SCOPUS 정보 검색 플랫폼

Volumn 5, Issue 22, 1995, Pages 2661-2664

Phospho-serine and phospho-threonine building blocks for the synthesis of phosphorylated peptides by the Fmoc solid phase strategy

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOSERINE; PHOSPHOTHREONINE;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS;

EID: 0028886074 PISSN: 0960894X EISSN: None Source Type: Journal
DOI: 10.1016/0960-894X(95)00475-9 Document Type: Article

Times cited : (40)

References (16)

1
- 84987487370
- A Simple and Effective Chemical Phosphorylation Procedure for Biomolecules
- (1987) Helvetica Chimica Acta , vol.70 , pp. 175
- Bannwarth¹ Trzeciak²

3
- 0007412966
- Alternative Strategies for the Fmoc Solid-Phase Synthesis ofO4-Phospho-L-tyrosine-Containing Peptides
- (1991) Helvetica Chimica Acta , vol.74 , pp. 1314
- Kitas¹ Knorr² Trzeciak³ Bannwarth⁴

4
- 84987491046
- Synthesis of Multi-O4-phospho-L-tyrosine-Containing Peptides
- (1992) Helvetica Chimica Acta , vol.75 , pp. 707
- Kitas¹ Bannwarth²

6
- 85046043609
- Synthesis and Function of an O-Phosphorylated Peptide Corresponding to the Cell Adhesion Sequence of Bone Sialoprotein (BSP).
- (1993) Acta Chemica Scandinavica , vol.35 , pp. 565
- Larsson¹ Luening² Heinegard³

7
- 0000634675
- An Efficient Procedure for Solid-Phase Synthesis of Phosphopeptides by the Fmoc Strategy.
- (1994) Chemistry Letters , vol.35 , pp. 1099
- Wakamiya¹ Saruta² Yasuoka³ Kusunoto⁴

12
- 84916540278
- 10 Yields of crude peptides were 〉 80%, with respect to the initial loading of the support.

13
- 84916540277
- 6 starting from HMPB-tentagel resin esterified with Fmoc-Glu(tBu). Peptides 9 and 10 were obtained after cleavage with reagent K (2h). Yields of crude peptides 9 and 10 were 77% and 82%, respectively, based on the initial substitution of the resin. Cleavage with 80% acetic acid at 80 degrees for 1h afforded crude peptide 11 in 60 % yield.

14
- 84916540276
- The peptides were characterized by analytical HPLC (Merck RP-18, 5μm, 4 × 125 mm, Lichrospher-100Å) using an acetonitril gradient (0–80% acetonitril in 30 min, flow: 1ml/min) and UV detection at 230 nm.

15
- 84916540275
- Poros HPLC was carried out on a Poros II R/H colum (100× 4.6 mm) running a gradient from 10% B to 100% B in 10 min (A: 0.1 % TFA, B: 80% acetonitril / 20% water; flow rate: 4ml/min; UV detection: 230 nm). The partially protected peptide 11 was obtained in 16% yield, with respect to the crude material.

16
- 84916540274
- 2: Bis(benzyloxy)(diisopropylamino) phosphine; RP18-HPLC: Reversed phase (C18) high performance liquid chromatography; TBDMS: t-Butyl-dimethylsilyl; tBu: t-butyl; TMBHA: p-(R,S-α-1-, 9H-Fluoren-9-yl)methoxyformamido 2,4-dimethoxybenzyl)) phenoxyacetic acid; TPTU: 2-(2-Oxo-1(2H)-pyridyl) 1,1,3,3-tetramethyluronium tetrafluoroborate; Trt: trityl.

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.