Denaturing and non-denaturing gel electrophoresis as methods for the detection of junctional diversity in rearranged T cell receptor sequences

Journal of Immunological Methods

Volumn 181, Issue 1, 1995, Pages 101-114

  Offermans, Myriam T C ^a   Sonneveld, Renate D ^a   Bakker, Egbert ^b   Deutz Terlouw, Piëtte P ^b   de Geus, Bernard ^a   Rozing, Jan ^a  

^a TNO   (Netherlands)

^b LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Heteroduplex DNA molecule; Junctional diversity; Nucleic acid gel electrophoresis; T cell heterogeneity; T cell receptor

Indexed keywords

HETERODUPLEX; T LYMPHOCYTE RECEPTOR;

ANIMAL CELL; ARTICLE; DNA SEQUENCE; GEL ELECTROPHORESIS; GENE REARRANGEMENT; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMAL; BASE SEQUENCE; COMPARATIVE STUDY; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE REARRANGEMENT, GAMMA-CHAIN T-CELL ANTIGEN RECEPTOR; INTESTINAL MUCOSA; MALE; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; POLYMORPHISM (GENETICS); RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA; RNA; SENSITIVITY AND SPECIFICITY; T-LYMPHOCYTES;

EID: 0028907142     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(94)00334-S     Document Type: Article

References (30)

1. Antigen receptors on T and B lymphocytes: parallels in organization and function
2. T cell antigen receptor genes and T cell recognition
3. Localization and phenotype of CD3 associated γδ receptor expressing intraepithelial lymphocytes
4. Two-dimensional electrophoretic separation of restriction enzyme fragments of DNA
5. DNA fragments differing by single base-pair substitutions are separated in denaturing gradient gels: Correspondence with melting theory
6. Mutation Detection by denaturing gradient gel electrophoresis (DGGE)
7. Single-strand conformation polymorphism for detection of mutations and base substitutions in Phenylketonuria
8. Computational simulation of DNA melting and its application to denaturing gradient gel electrophoresis
9. Sequence-determined DNA separations
10. Detection of p53 gene mutations in human brain tumors by single strand conformation polymorphism analysis of polymerase chain reaction products
11. Inactivation of the retinoblastoma gene in a human lung carcinoma cell line detected by single-strand conformation polymorphism analysis of the polymerase chain reaction product of cDNA
12. Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis
13. Modification of the melting properties of duplex DNA by attachment of a GC-rich DNA sequence as determined by denaturing gradient gel electrophoresis
14. Detection and localization of single base changes by denaturing gradient gel electrophoresis
15. Detection of single base changes in DNA: ribonuclease cleavage and denaturing gradient gel electrophoresis
16. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms
17. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction
18. Restricted V gene usage in T-cell lymphomas as detected by anti-T-cell receptor variable region reagents
19. Inherited polymorphism of the human T-cell antigen receptor detected by a monoclonal antibody
20. Detection of mutations in insulin-receptor gene in NIDDM patients by analysis of single-stranded conformation polymorphisms
21. Plasmid vectors
22. DNA sequencing with chain-terminating inhibitors
23. Attachment of a 40 base-pair G + C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes
24. Diversity of γδ T-cell receptors on murine intestinal intraepithelial lymphocytes
25. Somatic generation of antibody diversity
26. Improved procedure for the isolation of functionally active lymphoid cells from the murine intestine
27. Detecting single base substitutions as heteroduplex polymorphisms
28. Heteroduplex analysis can increase the informativeness of PCR-amplified VNTR markers: Application using a marker tightly linked to the COL2A1 gene
29. Structure, organization and polymorphism of murine and human T-cell receptor α and β chain gene families
30. + γδ T cells in systemic sclerosis patients