polymorphism in the HLA‐B gene
11. DNA‐RFLP typing system that positively identifies serologically well‐defined and ill‐defined HLA‐DR and DQ alleles, including DRw10
12. Non‐radioactive oligotyping for HLA‐DR1‐DRw10 using polymerase chain reaction, digoxigenin‐labelled oligonucleotides and chemiluminescence detection
13. Genetic analysis of amplified DNA with immobilized sequence specific oligonucleotide probes
14. HLA DR typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor‐recipient matching in cadaveric transplantation
15. HLA‐DQB1 and DQA1 typing by PCR amplification with sequence‐specific primers (PCR‐SSP) in 2 hours
16. A comparative study of HLA‐DRB1 typing by standard serology and hybridization of non‐radioactive sequence‐specific oligonucleotide probes to PCR‐amplified DNA
17. HLA‐DRB and DQB typing by a combination of serology, restriction fragment length polymorphism analysis and oligonucleotide probing
18. Combined use of RFLP and PCR‐ASO typing for HLA‐DR‐Dw and DQw typing
19. A simple salting‐out procedure for extracting DNA from human nucleated cells
20. Nomenclature for factors of the HLA system
21. Detection of specific sequence among DNA fragments separated by gel electrophoresis
22. Five new Taq I DRB1 polymorphisms