Reliability of differential PCR for the detection of EGFR and MDM2 gene amplification in DNA extracted from FFPE glioma tissue

Journal of Neuropathology and Experimental Neurology

Volumn 54, Issue 1, 1995, Pages 57-64

  Hunter, Stephen B ^a   Abbott, Karen ^a   Varma, Vijay A ^a   Olson, Jeffrey J ^a   Barnett, David W ^a   James, C David ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Amplification; Archival; Differential PCR; Oncogene; Slot blot

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR;

ARTICLE; DNA DETERMINATION; DOT HYBRIDIZATION; GENE AMPLIFICATION; GENE EXPRESSION; GLIOMA; HUMAN; HUMAN CELL; HUMAN TISSUE; ONCOGENE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ADULT; AGED; BASE SEQUENCE; DNA, NEOPLASM; EVALUATION STUDIES; FEMALE; FIXATIVES; FORMALDEHYDE; GENE AMPLIFICATION; GLIOMA; HUMAN; MALE; MIDDLE AGE; MOLECULAR PROBES; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; PROTO-ONCOGENE PROTEINS; RECEPTOR, EPIDERMAL GROWTH FACTOR; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0028832159     PISSN: 00223069     EISSN: 15546578     Source Type: Journal    
DOI: 10.1097/00005072-199501000-00007     Document Type: Article

References (29)

1. Look AT, Hayes FA, Shuster JJ, et al. Clinical relevance of tumor cell ploidy and N-myc gene amplification in childhood neuroblastoma: A Pediatric Oncology Group study. J Clin Oncol 1991;9: 581-91
2. Slamon DJ, Clark GM, Wong SG, Levin WJ, Ullrich A, McGuire WL. Human breast cancer: Correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science 1987;235: 177-82
3. Wong AJ, Bigner SH, Bigner DD, Kinzler KW, Hamilton SR, Vo-gelstein B. Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification. Proc Natl Acad Sci USA 1987;84:6899-903
4. Strommer K, Hamou MF, Diggelmann H, de Tribolet N. Cellular and tumoural heterogeneity of EGFR gene amplification in human malignant gliomas. Acta Neurochir 1990;107:82-7
5. Ekstrand AJ, James CD, Cavenee WK, Seliger B, Pettersson RF, Collins VP. Genes for epidermal growth factor receptor, transforming growth factor-alpha, and epidermal growth factor and their expression in human gliomas in vivo. Cancer Res 1991;51:2164-72
6. Chaffanet M, Chauvin C, Laine M, et al. EGF receptor amplification and expression in human brain tumours. Eur J Cancer 1992;28: 11-17
7. Torp SH, Helseth E, Dalen A, Unsgaard G. Relationships between Ki-67 labelling index, amplification of the epidermal growth factor receptor gene, and prognosis in human glioblastomas. Acta Neurochir 1992;117:182-6
8. Schlegel J, Merdes A, Stumm G, et al. Amplification of the epidermal-growth-factor-receptor gene correlates with different growth behaviour in human glioblastoma. Int J Cancer 1994;56:72-7
9. Reifenberger G, Liu L, Ichimura K, Schmidt EE, Collins VP. Amplification and overexpression of the MDM2 gene in a subset of human malignant gliomas without p53 mutations. Cancer Res 1993;53:2736-9
10. Momand J, Zambetti GP, Olson DC, George D, Levine AJ. The MDM2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation. Cell 1992;69:1237-45
11. Oliner JD, Pietenpol JA, Thiagalingam S, Gyuris J, Kinzler KW, Vogelstein B. MDM2 conceals the activation domain of p53. Nature 1993;362:857-60
12. Bigner SH, Burger PC, Wong AJ, et al. Gene amplification in malignant human gliomas: Clinical and histopathological aspects. J Neuropathol Exp Neurol 1988;47:191-205
13. Hurtt MR, Moossy J, Donovan-Peluso M, Locker J. Amplification of epidermal growth factor receptor gene in gliomas: Histopathol-ogy and prognosis. J Neuropathol Exp Neurol 1992;51:84-90
14. von Deimling A, von Ammon K, Schoenfeld D, Wiestler OD, Sei-zinger BR, Louis DN. Subsets of glioblastoma multiforme defined by molecular genetic analysis. Brain Pathol 1993;3:19-26
15. Neubauer A, Neubauer B, He M, et al. Analysis of gene amplification in archival tissue by differential polymerase chain reaction. Oncogene 1992;7:1019-25
16. Hruza C, Dobianer K, Beck A, et al. HER-2 and INT-2 amplification estimated by quantitative PCR in paraffin-embedded ovarian cancer tissue samples. Eur J Cancer 1993;29A: 1593-7
17. Kleihues P, Burger PC, Scheithauer B. The new WHO classification of brain tumours. Brain Pathol 1993;3:255-68
18. Ullrich A, Coussens L, Hayflick JS, et al. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 1984;309: 418-25
19. Oliner JD, Kinzler KW, Meltzer PS, Georger DL, Vogelstein B. Amplification of a gene encoding p53-associated protein in human sarcomas. Nature 1992;358:80-3
20. Grandy DK, Marchionni MA, Makam H, et al. Cloning of the cDNA and gene for a human D, dopamine receptor. Proc Natl Acad Sci USA 1989;86:9762-6
21. Lawn RM, Efstratiadis A, O'Connell C, Maniatis T. The nucleotide sequence of the human beta-globin gene. Cell 1980;21:647-51
22. Riordan JR, Rommens JM, Kerem B, et al. Identification of the cystic fibrosis gene: Cloning and characterization of complementary DNA. Science 1989;245:1066-73
23. James CD, Carlbom E, Dumanski J, et al. Clonal genomic alterations in glioma malignancy stages. Cancer Res 1988;48:5546-51
24. Feinberg AP, Vogelstein B. A technique for radiolabeling DNA re- striction endonucleasc fragments to high specific activity. Anal Bio-chem 1983;132:6-13
25. Xu YH, Ishii S, Clark AJ, et al. Human epidermal growth factor receptor cDNA is homologous to a variety of RNAs overproduced in A431 carcinoma cells. Nature 1984;309:806-10
26. Filmus J, Pollak MN, Cailleau R, Buick RN. MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF. Biochem Biophys Res Commun 1985;128:898-905
27. He J, Reifenberger G, Lu L, Collins VP, James CD. Analysis of glioma cell lines for amplification and overexpression of MDM2. Genes Chromosom Cancer 1994; 11:91-6
28. Ekstrand AJ, Longo N, Hamid ML, Olson JJ, Collins VP, James CD. Functional characterization of an EGF receptor with a truncated extracellular domain expressed in glioblastomas with EGFR gene amplification. Oncogene 1994;9:2313-20
29. Gilbert J, Norris MD, Haber M, Kavallaris M, Marshall GM, Stewart BW. Determination of N-myc gene amplification in neuroblastoma by differential polymerase chain reaction. Mol Cell Probes 1993;7:227-34