Functional analysis reports. Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction

Yeast

Volumn 11, Issue 13, 1995, Pages 1275-1280

  Amberg, David C ^a   Botstein, David ^a   Beasley, Ellen M ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

fusion PCR; gene disruption; Saccharomyces cerevisiae

Indexed keywords

ALLELE; ARTICLE; CONTROLLED STUDY; GENE DISRUPTION; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST;

ALLELES; BASE SEQUENCE; GENES, FUNGAL; GENETIC MARKERS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

SACCHAROMYCES CEREVISIAE;

EID: 0028884177     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.320111307     Document Type: Article

References (10)

1. Defining protein interactions with yeast actin in vivo
2. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
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4. A rapid method for the construction of synthetic genes using the polymerase chain reaction
5. Guide to yeast genetics and molecular biology
6. Site‐directed mutagenesis by overlap extension using the polymerase chain reaction
7. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers
8. New heterologous modules for classical or PCR‐based gene disruption in Saccharomyces cerevisiae