The relative importance of premortem acidosis and postmortem interval for human brain gene expression studies: selective mRNA vulnerability and comparison with their encoded proteins

Neuroscience Letters

Volumn 200, Issue 3, 1995, Pages 151-154

  Harrison, P J ^a   Heath, P R ^b   Eastwood, S L ^a   Burnet, P W J ^a   McDonald, B ^a   Pearson, R C A ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Agonal state; Autopsy; Immunocytochemistry; In situ hybridization; Messenger RNA; pH; Receptor autoradiography

Indexed keywords

MESSENGER RNA;

ACIDOSIS; ADULT; ARTICLE; AUTOPSY; CONTROLLED STUDY; FEMALE; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; NORMAL HUMAN; PH; PRIORITY JOURNAL;

ACIDOSIS; ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; AUTORADIOGRAPHY; BRAIN CHEMISTRY; COMPARATIVE STUDY; FEMALE; GENE EXPRESSION; HUMAN; HYDROGEN-ION CONCENTRATION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; MIDDLE AGE; NERVE TISSUE PROTEINS; POLY A; POSTMORTEM CHANGES; RNA, MESSENGER; SUPPORT, NON-U.S. GOV'T; TIME FACTORS;

EID: 0028845078     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/0304-3940(95)12102-A     Document Type: Article

References (22)

1. How to run a brain bank: potentials and pitfalls in the use of human post-mortem brain material in research
2. Pre- and post-mortem influences on brain RNA
3. Effect of pre- and postmortem variables on specific mRNA levels in human brain
4. A single step method for the isolation of RNA using the acid-phenol-chloroform method
5. A comparison of methodologies for the study of functional transmitter neurochemistry in human brain
6. Decreased expression of mRNAs encoding non-NMDA glutamate receptors GluR1 and GluR2 in medial temporal lobe neurons in schizophrenia
7. Altered synaptophysin expression as a marker of synaptic pathology in schizophrenia
8. The patients dying after long terminal phase have acidotic brains: implications for biochemical measurements on autopsy tissue
9. Terminal coma affects muscarinic receptor mRNA detection in post mortem human temporal cortex
10. Regional and neuronal reductions of polyadenylated messenger RNA in Alzheimer's disease
11. The effects of Alzheimer's disease, other dementias, and premortem course upon amyloid β precursor protein messenger RNAs in frontal cortex
12. Extensive postmortem stability of RNA from rat and human brain
13. Tissue pH as an indicator of mRNA preservation in human post-mortem brain
14. 3H]glutamate binding in the human brain
15. Biological stability of mRNA isolated from human postmortem brain collections
16. Heat-shock 70 messenger RNA levels in human brain: correlation with agonal fever
17. Selective postmortem degradation of inducible heat shock protein 70 (hsp70) mRNAs in rat brain
18. Characterization of messenger RNA extracted from the brains of schizophrenic, depressed and control subjects
19. Human brain neurochemistry: some postmortem problems
20. Brain banking and the human hypothalamus: factors to match for, pitfalls and potentials
21. On the stability of messenger RNA and ribosomal RNA in the brains of control human subjects and patients with Alzheimer's disease
22. Postmortem changes in the levels and localization of microtubule-associated proteins (tau, MAP2 and MAP1B) in the rat and human hippocampus