Trypanosoma cruzi: Both chemically induced and triatomine-derived metacyclic trypomastigotes cause the same immunological disturbances in the infected mammalian host

Experimental Parasitology

Volumn 80, Issue 2, 1995, Pages 194-204

  Lopes, Marcela F ^a   Cunha, José Marcos T ^a   Bezerra, Fabiana L ^a   Gonzalez, Marcelo S ^b   Gomes, José Eugenio L ^b   Lapa e Silva, José Roberto ^a   Garcia, Elói S ^b   Reis, George A Dos ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Experimental Chagas' disease; fetal calf serum (FCS); Forward light scattering (FSC); Mean fluorescence intensity (MFI); Metacyclic trypomastigotes; Monoclonal antibody (mAb); triatomine artificial urine proline (TAU P); Tritiated thymidine ( 3H TdR); Trypanosoma cruzi

Indexed keywords

EID: 0028913239     PISSN: 00144894     EISSN: None     Source Type: Journal    
DOI: 10.1006/expr.1995.1024     Document Type: Article

References (25)

1. BHATTACHARYA, A., DORF, M. E., and SPRINGER, T. A. 1981. A shared alloantigenic determinant on la antigens encoded by the I-A and 1-E subregions: Evidence for I region gene duplication. Journal of Immunology 127, 2488-2495.
2. CONTRERAS, V. T., SALLES, J. M., THOMAS, N., MOREL, C. M., and GOLDENBERG, S. 1985. In vitro differentiation of Trypanosoma cruzi under chemi-cally defined conditions. Molecular and Biochemi-cal Parasitology 16, 315-327.
3. CHIARI, E., and CAMARGO, E. P. 1984. Culturing and cloning of Trypanosoma cruzi. In “Genes and Antigens of Parasites: A Laboratory Manual” (C. M. Morel, Ed.), pp. 23-28. Fundaçâo Oswaldo Cruz, World Health Organization, Rio de Janeiro.
4. D’AVILA REIS, D., JONES, E. M., TOSTES, S., LOPES, E. R., GAZZINELLI, G., COLLEY, D., and MCCUR-LEY, T. L. 1993. Characterization of inflammatory infiltrates in chronic chagasic myocardial lesions: Presence of tumor necrosis factor-a + cells and dominance of granzyme A +, CD8+ lymphocytes. American Journal of Tropical Medicine and Hygiene 48, 637-644.
5. DIALYNAS, D. P., WILDE, D. B., MARRACK, P., PIERRES, A., WALL, K. A., HAVRAN, W., OTTEN, G., LOKEN, M. R., PIERRES, M., KAPPLER, J., and FITCH, F. W. 1983. Characterization of the murine antigenic determinant L3T4a, recognized by monoclonal antibody GK 1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen reactivity. Immunological Reviews 74, 29-55.
6. GARCIA, E. S., AZAMBUJA, P., and CONTRERAS, V. T. 1984. Large-scale rearing of Rhodnius pro-lixus and production of metacyclic trypomastigotes of Trypanosoma cruzi. In “Genes and Antigens of Parasites: A Laboratory Manual” (C. M. Morel, Ed.), pp. 44-47. Fundaçâo Oswaldo Cruz, World Health Organization, Rio de Janeiro.
7. GARCIA, E. S., SUBRAHMANYAM, B., MULLER, T., ANDREMBOLD, H. 1989. Absorption, storage, organ distribution and excretion of dietary 22, 23-3H2-dihydroazadirachtin A in the blood feeding bug, Rhodnius prolixus. Journal of Insect Physiology 35, 742-749.
8. GAZZINELLI, G., and BRENER, Z. 1991. Immunological aspects of the morbidity of human Chagas’ disease. Research in Immunology 142, 167-169.
9. HAREL-BELLAN, A., JOSKOWICZ, M., FRADELIZI, D., and EISEN, H. 1985. T lymphocyte function during experimental Chagas disease: Production of and re-sponse to interleukin 2. European Journal of Immu-nology 15, 438-442.
10. JULIUS, M. H., SIMPSON, E., and HERZENBERG, L. H. 1973. A rapid method for the isolation of fune-tional thymus derived murine lymphocytes. Euro-pean Journal of immunology 3, 645-649.
11. LEDBETTER, J. A., and HERZENBERG, L. 1979. Xe-nogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunological Reviews 47, 63.
12. LEO, O., FOO, M., SACHS, D. H., SAMELSON, L. E., and BLUESTONE, J. A. 1987. Identification of a monoclonal antibody specific for a murine T3 poly-peptide. Proceedings of the National Academy of Sciences USA 84, 1374-1377.
13. LOPES, M. F., and DOSREIS, G. A. 1994. Trypanosoma crazi-induced immunosuppression: Blockade of costimulatory T cell responses in infected hosts due to defective TcR;CD3 functioning. Infection and Immunity 62, 1484-1488.
14. MICELI, M. C., and PARNÉS, J. R. 1993. Role of CD4 and CD8 in T cell activation and differentiation. Ad-vances in Immunology 53, 59-122.
15. MINOPRIO, P., EISEN, H., FORNI, L., D’IMPERIO LIMA, M. R., JOSKOWICZ, M., and COUTINHO, A. 1986. Polyclonal lymphocyte responses to murine Trypanosoma cruzi infection. I. Quantitation of both T-and B-cell responses. Scandinavian Journal of Immunology 24, 661-668.
16. RIBEIRO DOS SANTOS, R., ROSSI, M. A., LAUS, J. L., SILVA, J. S., SAVINO, W., and MENGEL, J. 1992. Anti-CD4 abrogates rejection and reestablishes long term tolerance to syngeneic newborn hearts grafted in mice chronically infected with Trypanosoma cruzi. Journal of Experimental Medicine 175, 39-49.
17. ROTTENBERG, M., CARDONI, R. L., ANDERSSON, R., SEGURA, E. L., and ÖRN, A. 1988. Role of T helper/inducer cells as well as Natural Killer cells in resistance to Trypanosoma cruzi infection. Scandi-navian Journal of Immunology 28, 573-582.
18. Russo, M., STAROBINAS, N., MINOPRIO, P., COUTINHO, A., and HONTEBEYRIE-JOSKOWICZ, M. 1988. Parasitic load increases and myocardial in-flammation decreases in Trypanosoma cruzi-infected mice after inactivation of helper T cell activities. Annales d'institut Pasteur/Immunology 139, 225-236.
19. TARLETON, R. L. 1988. Trypanosoma wuzí-induced suppression of IL2 production. II. Evidence for a role for suppressor cells. Journal of Immunology 140, 2769-2773.
20. TARLETON, R. L. 1990. Depletion of CD8+ T cells increases susceptibility and reverses vaccine-induced immunity in mice infected with Trypano-soma cruzi. Journal of Immunology 144, 717-724.
21. TARLETON, R. L., KOHLER, B. H., LATOUR, A., and POSTAN, M. 1992. Susceptibility of beta 2-micro-globulin-deficient mice to Trypanosoma cruzi infec-tion. Nature (London) 356, 338-341.
22. TARLETON, R. L., SUN, J., POSTAN, M., and ZHANG, L. 1993. T cell subsets and their roles in pathogenesis in murine Chagas’ disease. Memorias do Instituto Oswaldo Cruz 88(Suppl.), 48-49.
23. UNKELESS, J. C. 1979. Characterization of a monoclo-nal antibody directed against mouse macrophage and lymphocyte Fc receptors. Journal of Experi-mental Medicine 150, 580-596.
24. VILLALTA, F., and KIERSZENBAUM, F. 1987. Insect-borne and culture-derived metacyclic Trypanosoma cruzi: Differences in infectivity and virulence. American Journal of Tropical Medicine and Hygiene 36, 529-532.
25. WHO. 1991. “Control of Chagas Disease.” WHO Technical Report 811, pp. 27-30. Geneva, Switzer-land.