-
1
-
-
0024289037
-
Laser desorption ionization of proteins with molecular masses exceeding 10,000 Daltons
-
(1988)
Anal Chem
, vol.60
, pp. 2299-2301
-
-
Karas1
Hillenkamp2
-
5
-
-
43949148860
-
Sequencing of peptides in a time-of-flight mass spectrometer: evaluation of postsource decay following matrix-assisted laser desorption ionization (MALDI)
-
Fragmentation caused by excess internal energy can be measured reliably using a reflectron TOF instrument. The fragment ions yield sequence data at subpicomole levels. of outstanding interest
-
(1994)
Int J Mass Spectrom Ion Processes
, vol.131
, pp. 355-385
-
-
Kaufmann1
Kirsch2
Spengler3
-
14
-
-
0028898603
-
Characterization of oligonucleotides and nucleic acids by mass spectrometry
-
An excellent, up-to-date review of mass analysis of nucleic acids, DNA and RNA. of outstanding interest
-
(1995)
Curr Opin Biotechnol
, vol.6
, pp. 96-102
-
-
Limbach1
Crain2
McCloskey3
-
15
-
-
0028727056
-
Matrix-assisted laser desorption/ionization mass spectrometry of oligosaccharides
-
(1994)
Am Laboratory
, vol.12
, pp. 22-28
-
-
Harvey1
-
16
-
-
0028166557
-
Characterization and direct glycoform profiling of a hybrid plasminogen activator by matrix-assisted laser desorption and electrospray mass spectrometry: correlation with high-performance liquid chromatographic and nuclear magnetic resonance analyses of the released glycans
-
(1994)
Biol Mass Spectrom
, vol.23
, pp. 330-338
-
-
Mueller1
Domon2
Karas3
Oostrum4
Richter5
-
17
-
-
0028484542
-
Mass determination of human immunoglobulin IgM using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
-
Protein complexes (982 kDa) of extremely high mass are measured. The mass calibration is corrected for initial velocity of the molecules gained from expansion of the matrix plume during desorption. of special interest
-
(1994)
Rapid Commun Mass Spectrom
, vol.8
, pp. 627-631
-
-
Nelson1
Dogruel2
Williams3
-
21
-
-
84990629710
-
New desorption strategies for the mass spectrometric analysis of macromolecules
-
High sensitivity is achieved by covalent attachment of the matrix to the target. (This work has been difficult to reproduce in other laboratories.) Sensitivity is also enhanced by antibody capture of the analyte molecule. of special interest
-
(1993)
Rapid Communications in Mass Spectrometry
, vol.7
, pp. 576-580
-
-
Hutchens1
Yip2
-
22
-
-
0028167062
-
Laser desorption time-of-flight mass spectrometric analysis of transferrin precipitated with antiserum: a unique simple method to identify molecular weight variants
-
An immunoprecipitated protein complex is measured directly without the need for further sample manipulation. The antibody provides significant, and highly selective, concentration of the analyte. of outstanding interest
-
(1994)
Biol Mass Spectrom
, vol.23
, pp. 230-233
-
-
Nakanishi1
Okamoto2
Tanaka3
Shimizu4
-
23
-
-
0029293298
-
Mass spectrometric immunoassay
-
A mass spectrometer is the read-out device for this immunoassay. The antigen mass provides additional selectivity, and the assay is faster than conventional ELISA and RIA methods, but with equivalent sensitivity. of outstanding interest
-
(1995)
Anal Chem
, vol.67
, pp. 1153-1158
-
-
Nelson1
Krone2
Bieber3
Williams4
-
24
-
-
0028004403
-
Epitope mapping of the gastrin-releasing peptide/anti-bombesin monoclonal antibody complex by proteolysis followed by matrix-assisted laser desorption ionization mass spectrometry
-
The antibody binding site provides protection of the antigenic epitope from proteolytic digestion. Subsequent mass analysis reveals the bound portions of the antigen. of special interest
-
(1994)
Protein Sci
, vol.3
, pp. 1485-1492
-
-
Papac1
Hoyes2
Tomer3
-
26
-
-
0028541590
-
Protein epitope mapping by mass spectrometry
-
Epitope regions are isolated by immunoprecipitation of antigen proteolytic fragments and are subsequently identified by MALDI-MS. of special interest
-
(1994)
Anal Chem
, vol.66
, pp. 3723-3726
-
-
Zhao1
Chait2
-
27
-
-
0028200607
-
Polyvinylidene difluoride (PVDF): an interface for gel electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry
-
(1994)
Biol Soc Trans
, vol.22
, pp. 547-551
-
-
Vestling1
Fenselau2
-
28
-
-
0001123014
-
Poly(vinylidene difluoride) membranes as the interface between laser desorption mass spectrometry, gel electrophoresis, and in situ proteolysis
-
Masses of small proteins may be measured directly from PVDF membranes. The same sample may be proteolytically digested before further analysis. of special interest
-
(1994)
Anal Chem
, vol.66
, pp. 471-477
-
-
Vestling1
Fenselau2
-
29
-
-
12044260066
-
Matrix-assisted laser desorption ionization mass spectrometry of proteins electroblotted after polyacrylamide gel electrophoresis
-
Direct analysis of proteins electroblotted to PVDF is demonstrated. Desorption with infrared radiation and a succinic acid matrix is superior to desorption with uv radiation. of outstanding interest
-
(1994)
Anal Chem
, vol.66
, pp. 464-470
-
-
Strupat1
Karas2
Hillenkamp3
Eckerskorn4
Lottspeich5
-
30
-
-
0027656441
-
Reproducibility and quantitation of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields
-
(1993)
Biol Mass Spectrom
, vol.22
, pp. 544-550
-
-
Preston1
Murray2
Russell3
-
31
-
-
0029046783
-
Peptide mapping by CNBr degradation on a nitrocellulose membrane with analysis by matrix-assisted laser desorption/ionization mass spectrometry
-
(1995)
Anal Chem
, vol.67
, pp. 1705-1710
-
-
Bai1
Qian2
Liu3
Liang4
Lubman5
-
32
-
-
0028774382
-
Matrix-assisted laser desorption/ionization using an active perfluorosulfonated ionomer film substrate
-
(1994)
Anal Chem
, vol.66
, pp. 3423-3430
-
-
Bai1
Liu2
Cain3
Lubman4
-
34
-
-
0029269218
-
Polyethylene membrane as a sample support for direct matrix-assisted laser desorption/ionization mass spectrometric analysis of high mass proteins
-
(1995)
Anal Chem
, vol.67
, pp. 843-848
-
-
Blackledge1
Alexander2
-
35
-
-
0027283520
-
Tryptic mapping of recombinant proteins by matrix-assisted laser desorption/ionization mass spectrometry
-
(1993)
Anal Chem
, vol.65
, pp. 1709-1716
-
-
Billeci1
Stults2
-
37
-
-
0028165537
-
Localization of an O—glycosylated site in the recombinant barley α-amylase 1 produced in yeast and correction of the amino acid sequence using matrix-assisted laser desorption/ionization mass spectrometry of peptide mixtures
-
(1994)
Biol Mass Spectrom
, vol.23
, pp. 547-554
-
-
Andersen1
Sogaard2
Svensson3
Roepstorff4
-
38
-
-
0028071733
-
Microbore reversed-phase high-performance liquid chromatographic purification of peptides for combined chemical sequencing-laser-desorption mass spectrometric analysis
-
MALDI-MS is a valuable complement to Edman degradation, for fraction selection, for sequencer programming, and for improved accuracy of the sequence determination. of special interest
-
(1994)
J Chromatogr [A]
, vol.676
, pp. 121-137
-
-
Elicone1
Lui2
Geromanos3
Erdjument-Bromage4
Tempst5
-
42
-
-
0028914723
-
Isoforms of a cuticular protein from larvae of the meal beetle, Tenebrio molitor, studied by mass spectrometry in combination with Edman degradation and two-dimensional polyacrylamide gel electrophoresis
-
(1995)
Protein Sci
, vol.4
, pp. 394-404
-
-
Haebel1
Jensen2
Andersen3
Roepstorff4
-
43
-
-
0028817534
-
Characterization of Posttranslational Modifications of Brain Tubulin by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry: Direct One-Step Analysis of a Limited Subtilisin Digest
-
(1995)
Analytical Biochemistry
, vol.224
, pp. 532-537
-
-
Rüdiger1
Rüdiger2
Weber3
Schomburg4
-
44
-
-
0028921824
-
Simplified high-sensitivity sequencing of a major histocompatibility complex class I-associated immunoreactive peptide using matrix-assisted laser desorption/ionization mass spectrometry
-
The use of carboxypeptidase with mass analysis of the partially processed substrate yields peptide sequence at subpicomolar levels. of special interest
-
(1995)
Anal Biochem
, vol.226
, pp. 15-25
-
-
Woods1
Huang2
Cotter3
Pasternack4
Pardoll5
Jaffee6
-
45
-
-
0028330012
-
Matrix-assisted laser desorption mass spectrometric peptide mapping of proteins separated by two-dimensional gel electrophoresis: determination of phosphorylation in synapsin I
-
Proteins electroblotted to cationic membranes from 2D gels are cleaved chemically or enzymatically in situ. Mass measurement permits identification of the phosphorylation sites. of special interest
-
(1994)
Protein Sci
, vol.3
, pp. 677-686
-
-
Zhang1
Czernik2
Yungwirth3
Aebersold4
Chait5
-
51
-
-
0028429419
-
Protein identification by peptide mass fingerprinting
-
(1994)
Peptide Res
, vol.7
, pp. 115-124
-
-
Cottrell1
-
52
-
-
0028142375
-
From Electrophoretically Separated Protein to Identification: Strategies for Sequence and Mass Analysis
-
(1994)
Analytical Biochemistry
, vol.221
, pp. 1-15
-
-
Patterson1
-
53
-
-
0002948611
-
Sequence database searching by mass spectrometric data
-
R Kellner, F Lottspeich, HE Meyer, VCH Publishers Inc, Weinheim, An excellent review of peptide mass database search strategies. Key points for proper interpretation of the data are provided. of special interest
-
(1994)
Microcharacterization of Proteins
, pp. 223-245
-
-
Mann1
-
57
-
-
0028575316
-
Error-tolerant identification of peptides in sequence databases by peptide sequence tags
-
The partial peptide sequence derived from fragment ions in the PSD spectrum, together with the mass differences from that sequence to each end of the peptide, yields improved selectivity and error tolerance in mass-based database searching. of outstanding interest
-
(1994)
Anal Chem
, vol.66
, pp. 4390-4399
-
-
Mann1
Wilm2
-
58
-
-
0028240519
-
Identification of transformation sensitive proteins recorded in human two-dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing
-
Proteins from 2-D gels that show transformation-related expression differences are identified by mass-based database searching, either alone or using additional sequence data. of outstanding interest
-
(1994)
Electrophoresis
, vol.15
, pp. 406-416
-
-
Rasmussen1
Mortz2
Mann3
Roepstorff4
Celis5
-
60
-
-
0029022969
-
Matrix-assisted laser-desorption/ionization mass spectrometric approaches for the identification of gel-separated proteins in the 5–50 pmol range
-
(1995)
Electrophoresis
, vol.16
, pp. 1104-1114
-
-
Patterson1
-
61
-
-
0029053727
-
Rapid mass spectrometric peptide sequencing and mass matching for characterization of human melanoma proteins isolated by two-dimensional PAGE
-
A series of proteins that are differentially expressed in melanoma cells are identified by peptide mass database searching or sequencing by tandem mass spectrometry. of outstanding interest
-
(1995)
Proc Natl Acad Sci USA
, vol.92
, pp. 5072-5076
-
-
Clauser1
Hall2
Smith3
Webb4
Andrews5
Tran6
Epstein7
Burlingame8
-
62
-
-
0028960898
-
Identification of myocardial proteins from two-dimensional gels by peptide mass fingerprinting
-
Proteins from human myocardium, separated by 2D gels, are identified by peptide mass database searching. The protein identities form part of a 2D gel database. of outstanding interest
-
(1995)
Electrophoresis
, vol.16
, pp. 308-316
-
-
Sutton1
Pemberton2
Cottrell3
Corbett4
Wheeler5
Dunn6
-
63
-
-
0028170485
-
Peptide sequence information derived by partial acid hydrolysis and matrix-assisted laser desorption/ionization mass spectrometry
-
(1994)
Biol Mass Spectrom
, vol.23
, pp. 734-740
-
-
Vorm1
Roepstorff2
-
64
-
-
0027686255
-
Protein ladder sequencing
-
A series of peptides derived from Edman degradation with partial blocking at each step provide a complete series of ions from which the protein's sequence can be determined. of outstanding interest
-
(1993)
Science
, vol.262
, pp. 89-92
-
-
Chait1
Wang2
Beavis3
Kent4
-
65
-
-
0028501217
-
Peptide ladder sequencing by mass spectrometry using a novel, volatile degradation reagent
-
A volatile reagent for Edman degradation permits high-sensitivity ladder sequencing. Separately, volatile reagents for formation of fixed-charge derivatives yield signal enhancement. of outstanding interest
-
(1994)
Rapid Commun Mass Spectrom
, vol.8
, pp. 737-742
-
-
Bartlet-Jones1
Jeffery2
Hansen3
Pappin4
-
69
-
-
0009757610
-
Protein phosphorylation: sequence-specific identification of in vivo phosphorylation sites by MALDI-TOF mass spectrometry
-
RH Angeletti, Academic Press, Inc, Bronx
-
(1993)
Techniques in Protein Chemistry IV
, pp. 201-210
-
-
Yip1
Hutchens2
-
70
-
-
0028362020
-
An approach to locate phosphorylation sites in a phosphoprotein: mass mapping by combining specific enzymatic degradation with matrix-assisted laser desorption/ionization mass spectrometry
-
Examination of proteolytic peptide masses before and after phosphatase treatment reveals phosphorylated pept de(s) as those that display mass difference of 80 Da. of special interest
-
(1994)
Anal Biochem
, vol.219
, pp. 9-20
-
-
Liao1
Leykam2
Andrews3
Gage4
Allison5
-
71
-
-
0028166698
-
Monitoring protein kinase and phosphatase reactions with matrix-assisted laser desorption/ionization mass spectrometry and capillary zone electrophoresis: comparison of the detection efficiency of peptide-phosphopeptide mixtures
-
(1994)
Biol Mass Spectrom
, vol.23
, pp. 519-528
-
-
Craig1
Hoeger2
Miller3
Goedken4
Rivier5
Fischer6
-
72
-
-
84982608980
-
Distinction between phosphorylated and sulfated peptides by matrix-assisted laser desorption/ionization reflector mass spectrometry at the subpicomole level
-
JW Crabb, Academic Press, San Diego, Differences in mass between the fragment ions measured using reflector- and linear-TOF instruments can distinguish between phospho- and sulfopeptides, which have nominally identical masses. of outstanding interest
-
(1994)
Techniques in Protein Chemistry V
, pp. 105-113
-
-
Talbo1
Mann2
-
76
-
-
0028544044
-
Prompt Fragmentation of Disulfide-Linked Peptides during Matrix-Assisted Laser Desorption Ionization Mass Spectrometry
-
(1994)
Analytical Chemistry
, vol.66
, pp. 3727-3732
-
-
Patterson1
Katta2
-
77
-
-
0028906033
-
Facile, in situ matrix-assisted laser desorption ionization-mass spectrometry analysis and assignment of disulfide pairings in heteropeptide molecules
-
(1995)
Anal Biochem
, vol.226
, pp. 355-361
-
-
Crimmins1
Saylor2
Rush3
Thoma4
-
78
-
-
0000795726
-
Quantitative assessment of cysteine and cystine in peptides and proteins following organomercurial derivatization and analysis by matrix-assisted laser desorption ionization mass spectrometry
-
(1994)
J Am Soc Mass Spectrom
, vol.5
, pp. 359-366
-
-
Zaluzec1
Gage2
Watson3
-
79
-
-
0027918090
-
Site-specific carbohydrate identification in recombinant proteins using MALDI-TOF MS
-
(1993)
Anal Chem
, vol.65
, pp. 2791-2800
-
-
Huberty1
Vath2
Yu3
Martin4
-
80
-
-
0028206873
-
The oligosaccharides of the Fe(III)-Zn(II) purple acid phosphatase of the red kidney bean
-
Mass analysis of peptides generated by sequential CNBr and trypsin cleavages permits identification of the glycopeptides by their heterogeneous masses. The identities are confirmed by immunoassay. Carbohydrate composition is determined from the mass of the oligosaccharide portion of the molecule whereas sequential exoglycosidase treatments yields the carbohydrate sequence. of special interest
-
(1994)
Eur J Biochem
, vol.220
, pp. 321-330
-
-
Stahl1
Klabunde2
Witzel3
Krebs4
Steup5
Karas6
Hillenkamp7
-
81
-
-
0028349781
-
Site-specific characterization of glycoprotein carbohydrates by exoglycosidase digestion and laser desorption mass spectrometry
-
Mass analysis of the isolated glycopeptides after each step of sequential exoglycosidase digestion yields the sequence and linkages of the carbohydrate portion. of special interest
-
(1994)
Anal Biochem
, vol.218
, pp. 34-46
-
-
Sutton1
O'Neill2
Cottrell3
-
82
-
-
0028028837
-
A method to increase contaminant tolerance in protein matrix-assisted laser desorption/ionization by the fabrication of thin protein-doped polycrystalline films
-
Drying of the sample on to a thin film of matrix permits washing of the analyte to yield greater tolerance to salts and other buffer components. of special interest
-
(1994)
Rapid Communications in Mass Spectrometry
, vol.8
, pp. 199-204
-
-
Xiang1
Beavis2
-
83
-
-
0000094029
-
Improved resolution and very high sensitivity in MALDI TOF of matrix surfaces made by fast evaporation
-
A thin layer of matrix brings subfemtomolar detection limits, enhanced resolution, and, upon washing, a greater tolerance for contaminating buffer components. of outstanding interest
-
(1994)
Anal Chem
, vol.66
, pp. 3281-3287
-
-
Vorm1
Roepstorff2
Mann3
-
84
-
-
0001305955
-
Improved mass accuracy in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides
-
Fast-evaporation matrix preparation produces a very uniform surface which yields more uniform desorption/ionization, resulting in greater mass accuracy for external calibration. The use of matrix ions for internal mass calibration leads to further improvement in mass accuracy. of outstanding interest
-
(1994)
J Am Soc Mass Spectrom
, vol.5
, pp. 955-958
-
-
Vorm1
Mann2
-
87
-
-
84993884569
-
Enhanced detection of peptides in matrix-assisted laser desorption/ionization mass spectrometry through the use of charge-localized derivatives
-
(1995)
Journal of Mass Spectrometry
, vol.30
, pp. 511-512
-
-
Liao1
Allison2
-
88
-
-
0028346026
-
Mass spectrometric molecular-weight determination of highly acidic compounds of biological significance via their complexes with basic polypeptides
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 4333-4337
-
-
Juhasz1
Biemann2
-
93
-
-
0028335238
-
Quantitative determination of proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
-
The proper use of internal standards permits quantification of proteins at very low concentrations. of special interest
-
(1994)
Anal Chem
, vol.66
, pp. 1408-1415
-
-
Nelson1
McLean2
Hutchens3
-
98
-
-
0029331042
-
Mass resolution improvement by incorporation of pulsed ion extraction in a matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometer
-
Delayed extraction of ions following the laser pulse provides improved mass resolution with excellent sensitivity. of outstanding interest
-
(1995)
Anal Chem
, vol.67
, pp. 1998-2003
-
-
Brown1
Lennon2
-
99
-
-
84990710971
-
Improving the resolution of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry by exploiting the correlation between ion position and velocity
-
A pulsed-ion draw-out field (delayed extraction) following the laser pulse provides significant enhancement in resolution. of outstanding interest
-
(1994)
Rapid Communications in Mass Spectrometry
, vol.8
, pp. 865-868
-
-
Colby1
King2
Reilly3
-
103
-
-
0029644557
-
Sequence-specific fragmentation generated by matrix-assisted laser desorption/ionization in a quadrupole ion trap/reflectron time-of-flight device
-
The long ion storage time made possible by ion traps permit a greater degree of fragmentation than does conventional low-energy collision-induced dissociation. of special interest
-
(1995)
Anal Chem
, vol.67
, pp. 1400-1408
-
-
Lee1
Lubman2
-
106
-
-
84910900588
-
-
Doroshenko VM, Cotter RJ: A new method of trapping ions produced by matrix-assisted laser desorption ionization in a quadrupole ion trap. 1993, 7:822–827.
-
-
-
-
109
-
-
0028363368
-
High resolution laser desorption mass spectrometry of peptides and proteins
-
Ultra-high resolution (m/Δm〉1,000,000) which allows separation of isotope peaks and high mass accuracy can be achieved using MALDI-FTMS. of special interest
-
(1994)
Proc Natl Acad Sci USA
, vol.91
, pp. 4801-4805
-
-
McIver1
Li2
Hunter3
-
113
-
-
0029643888
-
High-mass analysis using quadrupolar excitation/ion cooling in a Fourier transform mass spectrometer
-
(1995)
Anal Chem
, vol.67
, pp. 379-384
-
-
Pastor1
Castoro2
Wilkins3
-
115
-
-
0028473066
-
High-accuracy molecular mass determination for peptides and proteins by Fourier transform mass spectrometry
-
(1994)
Anal Chem
, vol.66
, pp. 2077-2083
-
-
Li1
McIver2
Hunter3
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