Mechanism of action of hexarelin and GHRP-6: Analysis of the involvement of GHRH and somatostatin in the rat

Neuroendocrinology

Volumn 61, Issue 1, 1995, Pages 44-50

  Conley, Lisa K ^a   Teika, Jo Anne ^a   Deghenghi, Romano ^b   Imbimbo, Bruno P ^c   Giustina, Andrea ^d   Locatelli, Vittorio ^e   Wehrenberg, William B ^a  

^a UNIVERSITY OF WISCONSIN MILWAUKEE   (United States)

^b Ardana   (France)

^c Mediolanum Farmaceutici   (Italy)

^d UNIVERSITY OF BRESCIA   (Italy)

^e UNIVERSITY OF MILAN   (Italy)

Author keywords

GHRP 6; Growth hormone; Growth hormone releasing hormone; Hexarelin; Somatostatin

Indexed keywords

ANTIBODY; GROWTH HORMONE; GROWTH HORMONE RELEASING FACTOR; PEPTIDE; SOMATOSTATIN; SOMATOSTATIN ANTIBODY;

ANIMAL EXPERIMENT; AREA UNDER THE CURVE; ARTICLE; BLOOD SAMPLING; CATHETER; CONTROLLED STUDY; DATA ANALYSIS; GROWTH HORMONE BLOOD LEVEL; GROWTH HORMONE RELEASE; HORMONE ACTION; HYPOPHYSIS; HYPOTHALAMUS; JUGULAR VEIN; MALE; NEUROENDOCRINE SYSTEM; NONHUMAN; PRIORITY JOURNAL; RAT; SOMATOSTATIN RELEASE;

AMINO ACID SEQUENCE; ANIMAL; GROWTH SUBSTANCES; IMMUNIZATION, PASSIVE; MALE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; RATS; RATS, SPRAGUE-DAWLEY; SOMATOSTATIN; SOMATOTROPIN-RELEASING HORMONE;

EID: 0028833447     PISSN: 00283835     EISSN: 14230194     Source Type: Journal    
DOI: 10.1159/000126826     Document Type: Article

References (57)

1. Rivier J. Spiess J, Thorner M. Vale W: Characterization of a growth hormone-releasing factor from a human pancreatic islet tumour. Nature 1982;300:276-278.
2. Guillemin R. Brazeau P. Bohlen P. Esch F, Ling N, Wehrenberg WB: Growth hormone-releasing factor from a human pancreatic tumor that caused acromegaly. Science 1982;218: 585-587.
3. Spiess J. Rivier J, Vale W: Characterization of rat hypothalamic growth hormone-releasing factor. Nature 1983:303:532-535.
4. Brazeau P, Vale W. Riirgus R, Ling N. Butcher M. Rivier J. Guillemin R: Hypothalamic polypeptide that inhibits the secretion of immuno-reactive pituitarv growth hormone. Science 1973;179:77-79.
5. Brazeau P. Ling N. Bohlen P. Esch F, Ying S, Guillemin R: Growth hormone releasing factor, somatocrinin, releases pituitary growth hormone in vitro. Proc Natl Acad Sci USA 1982;79:7909-7913.
6. Vale W, Vaughan J, Yamamoto G, Spiess J. Rivier J: Effects of synthetic human pancreatic (tumor) GH releasing factor and somatostatin, triiodothyronine and dexamethasone on GH secretion in vitro. Endocrinology 1983:112: 1553-1555.
7. Roemer D, Buescher HH. Hill RC, Pless J. Bauer W. Cardinaux F, Closse A, Hauser D, Hugucnin R: A synthetic enkephalin analogue with prolonged parenteral and oral analgesic activity. Nature 1977;268:547-549.
8. Bowers CY, Momany F, Reynolds GA, Chang D. Hong A. Chang K: Structure-activity relationships of a synthetic pcntapeptide that specifically releases growth hormone in vitro. Endocrinology 1980:106:663-667.
9. Momany F, Bowers CY. Reynolds GA, Hong A, Newlander K: Conformational energy studies and in vitro and in vivo activity data on growth hormone-releasing peptides. Endocrinology 1984;114:1531-1536.
10. Bowers CY. Reynolds GA. Chang D. Hong A, Chang K, Momany F: A study on the regulation of growth hormone release from the pituitaries of rats in vitro. Endocrinology 1981; 108:1071-1080.
11. Bowers CY. Momany F. Reynolds GA. Hong A: On the in vitro and in vivo activity of a new synthetic hexapcptide that acts on the pituitary' to specifically release growth hormone. Endocrinology 1984;114:1537-1545.
12. Badger TM, Millard WJ. McCormick GF, Bowers CY, Martin JB: The effects of growth hormone (GH)-releasing peptides on GH secretion in perifused pituitary cells of adult male rats. Endocrinology 1984; 115:1432-1438.
13. Ilson BE. Jorkasky DK.Cumow RT, Stote RM: Effect of a new synthetic hcxapeptide to selectively stimulate growth hormone release in healthy human subjects. J Clin Endocrinol Me-tab 1989;69:212-214.
14. Walker RF, Codd EE, Barone FC. Nelson AH, Goodwin T. Campbell SA: Oral activity of the growth hormone releasing peptide His-D-Trp-Ala-Trp-D-Phe-Lys-NH? in rats, dogs and monkeys. Life Sci 1990:47:29-36.
15. Bowers CY, Reynolds GA, Durham D, Barrera CM, Pezzoli SS, Thomer MO: Growth hormone (GH)-relcasing peptide stimulates GH release in normal men and acts synergistically with GH-releasing hormone. J Clin Endocrinol Metab 1990;70:975-982.
16. Malozowski S. Hao EH, Ren SG. Marin G, Liu L, Southers JL, Merriam GR: Growth hormone (GH) responses to the hexapeptide GH-releasing peptide and GH-releasing hormone (GHRH) in the cvnomolgus macaque: Evidence for non-GHRH-mediated responses. J Clin Endocrinol Metab 1991;73:314-317.
17. Hartman ML. Farello G, Pezzoli SS, Thomer MO: Oral administration of growth hormone (GH)-releasing peptide stimulates GH secretion in normal men. J Clin Endocrinol Metab 1992;74:1378-1384.
18. Conley LK, Giustina A, Imbimbo BP, Stagg LC, Deghcnghi R. Wehrenberg WB: Hexarelin [His-D-2Methyl-Trp-Ala-Trp-D-Phe-Lys-NHi], a new GH-releasing peptide, is biologically active in male and female rats. Endocr J 1994;in press.
19. Clark RG, Carlsson LMS, Trojnar J, Robinson ICAF: The effects of a growth hormone-releas-ing peptide and growth hormone-releasing factor in conscious and anaesthetized rats. J Neuroendocrinology 1989;1:249-255.
20. Bowers CY. Sartor AO, Reynolds GA, Badger TM: On the actions of the growth hormone-releasing hexapeptide, GHRP. Endocrinology 1991;128:2027-2035.
21. Dickson SL, Lcng G, Robinson IC: Systemic administration of growth hormone releasing peptide activates hypothalamic arcuate neurons. Neuroscience 1993:53:303-306.
22. Debell WK, Pezzoli SS, Thomer MO: Growth hormone (GH) secretion during continuous infusion of GH-relcasing peptide: Partial response attenuation. J Clin Endocrinol Metab 1991;72:1312-1316.
23. Sartor O, Bowers CY, Reynolds GA, Momany F: Variables determining the growth hormone response of His-Ö-Trp-Ala-Trp-Ö-Phc-Lys-NH2 in the rat. Endocrinology 1985; 117:1441-1447.
24. Goth Ml, Lyons CE, Canny BJ, Thorner MO: Pituitary adenylate cyclase activating polypeptide. growth hormone (GH)-releasing peptide and GH-releasing hormone stimulate GH release through distinct pituitary receptors. Endocrinology 1992;130:939-944.
25. Oborsawin MC, Shin SH, Arrowsmith J: Surgery depresses pulsatile growth hormone release in rats for up to 2 days. Acta Endocrinol (Copcnh) 1985;110:42-45.
26. Wehrenberg WB. Brazeau P, Luben R, Ling N. Guillemin R: A noninvasive functional lesion of the hypothalamo-pituitary axis for the study of growth hormone-releasing factor. Neurocn-docrinology 1983:36:489-491.
27. Wehrenberg WB. Brazeau P. Ling N. Textor G, Guillemin R: Pituitary growth hormone response in rats during a 24-hour infusion of growth hormone-releasing factor. Endocrinology 1984;114:1613-1616.
28. Benoit R, Bohlen P, Ling N. Brisken A, Esch F, Brazeau P, Ying SY, Guillemin R: Presence of somatostatin-28-(l-12) in hypothalamus and pancreas. Proc Natl Acad Sci 1982:79:917-921.
29. Wehrenberg WB: The role of growth hormone-releasing factor and somatostatin on somatic growth in rats. Endocrinology 1986:118:489-494.
30. Rorstad OP, Epelbaum J. Brazeau PM, Martin JB: Chromatographic and biological properties of immunoreactivc somatostatin in hypothalamic and extrahypothalamic brain regions of the rat. Endocrinology 1979;105:1083-1092.
31. Wehrenberg WB, Voitz DM, Celia SG. Muller EE, Gaillard RC: Long-term failure of compensatory growth in rats following acute neonatal passive immunization against growth hor-monc-relcasing hormone. Neuroendocrinologv 1992, 56:509-515.
32. Wehrenberg WB. Brazcau P, Lubcn R, Bohlcn P. Guillemin R: Inhibition of the pulsatile secretion of growth hormone by monoclonal antibodies to the hypothalamic growth hormone releasing factor (GRF). Endocrinology 1982;111:2147-2148.
33. Wehrenberg WB. Ling N. Bohlen P. Esch F. Brazcau P, Guillemin R: Physiological roles of somatocrinin and somatostatin in the regulation of growth hormone secretion. Biochem Biophys Res Commun 1982; 109:562-567.
34. Plotsky PM. Vale W: Patterns of growth hormone-releasing factor and somatostatin secretion into the hypophysial-portal circulation of the rat. Science 1985;230:461-463.
35. Terry LC, Willoughby JO, Brazcau P, Martin JB, Patel Y: Antiserum to somatostatin prevents stress-induced inhibition of growth hormone secretion in the rat. Science 1976:192: 565-566.
36. Demarest KT, Moore KE, Ricgle GD: Restraint stress decreases the neurosecretory activity of tuberoinfundibular dopaminergic neurons in young but not in aged female rats. Neuroendocrinologv 1987;45:333-337.
37. Sartor O. Bowers CY, Chang D: Parallel studies of His-DTrp-Ala-Trp-DPhe-Lys-NH2 and human pancreatic growth hormone-releasing fac-tor-44-NHi in rat primary pituitary cell monolayer culture. Endocrinology 1985; 116:952-957.
38. Cheng K, Chan WWS, Barreto A, Convey EM, Smith RG: The synergistic effects of His-D-Trp-Ala-Trp-D-Phe-Lys-NHî on growth hormone (GH)-releasing factor-stimulated GH release and intracellular adenosine 3', 5'-mono-phosphate accumulation in rat primary pituitary cell culture. Endocrinology 1989; 124: 2791-2798.
39. Blake AD. Smith RG: Dcsensitization studies using perifused rat pituitary cells show that growth hormone-releasing hormone and His-D-Trp-Ala-Trp-ü-Phe-Lys-N H2 stimulate growth hormone release through distinct receptor sites. J Endocrinol 1991; 129:11-19.
40. Codd EE, Shu AYL. Walker RF: Binding of a growth hormone releasing hexapeptide to specific hypothalamic and pituitary binding sites. Neuropharmacology 1989:28:1139-1144.
41. Locatelli V, Torselío A. Grille R. Ghigo MC, Celia SG, Deghenghi R, Wehrenberg WB, Muller EE: GHRP-6 stimulates GH secretion and synthesis independently from endogenous GHRH and SRIF in the infant rat. J Endocrinol Invest 1993;16(suppl): 107.
42. Chihara K, Minamitani N, Kaji H, Kodama H. Kita T. Fujita T: Noradrenergic modulation of human pancreatic growth hormone-releasing factor (hpGHRFl-44)-induced growth hormone release in conscious male rabbits: Involvement of endogenous somatostatin. Endocrinology 1984; 114:1402-1406.
43. Schalch DS, Reichlin S: Plasma growth hormone concentration in the rat determined by radioimmunoassay: Influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants. Endocrinology 1966;79:275-280.
44. Arimura A, Smith WD. Schally AV: Blockade of the stress-induced decrease in blood GH by anti-somatostatin serum in rats. Endocrinology 1976;98:540-543.
45. Fukata J, Diamond DJ. Martin JB: Effects of rat growth hormone (rGH)-releasing factor and somatostatin on the release and synthesis of rGH in dispersed pituitary cells. Endocrinology 1985;117:457-467.
46. Clark RG, Carlsson LMS, Robinson ICAF: Growth hormone (GH) secretion in the conscious rat: Negative feedback of GH on its own release. J Endocrinol 1988; 119:201-209.
47. Wehrenberg WB. Bloch B, Ling N: Pituitary secretion of growth hormone in response to opioid peptides and opiates is mediated through growth hormone-releasing factor. Neu-roendocrinology 1985;41:13-16.
48. Mounier F, Bluet-Pajot MT. Durand D, Schaub C: Effects of acute stress on growth hormone release induced by morphine and cloni-dinc in the rat. Neuroendocrinol Lett 1983:5: 221-226.
49. Ndon JA, Giustina A. Wehrenberg WB: Hypothalamic regulation of impaired growth hormone secretion in diabetic rats. 1. Studies in streplozotocin-induced diabetic rats. Neuroendocrinologv 1992:55:500-505.
50. Ndor, JA, Giustina A, Wehrenberg WB: Hypothalamic regulation of impaired growth hormone secretion in diabetic rats. 2. Studies in spontaneously diabetic BB Worcester rats. Neuroendocrinology 1992;55:506-511.
51. Patel YC, Wheatley T, Zingg HH: Increased blood somatostatin concentration in streptozo-tocin diabetic rats. Life Sci 1980:27:1563-1570.
52. Patel YC, Wheatley T, Malaisse-Lagae F, Orci L: Elevated portal and peripheral blood concentration of immunoreactive somatostatin in spontaneously diabetic (BBL) Wistar rats. Suppression with insulin. Diabetes 1980;29:757-761.
53. Giustina A, Bussi AR, Conti C, Doga M, Legati F, Macca C, Zuccato F. Wehrenberg WB: Comparative effect of galanin and pyridostigmine on the growth hormone response to growth hor-mone-releasing hormone in normal aged subjects. Horm Res 1992;37:165-170.
54. Sonntag WE, Gough MA: Growth hormone releasing hormone induced release of growth hormone in aging male rats: Dependence on pharmacological manipulation and endogenous somatostatin release. Neuroendocrinolo-gy 1988;47:482-488.
55. Sonntag WE, Steger RW, Forman LJ, Meites J: Decreased pulsatile release of growth hormone in old male rats. Endocrinology 1980; 107: 1875-1879.
56. Giustina A, Wehrenberg WB: The role of glucocorticoids in the regulation of growth hormone secretion: Mechanisms and clinical significance. Trends Endocrinol Metab 1992;3:306-311.
57. Giustina A, Licini M, Girelli A, Bussi AR, Wehrenberg WB: Effect of galanin and arginine, alone or in combination, on growth hormone secretion in adult patients treated with glucocorticoids. Ncuroendocrinology 1993;57: 843-848.